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CCL24/Eotaxin-2/MPIF-2 Antibody

 (7 Publications)
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Recombinant Mouse CCL24/Eotaxin‑2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL24/Eotaxin‑2 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL24/Eotaxin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF528). The ND50 is typically 0.5-2.5 µg/mL.
Recombinant Mouse CCL24/Eotaxin‑2 (Catalog # 1017-MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF528
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Catalog# & Formulation Size Price
Carrier Free
AF528-SP
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CCL24/Eotaxin-2/MPIF-2 Antibody Summary

Immunogen
E. coli-derived recombinant mouse CCL24/Eotaxin‑2/MPIF‑2
Ile24-Val119
Accession # Q9JKC0
Specificity
Detects CCL24/Eotaxin‑2/MPIF‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Eotaxin-2 is observed, and less than 1% cross-reactivity with recombinant mouse Eotaxin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CCL24
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF528 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL24/Eotaxin-2/MPIF-2 Antibody

  • CCL24
  • chemokine (C-C motif) ligand 24
  • Ckb-6
  • Eosinophil chemotactic protein 2
  • Eotaxin-2
  • member 24
  • MPIF2
  • MPIF-2
  • MPIF2Myeloid progenitor inhibitory factor 2
  • MPIF-2Small-inducible cytokine A24
  • SCYA24CK-beta-6

Background

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCL24/Eotaxin-2/MPIF-2 Antibody (AF528)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Flow Cytometry, IHC-P, Neutralization.


Filter By Application
Flow Cytometry
(1)
IHC-P
(1)
Neutralization
(2)
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Mouse
(4)
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Showing Publications 1 - 7 of 7.
Publications using AF528 Applications Species
Cho-Hao Lin, Fatemeh Talebian, Yang Yang, Jianmin Zhu, Jin-Qing Liu, Bolin Zhao et al. CD200R signaling contributes to unfavorable tumor microenvironment through regulating production of chemokines by tumor-associated myeloid cells iScience 6/16/2023 [PMID: 37275530]
Sonia Ventura, Florencia Cano, Yashaswini Kannan, Felix Breyer, Michael J. Pattison, Mark S. Wilson et al. A20-binding inhibitor of NF-kappa B (ABIN) 2 negatively regulates allergic airway inflammation Journal of Experimental Medicine 11/5/2018 [PMID: 30337469]
Y Kannan, LJ Entwistle, VS Pelly, J Perez-Llor, AW Walker, SC Ley, MS Wilson TPL-2 restricts Ccl24-dependent immunity to Heligmosomoides polygyrus PLoS Pathog., 2017-07-31;13(7):e1006536. 2017-07-31 [PMID: 28759611] (Neutralization, Mouse) Neutralization Mouse
Macrophages and cardiac fibroblasts are the main producers of eotaxins and regulate eosinophil trafficking to the heart Eur J Immunol, 2016-10-25;0(0):. 2016-10-25 [PMID: 27621211] (IHC-P, Mouse) IHC-P Mouse
Oh Sasaki, Mitsuru Imamura, Yusuke Yamazumi, Hiroaki Harada, Taku Matsumoto, Katsuhide Okunishi et al. Alendronate Attenuates Eosinophilic Airway Inflammation Associated with Suppression of Th2 Cytokines, Th17 Cytokines, and Eotaxin-2 The Journal of Immunology 9/15/2013 [PMID: 23935198]
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D Comprehensive assessment of chemokine expression profiles by flow cytometry. J. Clin. Invest., 2010-02-08;120(3):907-23. 2010-02-08 [PMID: 20197626] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Takeda A, Baffi JZ, Kleinman ME, Cho WG, Nozaki M, Yamada K, Kaneko H, Albuquerque RJ, Dridi S, Saito K, Raisler BJ, Budd SJ, Geisen P, Munitz A, Ambati BK, Green MG, Ishibashi T, Wright JD, Humbles AA, Gerard CJ, Ogura Y, Pan Y, Smith JR, Grisanti S, Hartnett ME, Rothenberg ME, Ambati J CCR3 is a target for age-related macular degeneration diagnosis and therapy. Nature, 2009-06-14;460(7252):225-30. 2009-06-14 [PMID: 19525930] (Neutralization, Mouse) Neutralization Mouse

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Bioinformatics

Gene Symbol CCL24
Uniprot