CARD12 Antibody

Ipaf (also known as Clan/CARD12) is a CARD domain containing protein. CARD (caspase-associated recruitment domain) proteins are key regulators of cell death, cell survival and cytokine production (reviewed in Damiano and Reed, 2004). In general CARD proteins are implicated in host defense against infection, environmental stress or cellular damage. CARD domains are found in the N-terminal pro-domains of certain caspases, a family of apoptotic and pro-inflammatory proteases, as well as in a diversity of other proteins including Ipaf/Clan/CARD12. CARD domains are homotypic protein interaction motifs that enable networks of proteins to communicate via CARD-CARD interactions. There are at least three major signaling pathways in which CARD proteins act: (1) Regulation of caspase activation in the context of apoptosis (2) Regulation of caspase activation in the context of inflammation (3) Regaultion of NF-kB activation in the context of innate or adaptive immune responses. As there is significant crosstalk between pathways that lead to caspase-mediated apoptosis or inflammation and pathways that result in NF-kB activation, it is logical that similar protein modules such as CARD domains are found repeatedly in proteins from all three pathways. Ipaf plays a role in regulating caspase-1 activity, which in turn mediates the maturation of inflammatory cytokines IL-1b and IL-18 (reviewed in Lu et al, 2005). In transfected cells, Ipaf has been shown to directly interact with procaspase-1 and induce proteolytic activation of procaspase-1 in transfected cells. On the flip side, macrophages from IPAF deficient mice failed to activate caspase-1 in response to Salmonella typhimurium infection underscoring the importance of IPAF in vivo. IPAF also interact with the pro-apoptotic adaptor protein ASC and co-expression of IPAF with ASC has been shown to induce NF-kB activation and apoptosis.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IP, WB.

Showing Publications 1 - 6 of 6.

Publications using NB100-56142	Applications	Species
Ivarsson J, Ferrara F, Vallese A et al. Comparison of Pollutant Effects on Cutaneous Inflammasomes Activation International Journal of Molecular Sciences 2023-11-23 [PMID: 38068996]
Akhter A, Caution K, Abu Khweek A, Tazi M, Abdulrahman BA, Abdelaziz DH, Voss OH, Doseff AI, Hassan H, Azad AK, Schlesinger LS, Wewers MD, Gavrilin MA, Amer AO. Caspase-11 Promotes the Fusion of Phagosomes Harboring Pathogenic Bacteria with Lysosomes by Modulating Actin Polymerization. Immunity. 2012-05-31 [PMID: 22658523] (WB, Mouse)	WB	Mouse
Singatulina AS, Hamon L, Sukhanova MV et al. Leishmania targets the macrophage epigenome and dampens the NF- kappa B/NLRP3-mediated inflammatory response bioRxiv (WB, Human)	WB	Human
Wen S, Deng F, Li L et al. VX-765 ameliorates renal injury and fibrosis in diabetes by regulating caspase-1-mediated pyroptosis and inflammation Journal of diabetes investigation 2021-09-08 [PMID: 34494385] (WB, IP, Human, Mouse)	WB, IP	Human, Mouse
Kim H, Seo Js, Lee Sy et Al. AIM2 inflammasome contributes to brain injury and chronic post-stroke cognitive impairment in mice Brain Behav. Immun. 2020-03-19 [PMID: 32201254] (WB, Mouse)	WB	Mouse
Lecoeur H, Prina E, Rosazza T et al. Targeting Macrophage Histone H3 Modification as a Leishmania Strategy to Dampen the NF-kappa B/NLRP3-Mediated Inflammatory Response Cell Rep 2020-02-11 [PMID: 32049017] (WB, Mouse)	WB	Mouse

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	WB	Mouse	05/29/2024	View Summary Application Western Blot Sample Tested Mouse Spleen Species Mouse Lot AR91-091613-02

Array NB100-56142

• CARD12 Antibodies
• CARD12 Lysate
• CARD12 Proteins