I'm interested in your antibody CD31/PECAM1 ref: NB100-2284. I'd like to stain human endothelial cells injected in mouse muscle. Do you know if your antibody cross-reacts with mouse? Is this antibody working on Immunohistochemistry frozen sections?

NB100-2284 reacts with human and mouse in WB, immunocytochemistry and IHC on both frozen and paraffin-embedded tissues. Novus also offers sample sizes of several products, which may be identified by a SS at the end of the catalog number (ie. NB100-105SS is the sample size of NB100-105). We also offer Super Novus packs to many common antigens. These kits enable customers to test several different antibodies to the target at a discounted rate.

I'm interested in your antibody CD31/PECAM1 ref: NB100-2284. I'd like to stain human endothelial cells injected in mouse muscle. Do you know if your antibody cross-reacts with mouse? Is this antibody working on Immunohistochemistry frozen sections?

As is listed on our datasheet, NB100-2284 reacts with human and mouse in WB, immunocytochemistry and IHC on both frozen and paraffin-embedded tissues. Novus also offers sample sizes of several products, which may be identified by a "SS" at the end of the catalog number (ie. NB100-105SS is the sample size of NB100-105). We also offer Super Novus packs to many common antigens. These kits enable customers to test several different antibodies to the target at a discounted rate.

I am looking for references concerning the use of NBP2-15797, NBP1-47913 and NB100-2284 in IHC on PFA- fixed section.

Among your mentioned catalog numbers (NBP2-15797, NBP1-47913 and NB100-2284), we have published references for NB100-2284 only. Hylander et al. 2013 (Journal of Translational Medicine 2013, 11:110) have cited the IHC-P use of NB100-2284 and this paper has also explained a nice detailed IHC-P protocol that you may follow if you want to. NBP1-71663 is our bestselling catalog number among all CD31/PECAM1 antibodies and it has been cited in two publications, both explaining its use in IHC-P assays (Pratheeshkumar et al. 2012 PLoS One. 2012;7(10):e47516; and Pratheeshkumar et al. 2012 PLoS One. 2012;7(12):e52279)

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CD31/PECAM-1 Antibody

Name: CD31/PECAM-1 Antibody
Brand: Novus Biologicals
SKU: NB100-2284
Rating: 4.7 (6 reviews)

(133 Publications)

(6 Reviews)

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FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit CD31/PECAM-1 Antibody (NB100-2284) used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG-Hilyte PlusTM 555

Staining of CD31/PECAM-1 in Rat Liver sample. IHC-P image submitted by a verified customer review.

Human lung adenocarcinoma using. Antibody at 1:100 with citrate epitope retrieval at pH 6.0.

Analysis in rat muscle blood vessels using. IHC-P image submitted by a verifed customer review.

Matrix-HA supplemented with strontium was implanted subcutaneously in mice, where immunochemistry analysis and histology occurred of the newly formed tissues. (C) Within each matrix: Matrix-HA, Matrix-8Sr-HA and ...read more

Imaging of HUVEC monolayer on glass substrate. Fixation with 4% PFA. Blocking with Goat Serum at 1:100. Incubation 2 hours at room temperature. ICC/IF image submitted by a verified customer review.

Analysis of canine kidney tissue using CD31/PECAM-1 antibody. Antibody was used at a 1:50 concentration in Casein in PBS and left at 4C overnight on paraffin embedded canine bladder tissue. HIER was performed in ...read more

Analysis in pig SC cells using CD31/PECAM-1 Antibody (red). Blue color showing nucleus labeling. ICC/IF image submitted by a verified customer review.

Immunohistochemistry evaluation of CD31/PECAM-1 expression. Representative images of CD31/PECAM-1 staining of vessels in the three groups of implanted matrices (scale bar = 100 um). Image collected and cropped by CiteAb ...read more

CD31 staining on E11.5 mouse Pharyngeal Mesoderm. Fixed with 4% PFA overnight. Blocked with 1% BSA. Primary antibody at 1:100. Secondary antibody at 1:1000, conjugated to Alexa Fluor 488. IHC-Fr image submitted by a ...read more

Immunohistochemistry evaluation of CD31 expression. (a) Representative images of CD31 staining of vessels in the three groups of implanted matrices (scale bar = 100 µm). (b) Quantification of vessel density ...read more

Immunohistochemistry: Rabbit Polyclonal CD31/PECAM-1 Antibody [NB100-2284] - Staining of CD31 in human lung tissue. Nuclei in blue and CD31 (vessel) in green. Mouse anti-CD31 (1:100, overnight incubation at +4°C), ...read more

Product Details

Summary

Reactivity	Hu, Mu, Rt, Po, CaSpecies Glossary
Applications	WB, ICC/IF, IHC
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	0.1 mg/ml

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CD31/PECAM-1 Antibody Summary

Immunogen	The immunogen recognized by this CD31/PECAM-1 Antibody maps to a region between residue 700 and the C-terminus (residue 738) of human CD31 using the numbering given in entry NP_000433.2 (Gene ID 5175).
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	PECAM1
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Immunocytochemistry/ Immunofluorescence 1:50 - 1:500
Immunohistochemistry 1:100 - 1:500
Immunohistochemistry-Frozen 1:10 - 1:500
Immunohistochemistry-Paraffin 1:100 - 1:500
Western Blot 1:100 - 1:2000

Application Notes

For IHC-P: Epitope exposure is recommended, with citrate buffer will enhance staining. In some cases, the antibody may be diluted further than indicated. IHC-Fr, WB reactivity reported in scientific literature (PMID:23317813). ICC/IF reactivity reported in scientific literature (PMID: 27328066).

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 6 Reviews rated 4.7

using
NB100-2284 in the following applications:

Immunohistochemistry-Paraffin
Immunohistochemistry-Frozen
Immunocytochemistry

Publications

Read Publications using
NB100-2284 in the following applications:

FLOW
1 publication
ICC/IF
25 publications
IF
1 publication
IF/IHC
23 publications
IHC
8 publications
IHC-Fr
8 publications
IHC-P
22 publications
WB
10 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23317813). Rat reactivity reported in scientific literature (PMID: 29960821). Porcine reactivity reported from a verified customer review. Canine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage	Store at 4C. Do not freeze.
Buffer	TBS, 0.1% BSA
Preservative	0.09% Sodium Azide
Concentration	0.1 mg/ml
Purity	Immunogen affinity purified

Alternate Names for CD31/PECAM-1 Antibody

adhesion molecule
CD31 antigen
CD31
CD31/EndoCAM
EndoCAM
FLJ34100
FLJ58394
GPIIA'
PECA1
PECAM1
PECAM-1
PECAM-1, CD31/EndoCAM
platelet endothelial cell adhesion molecule
platelet endothelial cell adhesion molecule-1
platelet/endothelial cell adhesion molecule

Background

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (NB100-2284)(133)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Mammal, Xenopus.

