CD31/PECAM-1 Antibody [Unconjugated]

Name: CD31/PECAM-1 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF806
Rating: 5 (1 reviews)

(71 Publications)

(1 Review)

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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) ...read more

CD31/PECAM-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Sheep Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) for 3 hours at room ...read more

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD31/PECAM-1 at ...read more

CD31/PECAM‑1 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line but is not detected in CD31/PECAM‑1 knockout (KO) THP‑1 human cell line using Sheep Anti-Human ...read more

CD31/PECAM‑1 was detected in immersion fixed paraffin-embedded sections of human liver using Sheep Anti-Human CD31/PECAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF806) at ...read more

Expression pattern of integrin receptors and type IV collagen in normal pancreatic tissue. A. H&E staining of normal exocrine pancreas with acini structures and a duct in the insert. The area shown is representative for ...read more

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB, Simple Western, IHC, ICC/IF, KO
Clonality	Polyclonal
Host	Sheep
Conjugate	Unconjugated
Concentration	LYOPH

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 488 FAB806G-100

Biotin BAF806

View Available Formulations

Catalog# & Formulation	Size	Price
Carrier Free AF806-SP

Carrier Free FAB806G-100

Novus Biologicals offers 100% Guarantee

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 488	FAB806G-100
Biotin	BAF806

CD31/PECAM-1 Antibody [Unconjugated] Summary

Immunogen	Chinese hamster ovary cell line CHO-derived recombinant human CD31 Extracellular domain
Specificity	Detects human CD31 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD31 is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Sheep
Gene	PECAM1
Purity Statement	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Immunocytochemistry 5-15 ug/mL
Immunohistochemistry 5-25 ug/mL
Knockout Validated 5-15 ug/mL
Simple Western 10 ug/mL
Western Blot 1 ug/mL

Reviewed Applications

Read 1 Review rated 5

using
AF806 in the following applications:

Immunohistochemistry-Frozen

Publications

Read Publications using
AF806 in the following applications:

FACS
1 publication
Flourescense Microscopy
1 publication
Flow Cytometry
1 publication
ICC
16 publications
IHC
13 publications
IHC-Fr
5 publications
IHC-P
4 publications
Immunocytochemistry
3 publications
Immunohistochemistry
6 publications
Western Blot
2 publications

Packaging, Storage & Formulations

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD31/PECAM-1 Antibody [Unconjugated]

adhesion molecule
CD31 antigen
CD31
CD31/EndoCAM
EndoCAM
FLJ34100
FLJ58394
GPIIA'
PECA1
PECAM1
PECAM-1
PECAM-1, CD31/EndoCAM
platelet endothelial cell adhesion molecule
platelet endothelial cell adhesion molecule-1
platelet/endothelial cell adhesion molecule

Background

The CD31 adhesion molecule, also known as PECAM-1, is expressed in large amounts on endothelial cells at intercellular junctions and on T cell subsets, and to a lesser extent on platelets and most other leukocytes such as monocytes and neutrophils. CD31 binds to itself homotypically, and also to the leukocyte integrin alpha v beta 3 heterotypically. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells. CD31 has been found in human plasma, and the presence of this circulating isoform is suggested to modulate the transendothelial migration of leukocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (AF806)(71)

We have publications tested in 7 confirmed species: Human, Mouse, Bovine, Primate, Primate - Macaca fuscata (Japanese Macaque), Primate - Macaca mulatta (Rhesus Macaque), Xenograft.

We have publications tested in 10 applications: FACS, Flourescense Microscopy, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunocytochemistry, Immunohistochemistry, Western Blot.

Showing Publications 1 - 10 of 71. Show All 71 Publications.

