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Human CCL8/MCP-2 ELISA Kit (Colorimetric)

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ELISA: Human CCL8/MCP-2 ELISA Kit (Colorimetric) [NBP2-68059] - Samples were spiked with high concentrations of Human CCL8/MCP-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human CCL8/MCP-2 ELISA Kit (Colorimetric) [NBP2-68059] - Samples were spiked with high concentrations of Human CCL8/MCP-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Human CCL8/MCP-2 ELISA Kit (Colorimetric) [NBP2-68059] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
6.25 - 400 pg/mL
Sensitivity
3.75 pg/mL

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Human CCL8/MCP-2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human MCP-2/CCL8 in samples. No significant cross-reactivity or interference between Human MCP-2/CCL8 and analogues was observed.
Standard Curve Range
6.25 - 400 pg/mL
Sensitivity
3.75 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.57%
Intra-Assay
CV% < 5.36%
Spike Recovery
84-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CCL8

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human CCL8/MCP-2 ELISA Kit (Colorimetric)

  • C-C motif chemokine 8
  • CCL8
  • chemokine (C-C motif) ligand 8
  • HC14
  • MCP2
  • MCP-2
  • member 8 (monocyte chemotactic protein 2)
  • monocyte chemoattractant protein 2
  • SCYA10
  • small-inducible cytokine A8

Background

MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human CCL8/MCP-2
ELISA Kit (Colorimetr ...
NBP2-68059
Human CCL8/MCP-2 ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

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Product PDFs

  SDS
  Human CCL8/MCP-2 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol CCL8