We have publications tested in 8 applications: FLOW, ICC/IF, IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 133. Show All 133 Publications.

Publications using NB100-2284	Applications	Species
Nadia Houerbi, JangKeun Kim, Eliah G. Overbey, Richa Batra, Annalise Schweickart, Laura Patras, Serena Lucotti, Krista A. Ryon, Deena Najjar, Cem Meydan, Namita Damle, Christopher Chin, S. Anand Narayanan, Joseph W. Guarnieri, Gabrielle Widjaja, Afshin Beheshti, Gabriel Tobias, Fanny Vatter, Jeremy Wain Hirschberg, Ashley Kleinman, Evan E. Afshin, Matthew MacKay, Qiuying Chen, Dawson Miller, Aaron S. Gajadhar, Lucy Williamson, Purvi Tandel, Qiu Yang, Jessica Chu, Ryan Benz, Asim Siddiqui, Daniel Hornburg, Steven Gross, Bader Shirah, Jan Krumsiek, Jaime Mateus, Xiao Mao, Irina Matei, Christopher E. Mason Secretome profiling reveals acute changes in oxidative stress, brain homeostasis, and coagulation following short-duration spaceflight Nature Communications 2024-06-11 [PMID: 38862464]
Kaitlin N. Allen, Julia María Torres-Velarde, Juan Manuel Vazquez, Diana D. Moreno-Santillán, Peter H. Sudmant, José Pablo Vázquez-Medina Hypoxia exposure blunts angiogenic signaling and upregulates the antioxidant system in endothelial cells derived from elephant seals BMC Biology 2024-04-23 [PMID: 38654271]