Publications using AF806	Applications	Species
Pan, Z;Yao, Q;Kong, W;Ma, X;Tian, L;Zhao, Y;Zhu, S;Chen, S;Sun, M;Liu, J;Jiang, S;Ma, J;Liu, Q;Peng, X;Li, X;Hong, Z;Hong, Y;Wang, X;Liu, J;Zhang, J;Zhang, W;Sun, B;Pahlavan, S;Xia, Y;Shen, W;Liu, Y;Jiang, W;Xie, Z;Kong, W;Wang, X;Wang, K; Generation of iPSC-derived human venous endothelial cells for the modeling of vascular malformations and drug discovery Cell stem cell 2024-11-22 [PMID: 39579761] (FACS, Mouse)	FACS	Mouse
Yao, K;Yang, M;Shu, M;Wang, T;Gao, D;Zhou, L;Wang, G;Zhang, Z;Tang, J; SOX4 promotes vascular abnormality in glioblastoma and is a novel target to improve drug delivery Translational oncology 2024-09-16 [PMID: 39288695] (Immunohistochemistry, Mouse)	Immunohistochemistry	Mouse
Bhattacharya, R;Ward, T;Kalejaiye, TD;Mishra, A;Leeman, S;Arzaghi, H;Seidman, JG;Seidman, CE;Musah, S; Engineered human iPS cell models reveal altered podocytogenesis and glomerular capillary wall in CHD-associated SMAD2 mutations bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39211233] (Immunocytochemistry, Human)	Immunocytochemistry	Human
Yang, L;Han, Y;Zhang, T;Dong, X;Ge, J;Roy, A;Zhu, J;Lu, T;Vandana, JJ;de Silva, N;Robertson, CC;Xiang, JZ;Pan, C;Sun, Y;Que, J;Evans, T;Liu, C;Wang, W;Naji, A;Parker, SCJ;Schwartz, RE;Chen, S; Human Vascularized Macrophage-Islet Organoids to Model Immune-Mediated Pancreatic ? cell Pyroptosis upon Viral Infection bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39149298] (Immunocytochemistry, Human)	Immunocytochemistry	Human
Li, B;Xiong, W;Zuo, W;Shi, Y;Wang, T;Chang, L;Wu, Y;Ma, H;Bian, Q;Chang, ACY; Proximal telomeric decompaction due to telomere shortening drives FOXC1-dependent myocardial senescence Nucleic acids research 2024-04-18 [PMID: 38634789] (Immunohistochemistry, Human)	Immunohistochemistry	Human
Zhang, L;Yin, Z;Liu, X;Jin, G;Wang, Y;He, L;Li, M;Pang, X;Yan, B;Jia, Z;Ma, J;Wei, J;Cheng, F;Li, D;Wang, L;Han, Z;Liu, Q;Chen, F;Cao, H;Lei, P; Dietary emulsifier polysorbate 80 exposure accelerates age-related cognitive decline Brain, behavior, and immunity 2024-03-31 [PMID: 38565398] (Immunohistochemistry, Mouse)	Immunohistochemistry	Mouse
Murdock, MH;Yang, CY;Sun, N;Pao, PC;Blanco-Duque, C;Kahn, MC;Kim, T;Lavoie, NS;Victor, MB;Islam, MR;Galiana, F;Leary, N;Wang, S;Bubnys, A;Ma, E;Akay, LA;Sneve, M;Qian, Y;Lai, C;McCarthy, MM;Kopell, N;Kellis, M;Piatkevich, KD;Boyden, ES;Tsai, LH; Multisensory gamma stimulation promotes glymphatic clearance of amyloid Nature 2024-02-28 [PMID: 38418876] (Immunohistochemistry, Human)	Immunohistochemistry	Human
Raval, AN;Schmuck, EG;Roy, S;Saito, Y;Zhou, T;Conklin, J;Hacker, TA;Koonce, C;Boyer, M;Stack, K;Hebron, E;Nagle, SK;Hsieh, PCH;Kamp, TJ; Human iPSC-derived Committed Cardiac Progenitors Generate Cardiac Tissue Grafts in a Swine Ischemic Cardiomyopathy Model without Triggering Ventricular Arrhythmias bioRxiv : the preprint server for biology 2024-02-17 [PMID: 38405927] (Immunocytochemistry, Human)	Immunocytochemistry	Human
Frenz-Wiessner, S;Fairley, SD;Buser, M;Goek, I;Salewskij, K;Jonsson, G;Illig, D;Zu Putlitz, B;Petersheim, D;Li, Y;Chen, PH;Kalauz, M;Conca, R;Sterr, M;Geuder, J;Mizoguchi, Y;Megens, RTA;Linder, MI;Kotlarz, D;Rudelius, M;Penninger, JM;Marr, C;Klein, C; Generation of complex bone marrow organoids from human induced pluripotent stem cells Nature methods 2024-02-19 [PMID: 38374263] (Immunohistochemistry, Human, Xenograft)	Immunohistochemistry	Human, Xenograft
