![Western Blot: c-Rel Antibody [NB600-474] - All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.](http://images.novusbio.com/fullsize/c-Rel-Antibody-Western-Blot-NB600-474-img0005.jpg "Western Blot: c-Rel Antibody [NB600-474] - All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.")
| Reactivity | Hu, Mu(-)Species Glossary |
| Applications | WB, Simple Western, ELISA, GS |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This product was prepared from monospecific antiserum by delipidation and defibrination Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Immunogen | c-Rel peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). (Uniprot: Q04864) |
| Localization | Nuclear |
| Specificity | Anti-c-Rel may react non-specifically with other proteins. Control peptide will compete only with the specific reaction of antiserum with the c-Rel subunit. |
| Isotype | Serum |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | REL |
| Purity | Delipidation and Defibrination |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This product was tested by ELISA and by immunoblot. This product is suitable for the detection by immunoblot of human NFkB cRel at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . No reaction was observed against the analogous Mouse protein. This product was also tested in a gel supershift assay and found to be reactive against all cRel containing human NFkB complexes using 0.5 to 1.0 ul per assay. Simple Western Antibody Database for Simple Western validation: tested in HEK-/+UV, whole cell lysate; separated by charge, antibody dilution of 1:50. |
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| Control Peptide |
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| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Delipidation and Defibrination |
Secondary Antibodies |
Isotype Controls |
Research Areas for c-Rel Antibody (NB600-474)Find related products by research area.
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NFkB and p62 Both Activate and Regulate Inflammation Nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB) is a protein complex that regulates DNA transcription and is a critical regulator of cell survival. NFkB has long been known as a primer of inflammation, however researchers are ... Read full blog post. |
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RelA/NF-kB - A proinflammatory signaling pathway with roles in immunity and cancer The inflammatory response consists of a complex network of signaling pathways that regulate a diverse set of cytokines, growth factors, adhesion molecules, and transcription factors (1). Of the proinflammatory signaling pathways the NF-kB family is... Read full blog post. |
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Nuclear Factor kappa B Signaling in the Immune System Nuclear Factor kappa B (NFkB) binds to the kappa-beta site of the immunoglobulin kappa light chain gene enhancer. Thus NFkB has become one of the widely studied transcription factors in innate and adaptive immune responses. The NFkB family is composed... Read full blog post. |
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![Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.](http://images.novusbio.com/fullsize/nb600-474_rabbit-polyclonal-c-rel-antibody-255202313292313.jpg "Western blot of HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")