Jaroslav Chlupac, Jan Frank, David Sedmera, Ondrej Fabian, Zuzana Simunkova, Iveta Mrazova, Tomas Novak, Zdenka Vanourková, Oldrich Benada, Zdenek Pulda, Theodor Adla, Martin Kveton, Alena Lodererova, Ludek Voska, Jan Pirk, Jiri Fronek, Alfredo Caturano External Support of Autologous Internal Jugular Vein Grafts with FRAME Mesh in a Porcine Carotid Artery Model Biomedicines 2024-06-16 [PMID: 38927542]
Elle Koivunotko, Raili Koivuniemi, Julia Monola, Riina Harjumäki, Chris S Pridgeon, Mari Madetoja, Jere Linden, Lauri Paasonen, Saara Laitinen, Marjo Yliperttula Cellulase-assisted platelet-rich plasma release from nanofibrillated cellulose hydrogel enhances wound healing. Journal of controlled release : official journal of the Controlled Release Society 2024-04-10 [PMID: 38423475]
Amanda Wolf-Johnston, Youko Ikeda, Irina Zabbarova, Anthony J. Kanai, Sheldon Bastacky, Robert Moldwin, Joel N.H. Stern, Edwin K. Jackson, Lori A. Birder Purine nucleoside phosphorylase inhibition is an effective approach for the treatment of chemical hemorrhagic cystitis JCI Insight 2024-01-25 [PMID: 38271096]
Shishkova, D;Lobov, A;Repkin, E;Markova, V;Markova, Y;Sinitskaya, A;Sinitsky, M;Kondratiev, E;Torgunakova, E;Kutikhin, A; Calciprotein Particles Induce Cellular Compartment-Specific Proteome Alterations in Human Arterial Endothelial Cells Journal of cardiovascular development and disease 2023-12-22 [PMID: 38248875]
Dawn Bannerman, Simon Pascual-Gil, Scott Campbell, Richard Jiang, Qinghua Wu, Sargol Okhovatian, Karl T. Wagner, Miles Montgomery, Michael A. Laflamme, Locke Davenport Huyer, Milica Radisic Itaconate and citrate releasing polymer attenuates foreign body response in biofabricated cardiac patches Materials Today Bio 2023-12-18 [PMID: 38234461]
Raphael Blain, Gérard Couly, Eimad Shotar, Joséphine Blévinal, Maryne Toupin, Anais Favre, Ali Abjaghou, Megumi Inoue, Edwin Hernández-Garzón, Frédéric Clarençon, Frédéric Chalmel, Séverine Mazaud-Guittot, Paolo Giacobini, Yorick Gitton, Alain Chédotal A tridimensional atlas of the developing human head Cell 2023-12-21 [PMID: 38070509]
Kim DJ, Hahn HM, Youn YN et al. Adipose Derived Stromal Vascular Fraction and Mesenchymal Stem Cells Improve Angiogenesis in a Rat Hindlimb Ischaemia Model European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 2023-11-21 [PMID: 37995961] (IHC-P, Rat)	IHC-P	Rat
Haba D, Ohmiya T, Sekino M et al. Efficacy of wearable vibration dressings on full-thickness wound healing in a hyperglycemic rat model Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2023-11-11 [PMID: 37950849] (IHC-P, Rat)	IHC-P	Rat
Nam K, Dos Santos HT, Maslow F et al. Fibrin hydrogels fortified with FGF-7/10 and laminin-1 peptides promote regeneration of irradiated salivary glands Acta biomaterialia 2023-10-14 [PMID: 37844750] (ICC/IF, Mouse)	ICC/IF	Mouse
Jahandideh A, Virta J, Li XG et al. Vascular adhesion protein-1-targeted PET imaging in autoimmune myocarditis Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology 2023-09-27 [PMID: 37758963] (IHC, Rat)	IHC	Rat
Hu K, Liu X, Chang H et al. MicroRNA-221-3p Targets THBS1 to Promote Wound Healing in Diabetes Diabetes Metab Syndr Obes 2023-09-11 [PMID: 37720423] (IHC)	IHC
Lin PK, Koller GM, Davis GE Elucidating the Morphogenic and Signaling Roles of Defined Growth Factors Controlling Human Endothelial Cell Lumen Formation Versus Sprouting Behavior The American journal of pathology 2023-09-09 [PMID: 37689384] (WB, Human)	WB	Human
Won S, Lee C, Bae S et al. Mass-produced gram-negative bacterial outer membrane vesicles activate cancer antigen-specific stem-like CD8(+) T cells which enables an effective combination immunotherapy with anti-PD-1 Journal of Extracellular Vesicles 2023-08-10 [PMID: 37563797] (ICC/IF)	ICC/IF
Ziegler ME, Khabaz K, Khoshab N et al. Combining Allograft Adipose and Fascia Matrix as an Off-the-Shelf Scaffold for Adipose Tissue Engineering Stimulates Angiogenic Responses and Activates a Proregenerative Macrophage Profile in a Rodent Model Annals of plastic surgery 2023-08-01 [PMID: 37489973]
Allen KN, Torres-Velarde JM, Vazquez JM et al. Hypoxia blunts angiogenic signaling and upregulates the antioxidant system in elephant seal endothelial cells bioRxiv : the preprint server for biology 2023-07-03 [PMID: 37461722] (ICC/IF, Mammal) Details: Elephant seal	ICC/IF	Mammal
Abdullah S, Ghio M, Cotton-Betteridge A et al. Succinate metabolism and membrane reorganization drives the endotheliopathy and coagulopathy of traumatic hemorrhage Science advances 2023-06-16 [PMID: 37315138] (WB, Human)	WB	Human
Leong KX, Yang W, Sharma D et al. Ultrasound-stimulated microbubbles enhanced vascular disruption in fractionated radiotherapy-treated tumours via ASMase activation Disease models & mechanisms 2023-06-01 [PMID: 37278613] (IHC-P, Mouse)	IHC-P	Mouse
Jauhiainen S, Ilmonen H, Vuola P et al. ErbB signaling is a potential therapeutic target for vascular lesions with fibrous component eLife 2023-05-18 [PMID: 37199488] (IHC-Fr, Human)	IHC-Fr	Human
Li F, Liu Y, Li L et al. Brain-derived extracellular vesicles mediate traumatic brain injury associated multi-organ damage Biochemical and Biophysical Research Communications 2023-05-01 [PMID: 37163934] (ICC/IF, Mouse)	ICC/IF	Mouse
Feng G, Liu W, Yu Y et al. Angiogenesis coupled with osteogenesis in a bone tissue engineering scaffold enhances bone repair in osteoporotic bone defects Biomedical materials (Bristol, England) 2023-05-05 [PMID: 37144422] (Human)		Human
Sanchez-Moral L, Paul T, Martori C et al. Macrophage CD5L is a target for cancer immunotherapy EBioMedicine 2023-04-11 [PMID: 37054630] (IHC-P, Mouse)	IHC-P	Mouse
Qiu M, Li C, Cai Z et al. 3D Biomimetic Calcified Cartilaginous Callus that Induces Type H Vessels Formation and Osteoclastogenesis Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023-03-31 [PMID: 36999832] (IHC-P, Rat)	IHC-P	Rat
