CD31/PECAM-1 Antibody (MEC13.3) - BSA Free

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 9 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-P, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, In Vivo, In-vitro.

Showing Publications 1 - 10 of 29. Show All 29 Publications. Collapse Publications.

Publications using NB600-1475	Applications	Species
Yang C, Gao Q, Xu N et al. Human Dental Pulp Stem Cells Are Subjected to Metabolic Reprogramming and Repressed Proliferation and Migration by the Sympathetic Nervous System via ?1B-Adrenergic Receptor Journal of endodontics 2023-09-27 [PMID: 37769871]
Ugur M, Labios RJ, Fenton C et al. Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networks Immunity 2023-07-07 [PMID: 37463581] (ICC/IF) Details: Alexa Fluor 700 conjugation used	ICC/IF
Pang L, Dunterman M, Xuan W et al. Circadian regulator CLOCK promotes tumor angiogenesis in glioblastoma Cell reports 2023-02-14 [PMID: 36795563] (IHC, Human)	IHC	Human
Deng W, Guo S, van Veluw SJ et al. Effects of cerebral amyloid angiopathy on the brain vasculome Aging cell 2022-07-18 [PMID: 35851991] (IF/IHC, Mouse)	IF/IHC	Mouse
Zhang Y, Wang D, Zhao Z et al. Nephronectin promotes cardiac repair post myocardial infarction via activating EGFR/JAK2/STAT3 pathway International Journal of Medical Sciences 2022-05-13 [PMID: 35693734] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence)	Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence
Chen y, Wang H, yang Q et al. Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis Theranostics 2022-01-01 [PMID: 35154475] (IHC-P, Human)	IHC-P	Human
Yang C, Lavayen BP, Liu L Et al. Neurovascular protection by adropin in experimental ischemic stroke through an endothelial nitric oxide synthase-dependent mechanism Redox biology 2021-11-22 [PMID: 34826783] (IF/IHC, Mouse)	IF/IHC	Mouse
Park M, Kim J, Kim T Et al. REDD1 is a determinant of low-dose metronomic doxorubicin-elicited endothelial cell dysfunction through downregulation of VEGFR-2/3 expression Experimental & molecular medicine 2021-10-25 [PMID: 34697389] (IF/IHC, Mouse)	IF/IHC	Mouse
Mao XW, Nishiyama NC, Byrum SD et al. Spaceflight induces oxidative damage to blood-brain barrier integrity in a mouse model FASEB J. 2020-09-26 [PMID: 32981077] (IF/IHC, Mouse)	IF/IHC	Mouse
Li A The Influence of Ontogeny and Physiological Microbial Exposure on Macrophage Maintenance and Function Thesis (ICC/IF, Mouse)	ICC/IF	Mouse
Wei W, Liu Q, Jiang D et al. Tissue Factor-Targeted ImmunoPET Imaging and Radioimmunotherapy of Anaplastic Thyroid Cancer Adv Sci (Weinh) 2020-07-17 [PMID: 32670751]
Honig MG, Dorian CC, Worthen JD et al. Progressive long-term spatial memory loss following repeat concussive and subconcussive brain injury in mice, associated with dorsal hippocampal neuron loss, microglial phenotype shift, and vascular abnormalities European Journal of Neuroscience 2021-09-01 [PMID: 32090401]
Li W, Zhan M, Quan YY et al. Modulating the tumor immune microenvironment with sunitinib malate supports the rationale for combined treatment with immunotherapy Int. Immunopharmacol. 2020-02-17 [PMID: 32078941] (ICC/IF, Mouse)	ICC/IF	Mouse
Liu F, Liu Q, Yuan F et al. Erg mediates downregulation of claudin-5 in the brain endothelium of a murine experimental model of cerebral malaria FEBS Lett. 2019-07-04 [PMID: 31271645]
Niu F, Liao K, Hu G et al. Cocaine-induced release of CXCL10 from pericytes regulates monocyte transmigration into the CNS. J. Cell Biol. 2019-01-09 [PMID: 30626719] (ICC/IF, Human)	ICC/IF	Human
Abraham V, Cao G, Parambath A et al. Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination Int J Onc [PMID: 29845213] (Mouse)		Mouse
