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Canine IL-6 Quantikine ELISA Kit

 (17 Publications)
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Summary
Reactivity CaSpecies Glossary
Applications ELISA
Conjugate
HRP

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Canine IL-6 Quantikine ELISA Kit Summary

Background
The Quantikine Canine IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural canine IL-6 sho...wed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring canine IL-6.
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Specificity
Natural and recombinant canine IL-6
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL6

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using CA6000.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Canine IL-6 Quantikine ELISA Kit

  • B-cell differentiation factor
  • B-cell stimulatory factor 2
  • BSF2
  • BSF-2
  • CDF
  • CTL differentiation factor
  • HSF
  • hybridoma growth factor
  • IFNB2
  • IFN-beta-2
  • IL6
  • IL-6
  • Interferon beta-2
  • interleukin 6 (interferon, beta 2)
  • interleukin BSF-2
  • interleukin-6
  • MGI-2A

Background

Interleukin 6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature canine IL-6 is 187 amino acids (aa) in length and shares 58%, 37%, 39%, and 76% aa sequence identity with human, mouse, rat, and feline IL-6, respectively. Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells (2, 6-28). IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids (2). Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery (29-33). 
IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha , triggering IL-6 R alpha association with gp130 and gp130 dimerization (34). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (35). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130- expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 36). 
IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis (2, 5). IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity (1). It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis (1, 2). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of memory B cells and plasma cells (37). 

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Canine IL-6
Quantikine ELISA Kit
CA6000
Canine IL-6 Quantikine ELISA Kit
Species: Ca
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-6 (CA6000)(17)

We have publications tested in 1 confirmed species: Canine.


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Canine
(17)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using CA6000 Applications Species
HD Humes, KD Aaronson, DA Buffington, HN Sabbah, AJ Westover, LT Yessayan, B Szamosfalv, FD Pagani Translation of immunomodulatory therapy to treat chronic heart failure: Preclinical studies to first in human PLoS ONE, 2023-04-06;18(4):e0273138. 2023-04-06 [PMID: 37023139] (Canine) Canine
HD Humes, KD Aaronson, DA Buffington, HN Sabbah, AJ Westover, LT Yessayan, B Szamosfalv, FD Pagani Translation of immunomodulatory therapy to treat chronic heart failure: Preclinical studies to first in human PLoS ONE, 2023;18(4):e0273138. 2023 [PMID: 37023139] (Canine) Canine
E Razzuoli, CG De Ciucis, B Chirullo, K Varello, R Zoccola, L Guardone, P Petrucci, D Rubini, E Bozzetta, M Goria, F Fruscione, P Modesto Molecular Characterization of CF33 Canine Cell Line and Evaluation of Its Ability to Respond against Infective Stressors in Sight of Anticancer Approaches Veterinary sciences, 2022-10-02;9(10):. 2022-10-02 [PMID: 36288156] (Canine) Canine
S Kim, YS Hyun, HT Park, MK Shin, HS Yoo Mycobacterium intracellulare induces a Th17 immune response via M1-like macrophage polarization in canine peripheral blood mononuclear cells Scientific Reports, 2022-07-12;12(1):11818. 2022-07-12 [PMID: 35821058] (Canine) Canine
A Cheney, AD Woolcock, A Mukhopadhy, D Knapp, GE Moore Interleukin-6 and thrombopoietin concentrations in dogs with carcinoma with and without thrombocytosis Journal of veterinary internal medicine, 2021-12-08;0(0):. 2021-12-08 [PMID: 34881459] (Canine) Canine
R Nivy, N Chaim, E Hanael, GA Sutton, Y Bruchim, I Aroch, G Segev Prospective evaluation of 5 urinary biomarkers as predictors of acute kidney injury in nonazotemic, hospitalized dogs Journal of veterinary internal medicine, 2021-11-05;0(0):. 2021-11-05 [PMID: 34738653] (Canine) Canine
E Gugliandol, P Licata, AF Peritore, R Siracusa, R D'Amico, M Cordaro, R Fusco, D Impellizze, R Di Paola, S Cuzzocrea, R Crupi, CD Interlandi Effect of Cannabidiol (CBD) on Canine Inflammatory Response: An Ex Vivo Study on LPS Stimulated Whole Blood Veterinary sciences, 2021-09-04;8(9):. 2021-09-04 [PMID: 34564578] (Canine) Canine
A Nemec Svet, B Verk, N ?ebulj-Kad, J Salobir, V Rezar, A Domanjko P Inflammation and its association with oxidative stress in dogs with heart failure Bmc Veterinary Research, 2021-04-26;17(1):176. 2021-04-26 [PMID: 33902566] (Canine) Canine
H Suzuki, N Oshima, T Watari Effect of modified ultrafiltration on cytokines and hemoconcentration in dogs undergoing cardiopulmonary bypass J Vet Med Sci, 2020-10-05;0(0):. 2020-10-05 [PMID: 33012753] (Canine) Canine
E Lee, JH Choi, HJ Jeong, SG Hwang, S Lee, JW Oh Hematologic and serologic status of military working dogs given standard diet containing natural botanical supplements Toxicol Rep, 2018-03-08;5(0):343-347. 2018-03-08 [PMID: 29854603] (Canine) Canine
Show All 17 Publications.

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Product General Protocols

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FAQs for IL-6 (CA6000). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
    • We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.
Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.
The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein ...  Read full blog post.
The use of a GFP antibody for research applications in transgenic C. elegans, GFP tagged yeast and porcine model
GFP, or green fluorescent protein, is a chemiluminescent protein derived from Aequorea jellyfish that was first discovered by Osamu Shimomura.  It was soon after established that the emission spectra of GFP was right around 509nm, or the ultraviol...  Read full blog post.
VEGF: Vascular endothelial growth factor
VEGF is homodimeric, disulfide-linked glycoprotein cytokine that serves as the ligand for FLT1 (VEGFR-1 receptor) and FLK1 (VEGFR-2 receptor) tyrosine kinases. It is a key modulator of physiological angiogenesis, vasculogenesis, and endothelial cell g...  Read full blog post.
TLR2 - I can recognize many foreign pathogens!
TLR2 is a member of the broad family of Toll-like receptors (TLR) that play an important role in pathogen recognition and innate immunity. TLRs, like other pattern recognition receptors (PRRs), recognize endogenous ligands released during cell or tiss...  Read full blog post.
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Bioinformatics

Gene Symbol IL6