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Calretinin Antibody (CL12838) - Azide and BSA Free

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Staining of human cerebellum shows moderate to strong cytoplasmic positivity in cells in granular layer.
Staining of human cerebellum shows moderate to strong cytoplasmic positivity in cells in granular layer.
Orthogonal Strategies: Analysis in human cerebral cortex and liver tissues using NBP3-44168 antibody. Corresponding Calretinin RNA-seq data are presented for the same tissues.
Staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neurons.
Staining of human liver shows no positivity in hepatocytes as expected.
Staining of human ovarian cancer (granular cell carcinoma) shows moderate cytoplasmic positivity in tumor cells.
Staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.
Staining of mouse brain shows strong cytoplasmic positivity in a subset of neurons.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC
Clone
CL12838
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH
Validated by:
       

Orthogonal Strategies

  

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-18561AF350
Alexa Fluor 405
NBP3-18561AF405
Alexa Fluor 488
NBP3-18561AF488
Alexa Fluor 532
NBP3-18561AF532
Alexa Fluor 594
NBP3-18561AF594
Alexa Fluor 647
NBP3-18561AF647
Alexa Fluor 700
NBP3-18561AF700
Alexa Fluor 750
NBP3-18561AF750
Allophycocyanin
NBP3-18561APC
Biotin
NBP3-18561B
CoraFluor 1
NBP3-18561CL1
DyLight 350
NBP3-18561UV
DyLight 405
NBP3-18561V
DyLight 488
NBP3-18561G
DyLight 550
NBP3-18561R
DyLight 594
NBP3-18561DL594
DyLight 650
NBP3-18561C
DyLight 680
NBP3-18561FR
DyLight 755
NBP3-18561IR
FITC
NBP3-18561F
HRP
NBP3-18561H
Janelia Fluor 525
NBP3-18561JF525
Janelia Fluor 549
NBP3-18561JF549
Janelia Fluor 646
NBP3-18561JF646
Janelia Fluor 669
NBP3-18561JF669
PE
NBP3-18561PE
PerCP
NBP3-18561PCP
Unconjugated
NBP3-18561-100ul
mFluor Violet 450 SE
NBP3-18561MFV450
mFluor Violet 500 SE
NBP3-18561MFV500
mFluor Violet 610 SE
NBP3-18561MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-18561-100ul
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Calretinin Antibody (CL12838) - Azide and BSA Free Summary

Immunogen
This antibody was generated using a recombinant protein sequence of P22676, with the exact immunogen sequence remaining proprietary.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CALB2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized in 5% Trehalose.
Preservative
0.02% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Calretinin Antibody (CL12838) - Azide and BSA Free

  • 29 kDa calbindin
  • CAB29
  • CAL2
  • CALB2
  • calbindin 2
  • calbindin 2, (29kD, calretinin)
  • calbindin 2, 29kDa (calretinin)
  • calbindin D29K
  • Calretinin
  • CR

Background

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP3-44168
Calretinin Antibody (CL12838) - Azide and BSA Free
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Bioinformatics

Gene Symbol CALB2