Perales-Puchalt A, Perez-Sanz J, Payne KK et al. Frontline Science: Microbiota reconstitution restores intestinal integrity after cisplatin therapy. J. Leukoc. Biol. 2018-03-14 [PMID: 29537705] (IF/IHC)	IF/IHC
Hernandez R, England CG, Yang Y et al. ImmunoPET imaging of tissue factor expression in pancreatic cancer with (89)Zr-Df-ALT-836. J Control Release 2017-08-24 [PMID: 28843831] (Mouse)		Mouse
DeMars KM, Pacheco SC, Yang C et al. Selective Inhibition of Janus Kinase 3 Has No Impact on Infarct Size or Neurobehavioral Outcomes in Permanent Ischemic Stroke in Mice. Front Neurol 2017-08-09 [PMID: 28790974] (ICC/IF, Mouse)	ICC/IF	Mouse
Takeda A, Hossain MS, Rantakari P et al. Thymocytes in Lyve1-CRE/S1pr1(f/f) Mice Accumulate in the Thymus due to Cell-Intrinsic Loss of Sphingosine-1-Phosphate Receptor Expression. Front Immunol. 2016-11-23 [PMID: 27877175] (IF/IHC, Mouse)	IF/IHC	Mouse
Ensan, S, Li, A Et al. Self-renewing resident arterial macrophages arise from embryonic CX3CR1(+) precursors and circulating monocytes immediately after birth. Nat Immunol 2016-02-01 [PMID: 26642357] (FLOW, Human)	FLOW	Human
Salatino M, Croci DO, Laderach DJ et al. Regulation of galectins by hypoxia and their relevance in angiogenesis: strategies and methods. Methods Mol. Biol. 2014-09-25 [PMID: 25253148]
Takeuchi K, Yanai R, Kumase F et al. EGF-Like-Domain-7 Is Required for VEGF-Induced Akt/ERK Activation and Vascular Tube Formation in an Ex Vivo Angiogenesis Assay. PLoS ONE 2014-03-20 [PMID: 24647208] (In-vitro, ICC/IF, Mouse)	In-vitro, ICC/IF	Mouse
Voswinckel R, Ziegelhoeffer T, Heil M et al. Circulating vascular progenitor cells do not contribute to compensatory lung growth. Circ Res;93(4):372-9. 2003-08-22 [PMID: 12881479] (IHC-P, Mouse)	IHC-P	Mouse
DeLisser HM, Christofidou-Solomidou M, Strieter RM, Burdick MD, Robinson CS, Wexler RS, Kerr JS, Garlanda C, Merwin JR, Madri JA, Albelda SM. Involvement of endothelial PECAM-1/CD31 in angiogenesis. Am J Pathol;151(3):671-7. 1997-09-01 [PMID: 9284815] (In Vivo, Mouse)	In Vivo	Mouse
Christofidou-Solomidou M, Nakada MT, Williams J et al. Neutrophil platelet endothelial cell adhesion molecule-1 participates in neutrophil recruitment at inflammatory sites and is down-regulated after leukocyte extravasation. J Immunol;158(10):4872-8. 1997-05-15 [PMID: 9144503]
Vecchi A, Garlanda C, Lampugnani MG, Resnati M, Matteucci C, Stoppacciaro A, Schnurch H, Risau W, Ruco L, Mantovani A et al. Monoclonal antibodies specific for endothelial cells of mouse blood vessels. Eur J Cell Biol;63(2):247-54. 1994-04-01 [PMID: 8082649]
Rosenblum WI, Murata S, Nelson GH, Werner PK, Ranken R, Harmon RC. Anti-CD31 delays platelet adhesion/aggregation at sites of endothelial injury in mouse cerebral arterioles. Am J Pathol;145(1):33-6. 1994-07-01 [PMID: 8030753] (In Vivo, Mouse)	In Vivo	Mouse
Baldwin HS, Shen HM, Yan HC, DeLisser HM, Chung A, Mickanin C, Trask T, Kirschbaum NE, Newman PJ, Albelda SM et al. Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31): alternatively spliced, functionally distinct isoforms expressed during mammalian cardiovascular development. Development;120(9):2539-53. 1994-09-01 [PMID: 7956830] (FLOW, Mouse)	FLOW	Mouse
Show All 29 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	IHC-Fr	Human	08/29/2017	View Summary Application Immunohistochemistry-Frozen Sample Tested Non-small cell lung cancer Species Human Lot D-2
Enlarge	5 reviewed by: Christopher England	IHC-Fr	Mouse	02/24/2017	View Summary Application Immunohistochemistry-Frozen Sample Tested 4T1 tissue Species Mouse Comments Comments Green is the CD31, which looked perfect!

Array NB600-1475

• CD31/PECAM-1 Antibodies
• CD31/PECAM-1 Antibody Pair
• CD31/PECAM-1 ELISA Kits
• CD31/PECAM-1 Lysate
• CD31/PECAM-1 Protein
• CD31/PECAM-1 Proteins and Enzymes
• CD31/PECAM-1 ELISAs