Nikoli? J, Vukojevi? K, �olji? V et al. Expression Patterns of Serotonin Receptors 5-HT1A, 5-HT2A, and 5-HT3A during Human Fetal Lung Development International journal of molecular sciences 2023-02-03 [PMID: 36769290] (IHC-P, Human) Details: Dilution used in IHC-P 1:100	IHC-P	Human
Salvador E, K�ppl T, H�rmann J et al. Tumor Treating Fields (TTFields) Induce Cell Junction Alterations in a Human 3D In Vitro Model of the Blood-Brain Barrier Pharmaceutics 2023-01-04 [PMID: 36678814] (ICC/IF)	ICC/IF
Zhao Y, Chen Z, Shao W et al. Black phosphorus-enhanced injectable hydrogel for infected soft tissue healing APL Bioengineering 2023-03-01 [PMID: 36644416] (ICC/IF)	ICC/IF
Paez-Mayorga J, Campa-Carranza JN, Capuani S et al. Implantable niche with local immunosuppression for islet allotransplantation achieves type 1 diabetes reversal in rats Nature communications 2022-12-26 [PMID: 36572684] (IHC, Rat) Details: Dilution used in IHC 1:200	IHC	Rat
Goel K, Egersdorf N, Gill A et al. Characterization of pulmonary vascular remodeling and MicroRNA-126-targets in COPD-pulmonary hypertension Respiratory research 2022-12-15 [PMID: 36522710] (IHC-P, Human) Details: Dilution used in IHC-P 1:200	IHC-P	Human
Chen Z, Wang L, Guo C et al. Vascularized polypeptide hydrogel modulates macrophage polarization for wound healing Acta biomaterialia 2022-11-15 [PMID: 36396041] (IHC-P, Mouse)	IHC-P	Mouse
Czosseck A, Chen MM, Nguyen H et al. Porous scaffold for mesenchymal cell encapsulation and exosome-based therapy of ischemic diseases Journal of controlled release : official journal of the Controlled Release Society 2022-11-14 [PMID: 36370875] (IHC-P, Mouse) Details: Dilution used in IHC-P 1:200	IHC-P	Mouse
Bonneau S, Landry C, BEgin S et al. Correlation between Neutrophil Extracellular Traps (NETs) Expression and Primary Graft Dysfunction Following Human Lung Transplantation Cells 2022-10-29 [PMID: 36359815] (ICC/IF, Human)	ICC/IF	Human
Koivunotko E, Snirvi J, Merivaara A Et al. Angiogenic Potential of Human Adipose-Derived Mesenchymal Stromal Cells in Nanofibrillated Cellulose Hydrogel Biomedicines 2022-10-27 [PMID: 36289846] (ICC/IF, Human) Details: Citation using the Azide and BSA Free version of this antibody.	ICC/IF	Human
Yamanouchi K, Tanaka Y, Ikeda M et al. Macroglossia and less advanced dystrophic change in the tongue muscle of the Duchenne muscular dystrophy rat Skeletal muscle 2022-10-19 [PMID: 36258243] (IF/IHC, Rat)	IF/IHC	Rat
Chakroborty D, Goswami S, Fan H et al. Neuropeptide Y, a paracrine factor secreted by cancer cells, is an independent regulator of angiogenesis in colon cancer British journal of cancer 2022-07-28 [PMID: 35902640] (IHC-P, IHC-Fr)	IHC-P, IHC-Fr
Thinard R, Farkas A, Halasa M et al. Endothelial antibody factory at the Blood Brain Barrier: novel approach to therapy of neurodegenerative diseases Research Square 2022-03-01 [PMID: 35890313] (ICC/IF, Mouse)	ICC/IF	Mouse
Tavé van Zyl, Wenjun Yan, Alexi M. McAdams, Aboozar Monavarfeshani, Gregory S. Hageman, Joshua R. Sanes Cell atlas of the human ocular anterior segment: Tissue-specific and shared cell types Proceedings of the National Academy of Sciences of the United States of America 2022-07-15 [PMID: 35858321]
Mylvaganam S, Plumb J, Yusuf B et al. The spectrin cytoskeleton integrates endothelial mechanoresponses Nature cell biology 2022-07-11 [PMID: 35817960] (ICC/IF, Human) Details: ICC/IF Dilution: 4.5 ug ml–1, WB Dilution: 1:1000	ICC/IF	Human
Ikeda S, Sugioka S, Kimura T, Ashida N Smooth muscle protein 22 alpha-Cre recombination in resting cardiac fibroblasts and hematopoietic precursors Scientific reports 2022-07-07 [PMID: 35798848]
Tian W, Hao H, Chu M et al. Berberine Suppresses Lung Metastasis of Cancer via Inhibiting Endothelial Transforming Growth Factor Beta Receptor 1 Frontiers in Pharmacology 2022-06-16 [PMID: 35784732] (FLOW, ICC/IF)	FLOW, ICC/IF
Verginadis II, Avgousti H, Monslow J et al. A stromal Integrated Stress Response activates perivascular cancer-associated fibroblasts to drive angiogenesis and tumour progression Nature cell biology 2022-06-01 [PMID: 35654839] (IF/IHC, Mouse)	IF/IHC	Mouse
Jassim AH, Nsiah NY, Inman DM. Ocular Hypertension Results in Hypoxia within Glia and Neurons throughout the Visual Projection Antioxidants (Basel) 2022-04-29 [PMID: 35624752] (ICC/IF)	ICC/IF
Alba GA, Samokhin AO, Wang RS et al. Pulmonary endothelial NEDD9 and the prothrombotic pathophenotype of acute respiratory distress syndrome due to SARS-CoV-2 infection Pulmonary Circulation 2022-05-11 [PMID: 35599981] (ICC/IF, IHC)	ICC/IF, IHC
Cunningham CM, Li M, Ruffenach G et al. Y-Chromosome Gene, Uty, Protects Against Pulmonary Hypertension by Reducing Proinflammatory Chemokines American journal of respiratory and critical care medicine 2022-05-03 [PMID: 35504005]
Pan Y, Cao S, Tang J et al. Cyclooxygenase-2 in adipose tissue macrophages limits adipose tissue dysfunction in obese mice The Journal of clinical investigation 2022-05-02 [PMID: 35499079] (ICC/IF, Mouse)	ICC/IF	Mouse
Hentzschel F, Gibbins MP, Attipa C et al. Host cell maturation modulates parasite invasion and sexual differentiation in Plasmodium berghei Science advances 2022-04-29 [PMID: 35476438] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Chen J, Li K, Shao J et al. Irisin Suppresses Nicotine-Mediated Atherosclerosis by Attenuating Endothelial Cell Migration, Proliferation, Cell Cycle Arrest, and Cell Senescence Frontiers in cardiovascular medicine 2022-04-08 [PMID: 35463776] (IF/IHC, Mouse)	IF/IHC	Mouse
Calvanese V, Capellera-Garcia S, Ma F et al. Mapping human haematopoietic stem cells from haemogenic endothelium to birth Nature 2022-04-01 [PMID: 35418685] (IF/IHC, Human)	IF/IHC	Human
Heiss CN, Gravert E, Hult�n M, Olofsson LE. MyD88 Deficiency, but Not Gut Microbiota Depletion, Is Sufficient to Modulate the Blood-Brain Barrier Function in the Mediobasal Hypothalamus Molecular Neurobiology 2022-06-01 [PMID: 35381888]