Shi, G;Liu, L;Cao, Y;Ma, G;Zhu, Y;Xu, J;Zhang, X;Li, T;Mi, L;Jia, H;Zhang, Y;Liu, X;Zhou, Y;Li, S;Yang, G;Liu, X;Chen, F;Wang, B;Deng, Q;Zhang, S;Zhang, J; Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1?/ASK1/JNK signaling pathway in mice with traumatic brain injury Journal of neuroinflammation 2023-09-30 [PMID: 37777772] (IHC, Mouse)	IHC	Mouse
Y Gu, Y Zhou, S Ju, X Liu, Z Zhang, J Guo, J Gao, J Zang, H Sun, Q Chen, J Wang, J Xu, Y Xu, Y Chen, Y Guo, J Dai, H Ma, C Wang, G Jin, C Li, Y Xia, H Shen, Y Yang, X Guo, Z Hu Multi-omics profiling visualizes dynamics of cardiac development and functions Cell Reports, 2022-12-27;41(13):111891. 2022-12-27 [PMID: 36577384] (Mouse)		Mouse
JKE Spetz, MHC Florido, CS Fraser, X Qin, J Choiniere, SJ Yu, R Singh, M Friesen, LL Rubin, JE Salem, JJ Moslehi, KA Sarosiek Heightened apoptotic priming of vascular cells across tissues and life span predisposes them to cancer therapy-induced toxicities Science Advances, 2022-11-09;8(45):eabn6579. 2022-11-09 [PMID: 36351019] (ICC, IHC, Human, Mouse)	ICC, IHC	Human, Mouse
Shin HS, Thakore A, Tada Y et al. Angiogenic stem cell delivery platform to augment post-infarction neovasculature and reverse ventricular remodeling Scientific Reports 2022-10-20 [PMID: 36266453]
Qi Zhang, Chang Liu, Rubing Shi, Shiyi Zhou, Huimin Shan, Lidong Deng et al. Blocking C3d+/GFAP+ A1 Astrocyte Conversion with Semaglutide Attenuates Blood-Brain Barrier Disruption in Mice after Ischemic Stroke Aging and disease 2022-06-01 [PMID: 35656116]
Kelvin Yin, Daniel Patten, Sarah Gough, Susana de Barros Gonçalves, Adelyne Chan, Ioana Olan et al. Senescence-induced endothelial phenotypes underpin immune-mediated senescence surveillance Genes & Development 2022-05-01 [PMID: 35618311]
Mai T. Ngo, Victoria R. Barnhouse, Aidan E. Gilchrist, Bhushan P. Mahadik, Christine J. Hunter, Joy N. Hensold et al. Hydrogels Containing Gradients in Vascular Density Reveal Dose‐Dependent Role of Angiocrine Cues on Stem Cell Behavior Advanced Functional Materials 2021-12-16 [PMID: 35558090]
Lissenya B. Argueta, Lauretta A. Lacko, Yaron Bram, Takuya Tada, Lucia Carrau, André Figueiredo Rendeiro et al. Inflammatory responses in the placenta upon SARS-CoV-2 infection late in pregnancy iScience 2022-05-20 [PMID: 35434541]
Alexandra R. Harris, Savieay Esparza, Mohammad S. Azimi, Robert Cornelison, Francesca N. Azar, Danielle C. Llaneza et al. Platinum Chemotherapy Induces Lymphangiogenesis in Cancerous and Healthy Tissues That Can be Prevented With Adjuvant Anti-VEGFR3 Therapy Frontiers in Oncology 2022-03-17 [PMID: 35372032]
Kate A. Wickham, Line B. Nørregaard, Mikkel Oxfeldt, Stephen S. Cheung, Lasse Gliemann, Mette Hansen et al. Short-Term Supplementation With Fermented Red Clover Extract Reduces Vascular Inflammation in Early Post-menopausal Women Frontiers in Cardiovascular Medicine 2022-02-10 [PMID: 35224058]
Elizabeth S. Taglauer, Elisha M. Wachman, Lillian Juttukonda, Timothy Klouda, Jiwon Kim, Qiong Wang et al. Acute Severe Acute Respiratory Syndrome Coronavirus 2 Infection in Pregnancy Is Associated with Placental Angiotensin-Converting Enzyme 2 Shedding The American Journal of Pathology 2023-04-01 [PMID: 35090860]
Amita Datta-Mannan, Robin Brown, Stephanie Key, Paul Cain, Yiqing Feng Pharmacokinetic Developability and Disposition Profiles of Bispecific Antibodies: A Case Study with Two Molecules Antibodies (Basel) 2021-12-28 [PMID: 35076469]
