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Mouse Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric)

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ELISA: Mouse Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric) [NBP3-06962] - Samples were spiked with high concentrations of Mouse Carboxypeptidase B2/CPB2 and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Mouse Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric) [NBP3-06962] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL

Order Details

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Mouse Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse Carboxypeptidase B2/CPB2 in samples. No significant cross-reactivity or interference between Mouse Carboxypeptidase B2/CPB2 and analogues was observed.
Standard Curve Range
0.78 - 50 ng/mL
Sensitivity
0.47 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
%CV < 4.97
Intra-Assay
%CV < 5.21
Spike Recovery
87 - 111%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CPB2

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse Carboxypeptidase B2/CPB2 ELISA Kit (Colorimetric)

  • carboxypeptidase B2 (plasma)
  • carboxypeptidase B2 (plasma, carboxypeptidase U)
  • Carboxypeptidase B2
  • Carboxypeptidase U
  • CPB2
  • CPU
  • CPUEC 3.4.17.20
  • EC 3.4.17
  • PCPB
  • Plasma carboxypeptidase B
  • TAFI
  • TAFIcarboxypeptidase B-like protein
  • Thrombin-activable fibrinolysis inhibitor
  • thrombin-activatable fibrinolysis inhibitor

Background

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Available sequence data analyses indicate splice variants that encode different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CPB2