Nair RS, Sobhan PK, Shenoy SJ et al. A porcine cholecystic extracellular matrix conductive scaffold for cardiac tissue repair Journal of biomedical materials research. Part B, Applied biomaterials 2022-03-19 [PMID: 35305082] (IF/IHC)	IF/IHC
Xu D, Gan K, Wang y et al. A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone Repair International journal of nanomedicine 2022-03-11 [PMID: 35299865] (IHC-P, Rat)	IHC-P	Rat
Sawada J, Hiraoka N, Qi R et al. Molecular Signature of Tumor-Associated High Endothelial Venules That Can Predict Breast Cancer Survival Cancer immunology research 2022-04-01 [PMID: 35201289] (IHC-P, Human) Details: Human tonsils	IHC-P	Human
Nguyen Ho-Bouldoires TH, Sollier K, Zamfirov L et al. Ret kinase-mediated mechanical induction of colon stem cells by tumor growth pressure stimulates cancer progression in vivo Communications biology 2022-02-17 [PMID: 35177769] (IHC-Fr, Mouse)	IHC-Fr	Mouse
yang Hy, Fierro F, yoon DJ et al. Combination product of dermal matrix, preconditioned human mesenchymal stem cells and timolol promotes wound healing in the porcine wound model Journal of biomedical materials research. Part B, Applied biomaterials 2022-01-31 [PMID: 35099112]
Raj R, Shenoy S, Mony M et al. Surface Modification of Polypropylene Mesh with a Porcine Cholecystic Extracellular Matrix Hydrogel for Mitigating Host Tissue Reaction ACS Appl Bio Mater 2022-01-11 [PMID: 35014417]
Jahandideh A, StAhle M, Virta J et al. Evaluation of [68Ga]Ga-NODAGA-RGD for PET Imaging of Rat Autoimmune Myocarditis Frontiers in Medicine 2021-12-15 [PMID: 34977085] (IHC-Fr, Rat)	IHC-Fr	Rat
Yan J, Yuan P, Gui L et al. CCL28 Downregulation Attenuates Pancreatic Cancer Progression Through Tumor Cell-Intrinsic and -Extrinsic Mechanisms Technol Cancer Res Treat 2021-12-23 [PMID: 34939465] (ICC/IF)	ICC/IF
Rostami S, Xu M, Datta S, Haykal S Evaluation of Early Markers of Ischemia-reperfusion Injury and Preservation Solutions in a Modified Hindlimb Model of Vascularized Composite Allotransplantation Transplantation direct 2022-01-01 [PMID: 34912943] (IHC-P, Rat)	IHC-P	Rat
Kim H, Kim DE, Han G et al. Harnessing the Natural Healing Power of Colostrum: Bovine Milk-Derived Extracellular Vesicles from Colostrum Facilitating the Transition from Inflammation to Tissue Regeneration for Accelerating Cutaneous Wound Healing Advanced healthcare materials 2021-12-05 [PMID: 34865307] (IF/IHC, Mouse)	IF/IHC	Mouse
Liliac IM, Popescu EL, V?duva IA et al. Nanoparticle-functionalized dressings for the treatment of third-degree skin burns - histopathological and immunohistochemical study Romanian Journal of Morphology and Embryology 2021-10-10 [PMID: 34609418] (IHC)	IHC
Wang Y, Lyu Y, Tu K Et al. Histone citrullination by PADI4 is required for HIF-dependent transcriptional responses to hypoxia and tumor vascularization Science advances 2021-08-01 [PMID: 34452909] (IF/IHC, Human)	IF/IHC	Human
Taguchi E, Horiuchi K, Senoo A Et Al. Eribulin induces tumor vascular remodeling through intussusceptive angiogenesis in a sarcoma xenograft model Biochemical and biophysical research communications 2021-07-15 [PMID: 34274851] (WB)	WB
Tian J, Zhang M, Suo M et al. Dapagliflozin alleviates cardiac fibrosis through suppressing EndMT and fibroblast activation via AMPK alpha/TGF-beta/Smad signalling in type 2 diabetic rats Journal of cellular and molecular medicine 2021-06-25 [PMID: 34169635]
Alizadehgiashi M, Nemr C, Chekini M et al. Multifunctional 3D-Printed Wound Dressings ACS Nano 2021-06-16 [PMID: 34133121]
Navarro RS, Jiang L, Ouyang Y et al. Biomimetic tubular scaffold with heparin conjugation for rapid degradation in in situ regeneration of a small diameter neoartery Biomaterials 2021-05-12 [PMID: 34051629]
Li B, Chen K, Qian N et al. Baicalein alleviates osteoarthritis by protecting subchondral bone, inhibiting angiogenesis and synovial proliferation Journal of cellular and molecular medicine 2021-05-03 [PMID: 33939310] (IF/IHC, Rat)	IF/IHC	Rat
StAhle M, Hellberg S, Virta J et al. Evaluation of glucagon-like peptide-1 receptor expression in non-diabetic and diabetic atherosclerotic mice using PET tracer 68Ga-NODAGA-exendin-4 American journal of physiology. Endocrinology and metabolism 2021-04-12 [PMID: 33843281]
Hickey, R J, Modulevsky, D J Et al. Customizing the Shape and Microenvironment Biochemistry of Biocompatible Macroscopic Plant-Derived Cellulose Scaffolds. ACS Biomater Sci Eng 2018-11-12 [PMID: 33429594] (IF/IHC, Human)	IF/IHC	Human
Klak M, Kosowska K, Bryniarski T et al. Bioink based on the dECM for 3D bioprinting of bionic tissue, the first results obtained on murine model Bioprinting 2022-12-01 (IHC-P, Mouse)	IHC-P	Mouse
Dienes J, Browne S, Farjun B et al. Semisynthetic Hyaluronic Acid-Based Hydrogel Promotes Recovery of the Injured Tibialis Anterior Skeletal Muscle Form and Function ACS biomaterials science & engineering 2021-03-04 [PMID: 33660968]
Park JF, Clark VR, Banerjee S et al. Transcriptomic Analysis of Right Ventricular Remodeling in Two Rat Models of Pulmonary Hypertension: Identification and Validation of Epithelial-to-Mesenchymal Transition in Human Right Ventricular Failure Circulation. Heart failure 2021-02-02 [PMID: 33541093] (Rat)		Rat
Colzani M, Malcor J, Hunter E et al. Modulating hESC-derived cardiomyocyte and endothelial cell function with triple-helical peptides for heart tissue engineering Biomaterials 2021-02-01 [PMID: 33385684]
Tawfik A, Mohamed R, Kira D et al. N-Methyl-D-aspartate receptor activation, novel mechanism of homocysteine-induced blood-retinal barrier dysfunction J Immunol 2020-12-01 [PMID: 33159240]
Jiang D, Christ S, Correa-Gallegos D et al. Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin Nat Commun 2020-11-06 [PMID: 33159076] (ICC/IF, Human, Mouse) Details: Human and mouse scar tissue underwent immunofluorescence analysis.	ICC/IF	Human, Mouse
Ghaffari, S, Jang, E Et al. Endothelial HMGB1 Is a Critical Regulator of LDL Transcytosis via an SREBP2-SR-BI Axis. Arterioscler Thromb Vasc Biol 2021-01-01 [PMID: 33054399] (IF/IHC, Mouse)	IF/IHC	Mouse
