| Reactivity | HuSpecies Glossary |
| Applications | ICC/IF |
| Clonality | Polyclonal |
| Host | Sheep |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Immunogen | E. coli-derived recombinant human C2CD3 Met1-Asp109 Accession # Q4AC94 |
| Specificity | Detects human C2CD3 in direct ELISAs. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Sheep |
| Gene | C2CD3 |
| Purity Statement | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Sterile PBS to a final concentration of 0.2 mg/mL. |
C2CD3 (C2 domain-containing protein 3) is a 260 kDa (predicted) intracellular protein that apparently belongs to no known structural protein family. It is ubiquitously expressed, and plays a role in cilia formation and associated hedgehog signaling. In particular, C2CD3 is localized to the basal body, and appears to promote Gli3 proteolytic processing into a truncated transcriptional repressor. Human C2CD3 is 2353 amino acids (aa) in length. It contains two C2 domains (aa 1163-1289 and 1622-1728) that participate in Ca++-dependent protein-lipid and protein-protein interactions. Three utilized phosphorylation sites exist at Thr1130, Ser2114 and Ser 2132. There are multiple potential splice variants. One possesses a three aa substitution for aa 1961-2353, a second shows a deletion of aa 1364-1382 coupled to a 27 aa substitution for aa 1881-2353, a third contains a seven aa substitution for aa 1961-2353, and a fourth contains a 34 aa substitution for aa 1888-2353. Over aa 1-109, human C2CD3 shares 94% aa identity with mouse C2CD3.
Secondary Antibodies |
Isotype Controls |
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