G Jo, J Bae, HJ Hong, AR Han, DK Kim, SP Hong, JA Kim, S Lee, GY Koh, HM Kim Structural insights into the clustering and activation of Tie2 receptor mediated by Tie2 agonistic antibody Nature Communications, 2021-11-01;12(1):6287. 11/1/2021 [PMID: 34725372]
Timothy Klouda, Yuan Hao, Hyunbum Kim, Jiwon Kim, Judith Olejnik, Adam J. Hume et al. Interferon-alpha or -beta facilitates SARS-CoV-2 pulmonary vascular infection by inducing ACE2 Angiogenesis 2022-05-01 [PMID: 34714440]
Y Roye, R Bhattachar, X Mou, Y Zhou, MA Burt, S Musah A Personalized Glomerulus Chip Engineered from Stem Cell-Derived Epithelium and Vascular Endothelium Micromachines, 2021-08-16;12(8):. 2021-08-16 [PMID: 34442589] (ICC, Human)	ICC	Human
Lyu Z, Park J, Kim KM Et al. A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke Nature biomedical engineering 8/1/2021 [PMID: 34385693]
Y Xie, L He, R Lugano, Y Zhang, H Cao, Q He, M Chao, B Liu, Q Cao, J Wang, Y Jiao, Y Hu, L Han, Y Zhang, H Huang, L Uhrbom, C Betsholtz, L Wang, A Dimberg, L Zhang Key molecular alterations in endothelial cells in human glioblastoma uncovered through single-cell RNA sequencing JCI Insight, 2021-08-09;0(0):. 2021-08-09 [PMID: 34228647] (IHC, Human)	IHC	Human
LB Argueta, LA Lacko, Y Bram, T Tada, L Carrau, T Zhang, S Uhl, BC Lubor, V Chandar, C Gil, W Zhang, B Dodson, J Bastiaans, M Prabhu, CM Salvatore, YJ Yang, RN Baergen, BR tenOever, NR Landau, S Chen, RE Schwartz, H Stuhlmann SARS-CoV-2 Infects Syncytiotrophoblast and Activates Inflammatory Responses in the Placenta bioRxiv : the preprint server for biology, 2021-06-17;0(0):. 2021-06-17 [PMID: 34100019] (ICC, Human)	ICC	Human
YG Gete, LW Koblan, X Mao, M Trappio, B Mahadik, JP Fisher, DR Liu, K Cao Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS Aging Cell, 2021-06-04;0(0):e13388. 2021-06-04 [PMID: 34086398] (IHC, Western Blot, Human)	IHC, Western Blot	Human
Tang X, Uhl S, Zhang T et al. SARS-CoV-2 infection induces beta cell transdifferentiation Cell Metabolism 5/1/2021 [PMID: 34081913]
Fabien Kawecki, Todd Galbraith, William P. Clafshenkel, Michel Fortin, François A. Auger, Julie Fradette In Vitro Prevascularization of Self-Assembled Human Bone-Like Tissues and Preclinical Assessment Using a Rat Calvarial Bone Defect Model Materials (Basel) 2021-04-17 [PMID: 33920607]
KV Manian, CA Galloway, S Dalvi, AA Emanuel, JA Mereness, W Black, L Winschel, C Soto, Y Li, Y Song, W DeMaria, A Kumar, I Slukvin, MP Schwartz, WL Murphy, B Anand-Apte, M Chung, DSW Benoit, R Singh 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration Cell Stem Cell, 2021-03-29;0(0):. 2021-03-29 [PMID: 33784497] (ICC, Human)	ICC	Human
G Campostrin, V Meraviglia, E Giacomelli, RWJ van Helden, L Yiangou, RP Davis, M Bellin, VV Orlova, CL Mummery Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells Nature Protocols, 2021-03-26;0(0):. 2021-03-26 [PMID: 33772245] (Flow Cytometry, ICC, Human)	Flow Cytometry, ICC	Human
EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development Cell Stem Cell, 2020-09-17;0(0):. 2020-09-17 [PMID: 32946788] (IHC, Human)	IHC	Human
CJ Mietus, TJ Lackner, PS Karvelis, GT Willcockso, CM Shields, NG Lambert, P Koutakis, MA Fuglestad, H Hernandez, GR Haynatzki, JKS Kim, HK DeSpiegela, II Pipinos, GP Casale Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia J Clin Med, 2020-08-08;9(8):. 2020-08-08 [PMID: 32784470] (ICC, IHC, Bovine, Human)	ICC, IHC	Bovine, Human
Abidemi Junaid, Johannes Schoeman, Wei Yang, Wendy Stam, Alireza Mashaghi, Anton Jan van Zonneveld et al. Metabolic response of blood vessels to TNF alpha eLife 2020-08-04 [PMID: 32749215]
