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CCL15/MIP-1 delta Antibody (52130) [Unconjugated]

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Recombinant Human CCL15/MIP‑1δ (Catalog # 363-MG) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP‑1δ (16 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL15/MIP‑1δ Mono­clonal Antibody (Catalog # MAB363). The ND50 is typically 3-15 µg/mL.
Recombinant Human CCL15/MIP‑1δ (Catalog # 363-MG) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap)
Clone
52130
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB363-SP
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CCL15/MIP-1 delta Antibody (52130) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CCL15/MIP-1δ
Gln22-Ile113
Accession # Q16663.2
Specificity
Detects human CCL15/MIP‑1δ in ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CCL15
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 3-15 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human CCL15/MIP-1delta Biotinylated Antibody (Catalog number BAF363)
Standard: Recombinant Human CCL15/MIP-1delta 92 aa (Catalog number 363-MG)
Publications
Read Publications using
MAB363 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL15/MIP-1 delta Antibody (52130) [Unconjugated]

  • CCL15
  • chemokine (C-C motif) ligand 15
  • Chemokine CC-2
  • HCC-2
  • HCC-2Lkn-1
  • Leukotactin 1
  • Leukotactin-1
  • LKN1
  • LKN-1
  • Macrophage inflammatory protein 5
  • MIP1 delta
  • MIP-1 delta
  • MIP5
  • MIP-5
  • MIP-5MIP-1d
  • Mrp-2b
  • NCC-3
  • NCC-3MIP-1D
  • NCC3MRP-2B
  • new CC chemokine 3
  • SCYA15member 15
  • Small-inducible cytokine A15

Background

CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma , respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1δ is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

  1. Wang, W. et al. (1998) J. Clinical Immunol. 18:214

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CCL15/MIP-1 delta
Antibody (52130) [Unc ...
MAB363
CCL15/MIP-1 delta Antibody (52130) [Unconjugated]
Species: Hu
Applications: WB, B/N, ELISA(Cap)

Publications for CCL15/MIP-1 delta Antibody (MAB363)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Antibody Array Development, ICC, IHC-Fr.


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(1)
ICC
(1)
IHC-Fr
(1)
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(3)
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Bioinformatics

Gene Symbol CCL15
Uniprot