Kutomi O, Takeda S Identification of lymphatic endothelium in cranial arachnoid granulation-like dural gap Microscopy (Oxf) 2020-07-13 [PMID: 32657336]
Das S, Browne K D et al. Pre-innervated tissue-engineered muscle promotes a pro-regenerative microenvironment following volumetric muscle loss. Commun Biol 2020-06-25 [PMID: 32587337] (IF/IHC, Rat)	IF/IHC	Rat
Liktor-Busa E, Blawn K T et al. Functional NHE1 expression is critical to blood brain barrier integrity and sumatriptan blood to brain uptake. PLoS One 2020-05-30 [PMID: 32469979] (WB, Mouse)	WB	Mouse
Gerbin KA, Mitzelfelt KA, Guan X, Martinson AM Delta-1 functionalized hydrogel promotes hESC-cardiomyocyte graft proliferation and maintains heart function post-injury Mol Ther Methods Clin Dev 2020-05-20 [PMID: 32426414]
Kitahara A, Ran Q, Oda K et al. Generation of Lungs by Blastocyst Complementation in Apneumic Fgf10-Deficient Mice Cell Rep 2020-05-12 [PMID: 32402288] (IHC-P, Mouse)	IHC-P	Mouse
Chen H, Fu H et al. Regeneration of pulpo-dentinal-like complex by a group of unique multipotent CD24a+ stem cells. Sci Adv 2020-01-04 [PMID: 32284993] (IF/IHC, Mouse)	IF/IHC	Mouse
Mensah Sa, Nersesyan Aa, Harding Ic et Al. Flow-regulated endothelial glycocalyx determines metastatic cancer cell activity FASEB J. 2020-03-13 [PMID: 32167209] (IF, Mouse)	IF	Mouse
Jiang D, Singh K, Muschhammer J et al. MSCs rescue impaired wound healing in a murine LAD1 model by adaptive responses to low TGF-beta 1 levels EMBO Rep. 2020-02-21 [PMID: 32080965] (WB, Mouse)	WB	Mouse
Li G, Wang X, Yang H et al. alpha-Linolenic acid but not linolenic acid protects against hypertension: critical role of SIRT3 and autophagic flux Cell Death Dis 2020-02-03 [PMID: 32015327] (IHC-Fr, Human)	IHC-Fr	Human
Gonzalez P, Gonzalez-Fernandez C, Campos-Martin Y et al. Spatio-temporal and Cellular Expression Patterns of PTK7 in the Healthy and Traumatically Injured Rat and Human Spinal Cord Cell. Mol. Neurobiol. 2020-01-23 [PMID: 31974907]
Lv L, Liao Z, Luo J et al. Cardiac telocytes exist in the adult Xenopus tropicalis heart J. Cell. Mol. Med. Jan 12 2020 [PMID: 31930692] (IHC, Xenopus)	IHC	Xenopus
Lv L, Liao Z, Luo J et al. Cardiac telocytes exist in the adult Xenopus tropicalis heart Journal of Cellular and Molecular Medicine 2020-02-01 [PMID: 31930692] (IHC)	IHC
Mintz EL, Passipieri JA, Franklin IR et al. Long-Term Evaluation of Functional Outcomes Following Rat Volumetric Muscle Loss Injury and Repair Tissue Eng Part A 2019-10-03 [PMID: 31578935]
Hasko J, Fazakas C, Molnar K et al. Response of the neurovascular unit to brain metastatic breast cancer cells Acta Neuropathol Commun 2019-08-19 [PMID: 31426859] (IF/IHC, Mouse)	IF/IHC	Mouse
Smith A, Thimmappa V, Boughter JD et al. The effect of intrathyroidal versus intraperitoneal bevacizumab on thyroid volume and vasculature flow in a rat model Gland Surg 2019-06-01 [PMID: 31328099] (WB, Mouse)	WB	Mouse
Ganguly E, Aljunaidy M, Kirschenman R Sex-Specific Effects of Nanoparticle-Encapsulated MitoQ (nMitoQ) Delivery to the Placenta in a Rat Model of Fetal Hypoxia Front. Physiol. 2019-05-24 [PMID: 31178743] (ICC/IF, Rat)	ICC/IF	Rat
Wang R, Lou X, Feng G et al. IL-17A-stimulated endothelial fatty acid beta-oxidation promotes tumor angiogenesis Life Sci. 2019-07-15 [PMID: 31085243] (IHC-P, Mouse)	IHC-P	Mouse
Passipieri JA, Hu X, Mintz E et al. In Silico and In Vivo Studies Detect Functional Repair Mechanisms in a Volumetric Muscle Loss Injury Tissue Eng Part A 2019-03-18 [PMID: 30882277] (IHC-P, Rat)	IHC-P	Rat
Negi H, Merugu SB, Mangukiya HB et al. Anterior Gradient-2 Monoclonal Antibody Inhibits Lung Cancer Growth and Metastasis by Upregulating p53 Pathway and Without Exerting Any Toxicological Effects: A Preclinical Study. Cancer Lett. 2019-01-24 [PMID: 30685412] (IHC-P, Mouse)	IHC-P	Mouse
Yan Y, Chen H, Zhang H et al. Vascularized 3D printed scaffolds for promoting bone regeneration. Biomaterials. 2018-10-31 [PMID: 30415019] (IF/IHC, Rat)	IF/IHC	Rat
Suen CM, Chaudhary KR, Deng Y et al. Fischer rats exhibit maladaptive structural and molecular right ventricular remodelling in severe pulmonary hypertension: a genetically prone model for right heart failure Cardiovasc. Res. 2018-10-24 [PMID: 30357319] (IF/IHC, Rat)	IF/IHC	Rat
Chen H, Cheng R, Zhao X et al. An injectable self-healing coordinative hydrogel with antibacterial and angiogenic properties for diabetic skin wound repair. NPG Asia Material 1905-07-11 [PMID: 30241849] (IF/IHC, Rat)	IF/IHC	Rat
Heikal L, Ghezzi P, Mengozzi M, Ferns G Assessment of HIF-1a expression and release following endothelial injury in-vitro and in-vivo. Mol Med 2018-05-16 [PMID: 30134815]
Miura Y, Matsui S, Miyata N et al. Differential expression of Lutheran/BCAM regulates biliary tissue remodeling in ductular reaction during liver regeneration Elife 2018-07-30 [PMID: 30059007] (ICC/IF, Mouse)	ICC/IF	Mouse
Huesing CJ Anatomical Organization and Distinction of the Sympathetic Inputs to Interscapular Brown Adipose Tissue and Inguinal White Adipose Tissue in the Mouse Thesis 2020-01-01 (IF/IHC, Mouse)	IF/IHC	Mouse
Cheng L, Cai Z, Ye T et al. Injectable Polypeptide Protein Hydrogels for Promoting Infected Wound Healing Adv. Funct. Mater. 2020-05-04
Brevini T Developing novel prophylactic approaches against SARS-CoV-2 infection Thesis 2023-01-01 (ICC/IF, Human) Details: 1:100 ICC/IF dilution	ICC/IF	Human
Follin B, Ghotbi AA, Clemmensen AE et al. Retention and Functional Effect of Adipose-Derived Stromal Cells Administered in Alginate Hydrogel in a Rat Model of Acute Myocardial Infarction. Stem Cells Int 2018-03-26 [PMID: 29765421]
Serra Monica, Marongiu Michela, Contini Antonella et al. Evidence of Amniotic Epithelial Cell Differentiation toward Hepatic Sinusoidal Endothelial Cells. Cell Transplantation 2018-03-22 [PMID: 29562778] (ICC/IF, IF/IHC, Rat)	ICC/IF, IF/IHC	Rat
