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CD24 Antibody (4F4E10) - BSA Free

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Western Blot: CD24 Antibody (4F4E10) [NBP2-37352] - Analysis using CD24 mAb against HEK293 (1) and CD24 (AA: 15-80)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: CD24 Antibody (4F4E10) [NBP2-37352] - Analysis of Hela cells using CD24 mouse mAb (green) and negative control (red).
Western Blot: CD24 Antibody (4F4E10) [NBP2-37352] - Analysis using CD24 mAb against human CD24 recombinant protein. (Expected MW is 32.1 kDa)
ELISA: CD24 Antibody (4F4E10) [NBP2-37352] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, CyTOF-ready
Clone
4F4E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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Catalog# & Formulation Size Price
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CD24 Antibody (4F4E10) - BSA Free Summary

Immunogen
Purified recombinant fragment of human CD24 (AA: 15-80) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD24
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for CD24 Antibody (4F4E10) - BSA Free

  • CD 24
  • CD24 antigen (small cell lung carcinoma cluster 4 antigen)
  • CD24 antigen
  • CD24 molecule
  • CD24
  • CD24A
  • CD24Asignal transducer CD24
  • FLJ22950
  • FLJ43543
  • MGC75043
  • Small cell lung carcinoma cluster 4 antigen

Background

CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD24