![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Immunocytochemistry/Immunofluorescence (ICC/IF) analysis of RAW264.7 cells using CD11b antibody (M1/70.15) [NB600-1327] with Dylight 488 conjugated secondary (green). Nuclei and tubulin were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and Dylight 550 (red). Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80626-img0004.jpg "Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Immunocytochemistry/Immunofluorescence (ICC/IF) analysis of RAW264.7 cells using CD11b antibody (M1/70.15) [NB600-1327] with Dylight 488 conjugated secondary (green). Nuclei and tubulin were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and Dylight 550 (red). Image from the standard format of this antibody.")
| Reactivity | Hu, Mu, RbSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, IP, CyTOF-ready |
| Clone | M1/70.15 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Rat Monoclonal CD11b Antibody (M1/70.15) was made to T cell enriched splenocytes from B10 mice. |
| Isotype | IgG2b Lambda |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | ITGAM |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. NB600-1327 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. This antibody is CyTOF ready. |
| Theoretical MW | 127.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
![Flow Cytometry L-Selectin/CD62L Antibody (4G8) [Unconjugated]](https://images.novusbio.com/images/antibody/bba24_human-l-selectin-cd62l-mab-cl-4g8-flow-cytometry-822024174319..jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for CD11b Antibody (NBP2-80626)Find related products by research area.
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Antibody treatment can generate microglia-like cells from bone marrow By Jennifer Sokolowski, MD, PhD.Microglia play important roles in the brain in both homeostatic and pathological conditions, acting to clear debris and dying cells. There is evidence to suggest that microglial dys... Read full blog post. |
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TMEM 119 is a specific marker of microglia cells By Jennifer Sokolowski, MD, PhD.Microglia are a major immune-cell component in the brain. They ingest and degrade dead cells, debris, and foreign material and interact with other immune cells to orchestrate centra... Read full blog post. |
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Topics in CD11b: The innate immune response Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens. Integrin... Read full blog post. |
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CD11b - More than a microglial marker The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the... Read full blog post. |
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CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a... Read full blog post. |
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Adhesion Receptor Molecule CD11b/c is the Point of Entry for many Infectious Diseases Pathogenic microorganisms utilize a variety of cell surface receptors to gain entry into host cells and to bypass the natural defense mechanisms. One of the most prominent receptors used in this fashion is the leukocyte adhesion receptor CD11b/c. This... Read full blog post. |
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CD11b/CD18 and Neutrophil-Epithelial Interactions as Targets for Anti-Inflammatory Therapies The beta-2 integrins, a family of four cell surface transmembrane glycoproteins expressed only on leukocytes, include CD11a/CD18, CD11b/CD18, CD11c/CD18, and CD11d/CD18. They consist of a common beta subunit (CD18) and homologous alpha subunits (CD11a... Read full blog post. |
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The CD11b Antibody: A Marker for Microglial Cells Microglia are the resident macrophages of the central nervous system, and the first line of immune defense. Pioneering antibody research in the 1990's identified the Integrin beta 2 protein (also called ITGB2, complement receptor 3, CR3, CD18, and Mac... Read full blog post. |
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CD11b Expression, Leukocyte Adhesion and the Innate Immune System What is CD11b?CD11b is an integrin family member which pairs with CD18 to form the CR3 heterodimer. CD11b is expressed on the surface of many leukocytes including monocytes, neutrophils, natural killer cells, granulocytes and macrophages, as well as o... Read full blog post. |
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| Gene Symbol | ITGAM |
![Immunohistochemistry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80626-img0005.jpg "Immunohistochemistry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Immunohistochemical analysis of frozen lymph node section using CD11b Antibody (M1/70.15) [NB600-1327] followed by staining with HRP-conjugated secondary antibody. Image from the standard format of this antibody.")
![Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80626-img0008.jpg "Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Characterization of microglia cultures by multiple staining with CD11b Antibody (M1/70.15) [NB600-1327] (panels a. & c.) and anti GFAP (panels b & d). Nuclei appear blue due to 4',6-diamidino-2-phenylindole (DAPI) counter staining. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80626-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Characterization of microglia cultures by multiple staining with CD11b Antibody (M1/70.15) [NB600-1327] (panels a. & c.) and anti GFAP (panels b & d). Nuclei appear blue due to 4',6-diamidino-2-phenylindole (DAPI) counter staining. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Immunocytochemical/Immunofluorescent analysis in mouse primary microglia. Cells were treated with lysophosphatidic acid (LPA) or LPA and an LPAR5 inhibitor in order to investigate the expression of iNOS after 24 hours. CD11b Antibody (M1/70.15) [NB600-1327] was used in a dilution 1:100 in order to stain primary murine microglia. Cy2 (green) and Cy3 (red) were used as secondary antibodies. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80626-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Immunocytochemical/Immunofluorescent analysis in mouse primary microglia. Cells were treated with lysophosphatidic acid (LPA) or LPA and an LPAR5 inhibitor in order to investigate the expression of iNOS after 24 hours. CD11b Antibody (M1/70.15) [NB600-1327] was used in a dilution 1:100 in order to stain primary murine microglia. Cy2 (green) and Cy3 (red) were used as secondary antibodies. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Immunocytochemical/Immunofluorescent staining in untreated and treated mouse microglia cells using CD11b Antibody (M1/70.15) [NB600-1327]. Image from a verified customer review. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80626-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Immunocytochemical/Immunofluorescent staining in untreated and treated mouse microglia cells using CD11b Antibody (M1/70.15) [NB600-1327]. Image from a verified customer review. Image from the standard format of this antibody.")
![Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - A surface stain was performed on Raw264.7 cells with CD11b (M1/70.15) Antibody [NB600-1327R] (blue) and a matched isotype control (orange), both conjugated to DyLight 550. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80626-img0006.jpg "Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - A surface stain was performed on Raw264.7 cells with CD11b (M1/70.15) Antibody [NB600-1327R] (blue) and a matched isotype control (orange), both conjugated to DyLight 550. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Image from the standard format of this antibody.")
![Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Staining of mouse peritoneal macrophages using the Alexa Fluor (R) 488 conjugate of CD11b Antibody (M1/70.15) [NB600-1327AF488].](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80626-img0007.jpg "Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Staining of mouse peritoneal macrophages using the Alexa Fluor (R) 488 conjugate of CD11b Antibody (M1/70.15) [NB600-1327AF488].")
![Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]: RPE - Alexa Fluor 750 Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/CD11b-Antibody-M1-70-15-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80626-img0009.jpg "Flow Cytometry: CD11b Antibody (M1/70.15) - Azide and BSA Free [NBP2-80626] - Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b Antibody (M1/70.15) [NB600-1327]: RPE - Alexa Fluor 750 Image from the standard format of this antibody.")
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![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![N/A CD14 [HRP]](https://images.novusbio.com/images2/CD14_DC140_ELISA_42.jpg)
![N/A CD14 [HRP]](https://images.novusbio.com/images2/DATA_CD14_DC140_ELISA_597.jpg)
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