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Calmodulin Antibody (2D1) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ba, Bv, ChSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
2D1
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB120-2860AF350
Alexa Fluor 405
NB120-2860AF405
Alexa Fluor 488
NB120-2860AF488
Alexa Fluor 532
NB120-2860AF532
Alexa Fluor 594
NB120-2860AF594
Alexa Fluor 700
NB120-2860AF700
Alexa Fluor 750
NB120-2860AF750
Allophycocyanin
NB120-2860APC
Biotin
NB120-2860B
DyLight 350
NB120-2860UV
DyLight 405
NB120-2860V
DyLight 488
NB120-2860G
DyLight 550
NB120-2860R
DyLight 594
NB120-2860DL594
DyLight 650
NB120-2860C
DyLight 680
NB120-2860FR
DyLight 755
NB120-2860IR
FITC
NB120-2860F
HRP
NB120-2860H
Janelia Fluor 525
NB120-2860JF525
Janelia Fluor 669
NB120-2860JF669
PE
NB120-2860PE
PerCP
NB120-2860PCP
Unconjugated
NB120-2860
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB120-2860
BSA Free
NB120-2860
BSA Free
NB120-2860-50ul
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Calmodulin Antibody (2D1) [Alexa Fluor® 647] Summary

Immunogen
Calmodulin purified from Dictyostelium discoideum.
Specificity
This detects calmodulin. It does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CALM1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Publications
Read Publication using
NB120-2860AF647 in the following applications:

  • FC
    1 publication
  • IF
    1 publication

Reactivity Notes

E. coli. Chlamydomonas reinhardtii and Dictyostelium discoideum. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Calmodulin Antibody (2D1) [Alexa Fluor® 647]

  • CALM
  • CALM1
  • CALM2
  • CALM3
  • CALML2
  • calmodulin 1 (phosphorylase kinase, delta)
  • Calmodulin 1
  • calmodulin
  • CaM
  • CAM1
  • CAM2
  • CAM3
  • CAMB
  • CAMC
  • CAMI
  • CAMIII
  • CPVT4
  • DD132
  • PHKDCAM
  • phosphorylase kinase, delta subunit

Background

Calmoduin (CaM) is a calcium modulator protein and a transducer of calcium signals (1-2). Upon calcium binding, calmodulin undergoes conformational changes and binds and modulates a diverse array of proteins. Calcium-bound CaM (Ca2+-CaM) can assume a variety of shapes depending on the target (3). Ca2+-CaM binds many kinases, phosphatases, signaling proteins, and structural proteins affecting a wide variety of processes including neurotransmitter release, muscle contraction, metabolism, apoptosis, inflammation, membrane protein organization, and cytoskeleton movement (2, 4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Calmodulin
Antibody (2D1) [Alexa ...
NB120-2860AF647
Calmodulin Antibody (2D1) [Alexa Fluor® 647]
Species: Hu, Mu, Rt, Ba, Bv, Ch
Applications: WB, ELISA, Flow, ICC/IF, IHC

Publications for Calmodulin Antibody (NB120-2860AF647)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FC, IF.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calmodulin Antibody (NB120-2860AF647). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for an antibody that can bind an engineered calmodulin domain of a fluorescent biosensor. Can you provide me the amino acid sequence that your calmodulin antibodies were raised against so that I can compare them to the sequence I am working with? Can you please provide your sequence identity for this fragment: D Q L T E E Q I A E F K E A F S L F D K D G D G T I T T K E L G T V M R S L G Q N P T E A E L Q D M I N E V D A D G D G T I D F P E F L T M M A R K M W G T D S E E E I R E A F R V F D K D G N G Y I G A A E L R H V M A N L G E R L T D E E V D E M I R V A D I N G D G Q V S Y E E F V Q M M T A K
    • Please see this link to our available calmodulin antibodies. This sequences shares 94% similarity with human calmodulin and does not share 100% similarity with any known calmodulin sequence. Any of our calmodulin antibodies will be predicted to react with this sequence. Any of our polyclonal antibodies will be a good choice and chances are most of the monoclonal antibodies will cross-react as well although, unfortunately, there is no way to predict this with the monoclonals. NBP1-61548 would be my best recommendation for your assay and sequence.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CALM1