Why are there different molecular weight bands in western blot images for two of your antibodies (NBP1-84432 and H00010871-B01P) and overexpression lysate NBL1-08931?

Name: CD300c Antibody Pair [HRP]
Brand: Novus Biologicals
SKU: NBP2-79371
Price: 1009 USD

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A variety of band patterns can be expected when detecting CD300C. This protein has multiple glycosliation post-translational modifications that will change its molecular weight, and which vary in different experimental conditions. If you look at the uniprot link listed below, you can view more information on the specific PTMs for CD300C:https://www.uniprot.org/uniprot/Q08708#sequencesWe can be assured that these varying band patterns are indeed detection of CD300C by the absence of bands in the negative control. Any non-specific binding would show in both the negative and positive control, which is not the case here.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	ELISA
Conjugate	HRP
Standard Curve Range	9.38-600 pg/ml (example only; lot dependent)
Sensitivity	9.4 pg/ml (example only; lot dependent)

Catalog# & Conjugate	Size	Price
HRP NBP2-79371-5Plates

Conjugate	Catalog #	Availability	Size	Price
HRP	NBP2-79371-5Plates

Description	Solid Phase sandwich ELISA for the quantitative determination of Human CD300c/LMIR2.
Additional Information	Recombinant Monoclonal Antibody
Specificity	This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CD300c/LMIR2.
Standard Curve Range	9.38-600 pg/ml (example only; lot dependent)
Sensitivity	9.4 pg/ml (example only; lot dependent)
Assay Type	Sandwich ELISA
Sample Volume	100 ul
Gene	CD300C

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