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CD24 Antibody (ML5) - Azide and BSA Free

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Immunocytochemistry/Immunofluorescence: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636] - MCF-7 breast cancer cells. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636] - MCF-7 breast cancer cells. Image from the standard format of this antibody.
Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636] - Analysis using the FITC conjugate of NB100-77903. Staining of Human peripheral blood lymphocytes stained ML5 FITC.
Flow Cytometry: CD24 Antibody (ML5) - Azide and BSA Free [NBP2-80636] - Whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC Image from the standard format of this antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
ML5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-77903AF350
Alexa Fluor 405
NB100-77903AF405
Alexa Fluor 488
NB100-77903AF488
Alexa Fluor 532
NB100-77903AF532
Alexa Fluor 594
NB100-77903AF594
Alexa Fluor 647
NB100-77903AF647
Alexa Fluor 700
NB100-77903AF700
Alexa Fluor 750
NB100-77903AF750
Allophycocyanin
NB100-77903APC
Biotin
NB100-77903B
CoraFluor 1
NB100-77903CL1
DyLight 350
NB100-77903UV
DyLight 405
NB100-77903V
DyLight 488
NB100-77903G-0.1ml
DyLight 550
NB100-77903R-0.1ml
DyLight 594
NB100-77903DL594
DyLight 650
NB100-77903C-0.1ml
DyLight 680
NB100-77903FR
DyLight 755
NB100-77903IR
FITC
NB100-77903F
HRP
NB100-77903H
Janelia Fluor 525
NB100-77903JF525
Janelia Fluor 585
NB100-77903JF585
Janelia Fluor 635
NB100-77903JF635
Janelia Fluor 669
NB100-77903JF669
PE
NB100-77903PE
PerCP
NB100-77903PCP
Unconjugated
NB100-77903
mFluor Violet 450 SE
NB100-77903MFV450
mFluor Violet 500 SE
NB100-77903MFV500
mFluor Violet 610 SE
NB100-77903MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-77903
BSA Free
NB100-77903
BSA Free
NB100-77903SS
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CD24 Antibody (ML5) - Azide and BSA Free Summary

Immunogen
The immunogen was the full length version of the protein: CD24 (ML5)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD24
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 0.125 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 5-25 ug/ml
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Expected Cross-Reactivity based on immunogen sequence: Chimpanzee, Baboon

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CD24 Antibody (ML5) - Azide and BSA Free

  • CD 24
  • CD24 antigen (small cell lung carcinoma cluster 4 antigen)
  • CD24 antigen
  • CD24 molecule
  • CD24
  • CD24A
  • CD24Asignal transducer CD24
  • FLJ22950
  • FLJ43543
  • MGC75043
  • Small cell lung carcinoma cluster 4 antigen

Background

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CD24