L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell, 2020-06-19;27(1):125-136.e7. 2020-06-19 [PMID: 32579880] (ICC, IHC, Human)	ICC, IHC	Human
L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell, 2020;27(1):125-136.e7. 2020 [PMID: 32579880] (IHC, Human)	IHC	Human
C Caneparo, C Baratange, S Chabaud, S Bolduc Conditioned medium produced by fibroblasts cultured in low oxygen pressure allows the formation of highly structured capillary-like networks in fibrin gels Sci Rep, 2020-06-09;10(1):9291. 2020-06-09 [PMID: 32518266] (ICC, Human)	ICC	Human
JW Blanchard, M Bula, J Davila-Vel, LA Akay, L Zhu, A Frank, MB Victor, JM Bonner, H Mathys, YT Lin, T Ko, DA Bennett, HP Cam, M Kellis, LH Tsai Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes Nat. Med., 2020-06-08;26(6):952-963. 2020-06-08 [PMID: 32514169] (ICC, Human)	ICC	Human
E Giacomelli, V Meraviglia, G Campostrin, A Cochrane, X Cao, RWJ van Helden, A Krotenberg, M Mircea, S Kostidis, RP Davis, BJ van Meer, CR Jost, AJ Koster, H Mei, DG Míguez, AA Mulder, M Ledesma-Te, G Pompilio, L Sala, DCF Salvatori, RC Slieker, E Sommariva, AAF de Vries, M Giera, S Semrau, LGJ Tertoolen, VV Orlova, M Bellin, CL Mummery Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease Cell Stem Cell, 2020-05-26;26(6):862-879.e11. 2020-05-26 [PMID: 32459996] (IHC, Human)	IHC	Human
Dongdong Zhang, Andrew J. Kinloch, Abhinav Srinath, Robert Shenkar, Romuald Girard, Rhonda Lightle et al. Antibodies in cerebral cavernous malformations react with cytoskeleton autoantigens in the lesional milieu Journal of Autoimmunity 2020-09-01 [PMID: 32362501]
Oleg Varlamov, Matthew Bucher, Leslie Myatt, Natali Newman, Kathleen A. Grant Daily Ethanol Drinking Followed by an Abstinence Period Impairs Bone Marrow Niche and Mitochondrial Function of Hematopoietic Stem/Progenitor Cells in Rhesus Macaques Alcoholism: Clinical and Experimental Research 2020-05-01 [PMID: 32220015]
Jacob J. Robino, Nathalie Pamir, Sara Rosario, Lindsey B. Crawford, Benjamin J. Burwitz, Charles T. Roberts et al. Spatial and biochemical interactions between bone marrow adipose tissue and hematopoietic stem and progenitor cells in rhesus macaques Bone 2020-04-01 [PMID: 31972314]
HE Weiss-Bilk, MJ Meagher, JA Gargac, GL Niebur, RK Roeder, DR Wagner Mineral deposition and vascular invasion of hydroxyapatite reinforced collagen scaffolds seeded with human adipose-derived stem cells Biomater Res, 2019-10-17;23(0):15. 2019-10-17 [PMID: 31641529] (Flourescense Microscopy, Human)	Flourescense Microscopy	Human
Alexandra Brahmer, Elmo Neuberger, Leona Esch-Heisser, Nils Haller, Malene Moeller Jorgensen, Rikke Baek et al. Platelets, endothelial cells and leukocytes contribute to the exercise-triggered release of extracellular vesicles into the circulation Journal of Extracellular Vesicles 2019-12-01 [PMID: 31191831]
JM Elsakr, JC Dunn, K Tennant, SK Zhao, K Kroeten, RC Pasek, DL Takahashi, TA Dean, DR Velez Edwa, CE McCurdy, KM Aagaard, AC Powers, JE Friedman, P Kievit, M Gannon Maternal Western-style diet affects offspring islet composition and function in a non-human primate model of maternal over-nutrition Mol Metab, 2019-04-12;0(0):. 2019-04-12 [PMID: 31036449] (IHC-P, Primate - Macaca fuscata (Japanese Macaque))	IHC-P	Primate - Macaca fuscata (Japanese Macaque)
Gliemann L, Rytter N, Piil P et al. The Endothelial Mechanotransduction Protein Platelet Endothelial Cell Adhesion Molecule-1 Is Influenced by Aging and Exercise Training in Human Skeletal Muscle. Front Physiol 12/18/2018 [PMID: 30618819]
