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Cathepsin X/Z/P Antibody [Unconjugated]

 (15 Publications)
 
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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/ Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF934) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Cathepsin X/Z/P at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, RAW 264.7 mouse monocyte/macrophage cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of ...read more
Cathepsin X/Z/P was detected in immersion fixed paraffin-embedded sections of normal human breast and breast cancer tissue using Goat Anti-Human/Mouse/Rat Cathepsin X/Z/P Antigen Affinity-purified Polyclonal Antibody ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin X/Z/P at approximately 36 kDa (as indicated) using 5 µg/mL ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF934
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Catalog# & Formulation Size Price
Carrier Free
AF934-SP
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Cathepsin X/Z/P Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Cathepsin X/Z/P
Ala21-Val303 (Gly23Val, Ser48Thr)
Accession # Q9UBR2
Specificity
Detects human Cathepsin X/Z/P in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CTSZ
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF934 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin X/Z/P Antibody [Unconjugated]

  • Cathepsin P
  • Cathepsin X/Z/P
  • Cathepsin Z
  • CTSX
  • CTSZ

Background

Cathepsin X (also known as Cathepsin Z and P) is a cysteine protease of the papain family (1‑5). Compared to other members of the papain family, Cathepsin X has a short proregion and unique insertions. The cysteine residue in the proregion forms a covalent and reversible bond with the active site cysteine residue (6). Acting as a carboxypeptidase, Cathepsin X displays a unique specificity (7‑10). It is ubiquitously expressed in human tissues and conserved in other species such as mouse, nematode and echiuran. The nematode enzyme is apparently involved in molting of third stage larvae (11).

  1. Nagler, D.K. and R. Menard (1998) FEBS Lett. 434:135.
  2. Santamaria, I. et al. (1998) J. Biol. Chem. 273:16816.
  3. Deussing, J. et al. (2000) Biochim. Biophys. Acta 1491:93.
  4. Pungercar, J. and G. Ivanovski (2000) Pflugers Arch. Eur. J. Physiol. 439:R116.
  5. Pungercar, J. et al. (2000) Pflugers Arch. Eur. J. Physiol. 439:R119.
  6. Sivaraman, J. et al. (2000) J. Mol. Biol. 295:935.
  7. Menard, R. et al. (2001) Biol. Chem. 382:839.
  8. Therrien, C. et al. (2001) Biochemistry 40:2702.
  9. Klemencic, I. et al. (2000) Eur. J. Biochem. 267:5404.
  10. Guncar, G. et al. (2000) Structure Fold Des. 8:305.
  11. Lustigman, S. et al. (1996) J. Biol. Chem. 271:30181.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cathepsin X/Z/P Antibody (AF934)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: ELISA Development, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(1)
ICC
(3)
IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
Western Blot
(2)
All Applications
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Human
(7)
Mouse
(1)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF934 Applications Species
Calcagni' A, Staiano L, Zampelli N et al. Loss of the batten disease protein CLN3 leads to mis-trafficking of M6PR and defective autophagic-lysosomal reformation Nature communications 2023-07-03 [PMID: 37400440]
A Hertel, LM Alves, H Dutz, G Tascher, F Bonn, M Kaulich, I Dikic, S Eimer, F Steinberg, A Bremm USP32-regulated LAMTOR1 ubiquitination impacts mTORC1 activation and autophagy induction Cell Reports, 2022-12-06;41(10):111653. 2022-12-06 [PMID: 36476874] (ICC, Western Blot, Human) ICC, Western Blot Human
Anja Pišlar, Biljana Božić Nedeljković, Mina Perić, Tanja Jakoš, Nace Zidar, Janko Kos Cysteine Peptidase Cathepsin X as a Therapeutic Target for Simultaneous TLR3/4-mediated Microglia Activation Molecular Neurobiology 2022-04-01 [PMID: 35066760]
Tanja Jakoš, Mateja Prunk, Anja Pišlar, Janko Kos Cathepsin X Activity Does Not Affect NK-Target Cell Synapse but Is Rather Distributed to Cytotoxic Granules International Journal of Molecular Sciences 2021-12-16 [PMID: 34948293]
Anja Pišlar, Larisa Tratnjek, Gordana Glavan, Nace Zidar, Marko Živin, Janko Kos Neuroinflammation-Induced Upregulation of Glial Cathepsin X Expression and Activity in vivo Frontiers in Molecular Neuroscience 2020-11-20 [PMID: 33328882]
Oliver B. Davis, Hijai R. Shin, Chun-Yan Lim, Emma Y. Wu, Matthew Kukurugya, Claire F. Maher et al. NPC1-mTORC1 Signaling Couples Cholesterol Sensing to Organelle Homeostasis and Is a Targetable Pathway in Niemann-Pick Type C Developmental Cell 2021-02-01 [PMID: 33308480]
Anja Pišlar, Larisa Tratnjek, Gordana Glavan, Marko Živin, Janko Kos Upregulation of Cysteine Protease Cathepsin X in the 6-Hydroxydopamine Model of Parkinson’s Disease Frontiers in Molecular Neuroscience 2018-11-02 [PMID: 30450037]
Barbara Breznik, Clara Limback, Andrej Porcnik, Andrej Blejec, Miha Koprivnikar Krajnc, Roman Bosnjak et al. Localization patterns of cathepsins K and X and their predictive value in glioblastoma Radiology and Oncology 2018-10-18 [PMID: 30367810]
Y Aiba, K Harada, M Ito, T Suematsu, S Aishima, Y Hitomi, N Nishida, M Kawashima, M Takatsuki, S Eguchi, S Shimoda, H Nakamura, A Komori, S Abiru, S Nagaoka, K Migita, H Yatsuhashi, K Tokunaga, M Nakamura Increased expression and altered localization of cathepsin Z are associated with progression to jaundice stage in primary biliary cholangitis Sci Rep, 2018-08-07;8(1):11808. 2018-08-07 [PMID: 30087368] (ICC, IHC-P, Human) ICC, IHC-P Human
P Anja, J Anahid, K Janko Cysteine cathepsins: Their biological and molecular significance in cancer stem cells Semin. Cancer Biol., 2018-07-29;0(0):. 2018-07-29 [PMID: 30063965] (ICC, Human) ICC Human
Show All 15 Publications.

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Bioinformatics

Gene Symbol CTSZ
Uniprot