Reactivity | HuSpecies Glossary |
Applications | WB, B/N |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant human CCL8/MCP-2 Gln24-Pro99 Accession # P80075 |
Specificity | Detects human CCL8/MCP‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 40% cross‑reactivity with recombinant human (rh) HCC-1 is observed, less than 10% cross‑reactivity with rhEotaxin is observed, and less than 5% cross‑reactivity with rhMCP-1 is observed. Neutralizes the biological activity of rhCCL8. This antibody will not neutralize the biological activity of rhMCP-1, recombinant mouse JE, or rhMCP-3. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | CCL8 |
Purity Statement | Antigen Affinity-purified |
Endotoxin Note | <0.10 EU per 1 μg of the antibody by the LAL method. |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2.
Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Publication using AF-281-NA | Applications | Species |
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Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D Expression and regulation of chemokines in murine and human type 1 diabetes. Diabetes, 2011-12-30;61(2):436-46. 2011-12-30 [PMID: 22210319] (Flow Cytometry, Human) | Flow Cytometry | Human |
Secondary Antibodies |
Isotype Controls |
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