Vinay Saunders, Jennifer M. Dewing, Tilman Sanchez-Elsner, David I. Wilson Expression and localisation of thymosin beta-4 in the developing human early fetal heart PLOS ONE 2018-11-09 [PMID: 30412598]
J Bourland, J Fradette, FA Auger Tissue-engineered 3D melanoma model with blood and lymphatic capillaries for drug development Sci Rep, 2018-09-04;8(1):13191. 2018-09-04 [PMID: 30181613] (IHC, Human)	IHC	Human
Angela Rey-Gallardo, Hannah Tomlins, Justin Joachim, Izajur Rahman, Phoebe Kitscha, Karen Frudd et al. Sequential binding of ezrin and moesin to L-selectin regulates monocyte protrusive behaviour during transendothelial migration Journal of Cell Science 2018-07-01 [PMID: 29777033]
M Holsti, A Wanhainen, C Lundin, M Björck, G Tegler, J Svensson, M Sund Circulating Vascular Basement Membrane Fragments are Associated with the Diameter of the Abdominal Aorta and Their Expression Pattern is Altered in AAA Tissue Eur J Vasc Endovasc Surg, 2018-04-12;0(0):. 2018-04-12 [PMID: 29656960] (IHC-Fr, Human)	IHC-Fr	Human
R Amer, L Tiosano, J Pe'er Leucine-Rich ?-2-Glycoprotein-1 (LRG-1) Expression in Retinoblastoma Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):685-692. 2018-02-01 [PMID: 29392314] (IHC, Human)	IHC	Human
L Gibot, T Galbraith, J Bourland, A Rogic, M Skobe, FA Auger Tissue-engineered 3D human lymphatic microvascular network for in vitro studies of lymphangiogenesis Nat Protoc, 2017-04-27;12(5):1077-1088. 2017-04-27 [PMID: 28448484] (IHC, Human)	IHC	Human
Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells Sci Rep, 2016-11-02;6(0):35680. 2016-11-02 [PMID: 27804979] (ICC, Human)	ICC	Human
Rachel S Kerr, Sharon Love, Eva Segelov, Elaine Johnstone, Beverly Falcon, Peter Hewett et al. Adjuvant capecitabine plus bevacizumab versus capecitabine alone in patients with colorectal cancer (QUASAR 2): an open-label, randomised phase 3 trial The Lancet Oncology 2016-11-01 [PMID: 27660192]
A Datta-Mann, JE Croy, L Schirtzing, S Torgerson, M Breyer, VJ Wroblewski Aberrant bispecific antibody pharmacokinetics linked to liver sinusoidal endothelium clearance mechanism in cynomolgus monkeys MAbs, 2016-07-01;8(5):969-82. 2016-07-01 [PMID: 27111637] (ICC, Primate)	ICC	Primate
Laure Gibot, Todd Galbraith, Bryan Kloos, Suvendu Das, Dan A. Lacroix, François A. Auger et al. Cell-based approach for 3D reconstruction of lymphatic capillaries in vitro reveals distinct functions of HGF and VEGF-C in lymphangiogenesis Biomaterials 2016-02-01 [PMID: 26694987]
Madeleine J. Oudin, Shannon K. Hughes, Nazanin Rohani, Mira N. Moufarrej, Joan G. Jones, John S. Condeelis et al. Characterization of the expression of the pro-metastatic Mena(INV) isoform during breast tumor progression Clinical & Experimental Metastasis 2016-03-01 [PMID: 26680363]
Mohamed Fouad Mansour, Mélissa Pelletier, Marie-Michèle Boulet, Dominique Mayrand, Gaétan Brochu, Stefane Lebel et al. Oxidative activity of 17 beta -hydroxysteroid dehydrogenase on testosterone in male abdominal adipose tissues and cellular localization of 17 beta -HSD type 2 Molecular and Cellular Endocrinology 2015-10-15 [PMID: 26123590]
Pang M, Georgoudaki A, Lambut L, Johansson J, Tabor V, Hagikura K, Jin Y, Jansson M, Alexander J, Nelson C, Jakobsson L, Betsholtz C, Sund M, Karlsson M, Fuxe J TGF-beta1-induced EMT promotes targeted migration of breast cancer cells through the lymphatic system by the activation of CCR7/CCL21-mediated chemotaxis. Oncogene, 2015-05-11;35(6):748-60. 2015-05-11 [PMID: 25961925] (IHC, Mouse)	IHC	Mouse
Tate C, Mc Entire J, Pallini R, Vakana E, Wyss L, Blosser W, Ricci-Vitiani L, D'Alessandris Q, Morgante L, Giannetti S, Larocca L, Todaro M, Benfante A, Colorito M, Stassi G, De Maria R, Rowlinson S, Stancato L A BMP7 Variant Inhibits Tumor Angiogenesis In Vitro and In Vivo through Direct Modulation of Endothelial Cell Biology. PLoS ONE, 2015-04-28;10(4):e0125697. 2015-04-28 [PMID: 25919028] (IHC-Fr, Human)	IHC-Fr	Human
Crosley , Erin J, Dunk , Caroline, Beristain , Alexande, Christians , Julian K IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells. Reprod Biol Endocrinol, 2014-12-04;12(0):123. 2014-12-04 [PMID: 25475528] (IHC-P, Human)	IHC-P	Human
Kim Aubin, Caroline Vincent, Maryse Proulx, Dominique Mayrand, Julie Fradette Creating capillary networks within human engineered tissues: Impact of adipocytes and their secretory products Acta Biomaterialia 2015-01-01 [PMID: 25278444] (Immunohistochemistry, Human)	Immunohistochemistry	Human
Pound L, Comstock S, Grove K Consumption of a Western-style diet during pregnancy impairs offspring islet vascularization in a Japanese macaque model. Am J Physiol Endocrinol Metab, 2014-05-20;307(1):E115-23. 2014-05-20 [PMID: 24844258] (IHC-P, Primate - Macaca mulatta (Rhesus Macaque))	IHC-P	Primate - Macaca mulatta (Rhesus Macaque)
Blosser , Wayne, Vakana , Eliza, Wyss , Lisa V, Swearingen , Michelle, Stewart , Julie, Stancato , Louis, Tate , Courtney A method to assess target gene involvement in angiogenesis in vitro and in vivo using lentiviral vectors expressing shRNA. PLoS ONE, 2014-04-23;9(4):e96036. 2014-04-23 [PMID: 24759702] (ICC, Human)	ICC	Human
Tang , W, Yu , F, Yao , H, Cui , X, Jiao , Y, Lin , L, Chen , J, Yin , D, Song , E, Liu , Q miR-27a regulates endothelial differentiation of breast cancer stem like cells. Oncogene, 2013-06-10;33(20):2629-38. 2013-06-10 [PMID: 23752185] (ICC, IHC-Fr, Western Blot, Human)	ICC, IHC-Fr, Western Blot	Human
Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop. BMC Cancer, 2013-03-26;13(0):154. 2013-03-26 [PMID: 23530721] (ICC, IHC-Fr, Human)	ICC, IHC-Fr	Human
Fan , L, Lindsley , S R, Comstock , S M, Takahashi , D L, Evans , A E, He , G-W, Thornburg , K L, Grove , K L Maternal high-fat diet impacts endothelial function in nonhuman primate offspring. Int J Obes (Lond), 2012-03-27;37(2):254-62. 2012-03-27 [PMID: 22450853] (IHC-P, Primate - Macaca fuscata (Japanese Macaque))	IHC-P	Primate - Macaca fuscata (Japanese Macaque)
Prokopi M, Pula G, Mayr U, Devue C, Gallagher J, Xiao Q, Boulanger CM, Westwood N, Urbich C, Willeit J, Steiner M, Breuss J, Xu Q, Kiechl S, Mayr M Proteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures. Blood, 2009-04-15;114(3):723-32. 2009-04-15 [PMID: 19369228] (ICC, Human)	ICC	Human
Kelly MA, Hirschi KK Signaling hierarchy regulating human endothelial cell development. Arterioscler. Thromb. Vasc. Biol., 2009-02-12;29(5):718-24. 2009-02-12 [PMID: 19213939] (ICC, Human)	ICC	Human
Shen HC, He M, Powell A, Adem A, Lorang D, Heller C, Grover AC, Ylaya K, Hewitt SM, Marx SJ, Spiegel AM, Libutti SK Recapitulation of pancreatic neuroendocrine tumors in human multiple endocrine neoplasia type I syndrome via Pdx1-directed inactivation of Men1. Cancer Res., 2009-02-10;69(5):1858-66. 2009-02-10 [PMID: 19208834] (IHC-Fr, Mouse)	IHC-Fr	Mouse
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Review for CD31/PECAM-1 Antibody (AF806) (1) 51

