CD161 Antibody (10/78) - BSA Free

Images

 
Flow Cytometry: CD161 Antibody (10/78) [NB100-65297] - Rat splenocytes were stained with Anti-Rat CD3 APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat CD161 Monoclonal Antibody (Catalog# NB100-65297) or ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, IHC, IP
Clone
10/78
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CD161 Antibody (10/78) - BSA Free Summary

Immunogen
Purified splenic NK cells from the LEW rat strain.
Specificity
Recognizes the rat CD161 protein (also known as NKR-PI) a 60kD glycoprotein expressed on rat NK cells and T cell subpopulations. This competes with clone 3.2.3 for binding to antigen.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KLRB1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Reviewed Applications
Read 1 Review rated 1
using
NB100-65297 in the following applications:

Publications
Read Publication using
NB100-65297 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD161 Antibody (10/78) - BSA Free

  • CD161 antigen
  • CD161
  • CLEC5B
  • CLEC5BC-type lectin domain family 5 member B
  • HNKR-P1a
  • killer cell lectin-like receptor subfamily B member 1
  • killer cell lectin-like receptor subfamily B, member 1
  • KLRB1
  • Ly59
  • Natural killer cell surface protein P1A
  • NKR
  • NKRP1
  • NKR-P1
  • NKRP1A
  • NKR-P1AMGC138614
  • NKRP1ANKR

Background

CD161, also known as KLRB1, NK1.1, and NKR-P1A is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-gamma secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161+ cell populations are depleted in ulcerative colitis, Grave's disease, and AIDS, although CD161+ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR-P1B and D, and the stimulatory NKR-P1A, C, and F.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD161 Antibody (NB100-65297)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: FLOW.


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Review for CD161 Antibody (NB100-65297) (1) 11

Average Rating: 1
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-65297:
Filter by Applications
IHC-P
(1)
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Filter by Species
Rat
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD161 NB100-65297
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1
reviewed by:
Vincent Eckert
IHC-P Rat 11/12/2019
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedRat liver,Rat Lung Tissue
SpeciesRat
LotCLCP02

Comments

Comments***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Product General Protocols

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FAQs for CD161 Antibody (NB100-65297). (Showing 1 - 1 of 1 FAQ).

  1. I see that this antibody is listed for use in IHC...would that include IHC on formalin-fixed-paraffin-embedded tissue?
    • NB100-65297 has been tested and validated for use with frozen tissues however its performance has not been evaluated in paraffin embedded tissues yet and therefore cannot be guaranteed. If you would be interested in testing this antibody in IHC-Paraffin please take a look at our Innovators Reward Program.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Vincent Eckert
11/12/2019
Application: IHC-P
Species: Rat

Bioinformatics

Gene Symbol KLRB1
Entrez
Uniprot