Ribot Emeline J, Tournier Clement, Aid-Launais Rachida et al. 3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneRation of femoral bone defect in Rats. Scientific Reports 2017-01-01 [PMID: 28733632] (IF/IHC, Rat)	IF/IHC	Rat
El-Rabahi T EXPLORING THE SUSCEPTIBILITY TO CARDIAC ARRHYTHMIAS AND UNDERLYING MECHANISMS IN PULMONARY ARTERIAL HYPERTENSION Thesis 2023-01-01 (IHC-P, Rat) Details: Dilution: 1:100	IHC-P	Rat
Cheng G, Chang FJ, You PH et al. ZIP8 induces monocyte adhesion to the aortas ex-vivo by regulating zinc influx. Int. Immunopharmacol. 2018-07-13 [PMID: 30015240] (WB, Mouse)	WB	Mouse
Jiang L, Zhang W, Wei L et al. Early effects of parathyroid hormone on vascularized bone regeneration and implant osseointegration in aged rats. Biomaterials. 2018-10-01 [PMID: 29960821] (ICC/IF, Rat)	ICC/IF	Rat
Da Silva Filho J, Herder V, Gibbins M et al. Disease trajectories in hospitalized COVID-19 patients are predicted by clinical and peripheral blood signatures representing distinct lung pathologies medRxiv 2023-09-09 (IHC-P, Human) Details: 2 ug/mL dilution	IHC-P	Human
Bhave S, Esposito M, Swain L et al. Loss of Bone Morphogenetic Protein (BMP)-9 Reduces Survival and Increases MMP Activity after Myocardial Infarction JACC: Basic to Translational Science 2023-08-01 (WB, Mouse)	WB	Mouse
Mohamed R, Sharma I, Ibrahim AS et al. Hyperhomocysteinemia Alters Retinal Endothelial Cells Barrier Function and Angiogenic Potential via Activation of Oxidative Stress Sci Rep 2017-09-20 [PMID: 28931831] (ICC/IF, Human)	ICC/IF	Human
Ehret C, Aid-Launais R, Sagardoy T et al. Strontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation PLoS ONE 2017-09-14 [PMID: 28910401] (IHC-P, Human)	IHC-P	Human
Çavdar S, Köse B, Alt?nöz D et al. Lymphatic Vessels Accompanying Dorsal and Basal Dural Sinuses in the Human Brain Journal of Chemical Neuroanatomy 2023-10-01
Meyers MW, Rink JS, Jiang Q et al. Systemically administered collagen-targeted gold nanoparticles bind to arterial injury following vascular interventions. Physiol Rep. 2017-02-01 [PMID: 28242820] (ICC/IF)	ICC/IF
Oliveira H, Catros S, Castano O et al. The proangiogenic potential of a novel calcium releasing composite biomaterial: orthotopic in vivo evaluation. Acta Biomater. 2017-02-24 [PMID: 28242456] (WB, Rat)	WB	Rat
Luo L, Chen B, Huang Y et al. Cardioprotective activity of placental growth factor combined with oral supplementation of l-arginine in a rat model of acute myocardial infarction. Drug Des Devel Ther. 2016-11-08 [PMID: 27822012] (Rat)		Rat
Moon J, Kim Jh, Choi D et al. Correlation of quantitative dynamic contrast-enhanced MRI with microvascular density in necrotic, partial necrotic, and viable liver tumors in a rabbit model. J Appl Clin Med Phys 2016-09-30 [PMID: 27685133] (IF/IHC)	IF/IHC
Oliveira H, Catros S, Boiziau C et al. The proangiogenic potential of a novel calcium releasing biomaterial: Impact on cell recruitment Acta Biomaterialia 2016-01-01 [PMID: 26441126] (IHC)	IHC
Gerbin KA, Mitzelfelt KA, Guan X, Martinson AM Delta-1 functionalized hydrogel promotes hESC-cardiomyocyte graft proliferation and maintains heart function post-injury bioRxiv (IF/IHC)	IF/IHC
Modulevsky DJ, Cuerrier CM, Pelling AE. Biocompatibility of Subcutaneously Implanted Plant-Derived Cellulose Biomaterials. PLoS One 2016-06-21 [PMID: 27328066] (ICC/IF)	ICC/IF
De Paoli F, Eeckhoute J, Copin C et al. The neuron-derived orphan receptor 1 (NOR1) is induced upon human alternative macrophage polarization and stimulates the expression of markers of the M2 phenotype Atherosclerosis 2015-01-01 [PMID: 25941992] (IHC-Fr, Human)	IHC-Fr	Human
Bories G, Colin S, Vanhoutte J et al. Liver X Receptor (LXR) Activation Stimulates Iron Export in Human Alternative Macrophages. Circ Res. 2013-09-13 [PMID: 24036496] (IF/IHC, Human)	IF/IHC	Human
Hylander BL, Punt N, Tang H et al. Origin of the vasculature supporting growth of primary patient tumor xenografts. J Transl Med 2013-05-03 [PMID: 23639003] (IHC-P, Human)	IHC-P	Human
Jiang D, Qi Y, Walker NG et al. The effect of adipose tissue derived MSCs delivered by a chemically defined carrier on full-thickness cutaneous wound healing. Biomaterials 2013-03-01 [PMID: 23317813] (WB, IHC-Fr, ICC/IF, Mouse)	WB, IHC-Fr, ICC/IF	Mouse
Abele-Ohl S, Leis M, Wollin M et al. Human cytomegalovirus infection leads to elevated levels of transplant arteriosclerosis in a humanized mouse aortic xenograft model. Am J Transplant 2012-07-01 [PMID: 22429329]
Romon Rodrigue, Adriaenssens Eric, Lagadec Chann et al. Nerve growth factor promotes breast cancer angiogenesis by activating multiple pathways. Molecular Cancer 2010-01-01 [PMID: 20569463] (IF/IHC, Mouse)	IF/IHC	Mouse
Laga AC, Lai CY, Zhan Q et al. Expression of the embryonic stem cell transcription factor SOX2 in human skin: relevance to melanocyte and merkel cell biology. Am J Pathol. 2010-02-01 [PMID: 20042675] Details: IHC (paraffin): Labeled with SOX2 antibody, results described on page 908.
Ackler S, Xiao Y, Mitten MJ et al. ABT-263 and rapamycin act cooperatively to kill lymphoma cells in vitro and in vivo. Mol Cancer Ther. 2008-10-01 [PMID: 18852130]
Patten PEM, Buggins AGS, Richards J et al. CD38 expression in chronic lymphocytic leukemia is regulated by the tumor microenvironment. Blood;111(10):5173-81. 2008-05-15 [PMID: 18326821]
Schatton T, Murphy GF, Frank NY et al. Identification of cells initiating human melanomas. Nature 2008-01-01 [PMID: 18202660]
Wang F, Li SS, Segersvard R et al. Hypoxia inducible factor-1 mediates effects of insulin on pancreatic cancer cells and disturbs host energy homeostasis. Am J Pathol 2007-02-01 [PMID: 17255315]
Yang, Q-C, Zeng, B-F, Dong, Y, Shi, Z-M, Jiang, Z-M, Huang, J. Overexpression of Hypoxia-Inducible Factor-1{alpha} in Human Osteosarcoma: Correlation with Clinicopathological Parameters Survival Outcome, . 37, 127-134. 2007-01-01 [PMID: 17237146]
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Reviews for CD31/PECAM-1 Antibody (NB100-2284) (6) 4.76

Average Rating: 4.7

(Based on 6 reviews)

We have 6 reviews tested in 5 species: Human, Mouse, Rat, Canine, Other.

Reviews using NB100-2284:

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IHC-P

(3)

IHC-Fr

(1)

ICC

(2)

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Human

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Mouse

(1)

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reviewed by:
Hannah Wickham

IHC-P

Canine

04/28/2022

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Summary

Application	Immunohistochemistry-Paraffin
Sample Tested	Adult kidney
Species	Canine
Lot	2

Comments

Antibody was used at a 1:50 concentration in Casein in PBS and left at 4C overnight on paraffin embedded canine bladder tissue. HIER was performed in Tris/EDTA buffer, pH 9 for two hours at 75C.

Immunohistochemistry-Frozen CD31/PECAM-1 NB100-2284

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reviewed by:
Verified Customer

IHC-Fr

Mouse

01/07/2021

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Summary

Application	Immunohistochemistry-Frozen
Sample Tested	E11.5 mouse embryo fixed in 4% PFA
Species	Mouse
Lot	N/A

Comments

Fixed 4% PFA overnight.
Blocked with 1% BSA
Primary antibody dilution - 1:100
Secondary antibody - Invitrogen Alexa Fluor 488
Secondary antibody dilution - 1:1000

Immunohistochemistry-Paraffin CD31/PECAM-1 NB100-2284

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reviewed by:
David Ivancic

IHC-P

Rat

02/01/2018

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Summary

Application	Immunohistochemistry-Paraffin
Sample Tested	Rat liver
Species	Rat
Lot	2

Comments

Formalin fixed Paraffin embedded rat liver tissue
Antigen retrieval pH6.0 citrate buffer.
Sea Block blocking solution 30min.
NB100-2284 diluted 1/100 in Sea Block and incubated overnight at 4degC
Alexa Fluor® 488 Goat anti Rabbit 1/300 in Sea Block for 1hr at 37degC.
Antifade blocking mounting media with DAPI
Axio Observer 7 Apotome Imaging 40x

Immunocytochemistry CD31/PECAM-1 NB100-2284

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reviewed by:
Fabio Marongiu

ICC

Human

08/29/2017

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Summary

Application	Immunocytochemistry
Sample Tested	HUVEC (Human Umbilical Vein Endothelial Cells)
Species	Human
Lot	2

Comments

Fixation 4% PFA
Blocking Goat Serum
Dilution 1:100
Incubation 2h @ RT

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reviewed by:
Verified Customer

ICC

Other

01/07/2016

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Summary

Application	Immunocytochemistry
Sample Tested	SC cells
Species	Other

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reviewed by:
Naiche Adler

IHC-P

Rat

01/27/2015

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Summary

Application	Immunohistochemistry-Paraffin
Sample Tested	rat muscle FFPE
Species	Rat

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FAQs for CD31/PECAM-1 Antibody (NB100-2284). (Showing 1 - 3 of 3 FAQs).

I'm interested in your antibody CD31/PECAM1 ref: NB100-2284. I'd like to stain human endothelial cells injected in mouse muscle. Do you know if your antibody cross-reacts with mouse? Is this antibody working on Immunohistochemistry frozen sections?
- NB100-2284 reacts with human and mouse in WB, immunocytochemistry and IHC on both frozen and paraffin-embedded tissues. Novus also offers sample sizes of several products, which may be identified by a SS at the end of the catalog number (ie. NB100-105SS is the sample size of NB100-105). We also offer Super Novus packs to many common antigens. These kits enable customers to test several different antibodies to the target at a discounted rate.
I'm interested in your antibody CD31/PECAM1 ref: NB100-2284. I'd like to stain human endothelial cells injected in mouse muscle. Do you know if your antibody cross-reacts with mouse? Is this antibody working on Immunohistochemistry frozen sections?
- As is listed on our datasheet, NB100-2284 reacts with human and mouse in WB, immunocytochemistry and IHC on both frozen and paraffin-embedded tissues. Novus also offers sample sizes of several products, which may be identified by a "SS" at the end of the catalog number (ie. NB100-105SS is the sample size of NB100-105). We also offer Super Novus packs to many common antigens. These kits enable customers to test several different antibodies to the target at a discounted rate.
I am looking for references concerning the use of NBP2-15797, NBP1-47913 and NB100-2284 in IHC on PFA- fixed section.
- Among your mentioned catalog numbers (NBP2-15797, NBP1-47913 and NB100-2284), we have published references for NB100-2284 only. Hylander et al. 2013 (Journal of Translational Medicine 2013, 11:110) have cited the IHC-P use of NB100-2284 and this paper has also explained a nice detailed IHC-P protocol that you may follow if you want to. NBP1-71663 is our bestselling catalog number among all CD31/PECAM1 antibodies and it has been cited in two publications, both explaining its use in IHC-P assays (Pratheeshkumar et al. 2012 PLoS One. 2012;7(10):e47516; and Pratheeshkumar et al. 2012 PLoS One. 2012;7(12):e52279)

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Research Areas for CD31/PECAM-1 Antibody (NB100-2284)

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The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells. Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol... Read full blog post.

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Recent Reviews

4.7

5	4
4	2
3	0
2	0
1	0

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	Hannah Wickham 04/28/2022
Application:	IHC-P
Species:	Canine

read this review

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	Verified Customer 01/07/2021
Application:	IHC-Fr
Species:	Mouse

read this review

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	David Ivancic 02/01/2018
Application:	IHC-P
Species:	Rat

read this review

Bioinformatics

Gene Symbol	PECAM1
Entrez	5175(Human)
Uniprot	P16284()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

CD31/PECAM-1 Antibody

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