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(Based on 1 review)

We have 1 review tested in 1 species: Human.

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reviewed by:
Ivan Osinnii

IHC-Fr

Human

08/21/2023

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Application	Immunohistochemistry-Frozen
Sample Tested	colon,Human Colon
Species	Human
Lot	DGX0523031

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Immunofluorescence on freshly-frozen PFA-fixed cryosections

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The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells. Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol... Read full blog post.

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Effects of SDF-1-CXCR4/CXCR7 pathway on MSC chemotaxis in vitro.(A) The chemotaxis in response to SDF-1 alpha (10 ng/ml for 12 h) was performed in the NP-MSCs and HP-MSCs treated with a neutralizing anti-CXCR4 antibody, an anti-CXCR7 antibody, and the respective isotype-matched control antibodies. *P<0.05, vs NP-MSCs; †P<0.05, vs the respective isotype-matched control antibodies. (B) NP-MSCs were transiently overexpressed with CXCR4 using pORF9-mCXCR4 vector or with CXCR7 using pORF9-mCXCR7 vector (n = 6). A negative control empty (pORF9-MCS) vector was used. (C) The transfected cells were subjected to chemotaxis in response to the indicated concentrations of SDF-1 alpha for 12 h. *P<0.05, vs the empty vector. (D and E) Western blot analysis (D) and FCM (E) were performed to determine the intracellular and extracellular expression of both CXCR4 and CXCR7 in the cells treated with or without SDF-1 alpha (50 ng/ml for 60 min). (F) The chemotaxis in response to SDF-1 alpha was performed in the cells treated with or without SDF-1 alpha . Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22511954), licensed under a CC-BY license. Not internally tested by R&D Systems.

Sheep IgG Isotype Control [Unconjugated]
5-001-A

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.

TNF-alpha [Unconjugated]
210-TA-005

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Immunohistochemistry-Frozen CD31/PECAM-1 AF806

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	Ivan Osinnii 08/21/2023
Application:	IHC-Fr
Species:	Human

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PECAM1

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

CD31/PECAM-1 Antibody [Unconjugated]

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Publications for CD31/PECAM-1 Antibody (AF806)(71)

Review for CD31/PECAM-1 Antibody (AF806) (1) 51

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