c-Fos Antibody

c-Fos is an intermediate early gene that is one of four members of the FOS family of activator protein-1 (AP-1) transcription factors, which also includes Fra-1, Fra-2, and FosB (1-3). Under the FOS gene, human c-Fos is synthesized as a protein of 308 amino acids (aa) with a basic leucine zipper (bZip) domain important for dimerization, and a basic domain for interacting with DNA, with a theoretical molecular weight of ~40.6 kDa (4,5). c-Fos can heterodimerize with members of the JUN family (c-Jun, JunB, and JunD) to form the active transcription factor AP-1 complex (3,5). AP-1 related proteins bind TPA-related responsive elements (TRE), cAMP responsive elements (CRE), and related sequences, with c-Fos:c-Jun dimers partial to TRE sites (5).

In response to stimuli, c-Fos, which is encoded by protooncogenes, has a role in cell proliferation, differentiation, and transformation (3,6). A variety of stimuli can increase c-Fos expression such as growth factors, proinflammatory cytokines, UV radiation, neurotransmitters, hormones, injury, and stress (1,6). c-Fos has long been used as a marker for neuronal activity and is associated with neural and behavioral responses following stimuli (1-3, 6-7). Mouse studies have revealed that c-Fos is important for efficient neurogenesis and cortical development (3). Additionally, c-Fos signal can be used as a molecular marker for learning and memory, such as recognition and fear (2,7). Studies have found that repeated positive stimuli result in increased Fos expression while, conversely, repeated negative value stimuli are indicated by decreased signal (7). Intermediate early genes have also been implicated in neuropsychiatric disorders including showing altered c-Fos expression in a schizophrenia animal model (2). Furthermore, antipsychotics and antidepressants are both capable of impacting c-Fos expression (2).

References

1. Kovacs K. J. (1998). c-Fos as a transcription factor: a stressful (re)view from a functional map. Neurochemistry International. https://doi.org/10.1016/s0197-0186(98)00023-0

2. Gallo, F. T., Katche, C., Morici, J. F., Medina, J. H., & Weisstaub, N. V. (2018). Immediate Early Genes, Memory and Psychiatric Disorders: Focus on c-Fos, Egr1 and Arc. Frontiers in Behavioral Neuroscience. https://doi.org/10.3389/fnbeh.2018.00079

3. Velazquez, F. N., Caputto, B. L., & Boussin, F. D. (2015). c-Fos importance for brain development. Aging. https://doi.org/10.18632/aging.100862

4. Uniprot (P01100)

5. Wu, Z., Nicoll, M., & Ingham, R. J. (2021). AP-1 family transcription factors: a diverse family of proteins that regulate varied cellular activities in classical hodgkin lymphoma and ALK+ ALCL. Experimental Hematology & Oncology. https://doi.org/10.1186/s40164-020-00197-9

6. Shaulian, E., & Karin, M. (2001). AP-1 in cell proliferation and survival. Oncogene. https://doi.org/10.1038/sj.onc.1204383

7. Chung L. (2015). A Brief Introduction to the Transduction of Neural Activity into Fos Signal. Development & Reproduction. https://doi.org/10.12717/DR.2015.19.2.061

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: IF/IHC, IHC-Fr.

Showing Publications 1 - 7 of 7.

Publications using NB110-75039	Applications	Species
Katherine Blackmore, Claire J. Houchen, Hayk Simonyan, Hovhannes Arestakesyan, Alyssa K. Stark, Samantha A. Dow, Han Rae Kim, Jin Kwon Jeong, Anastas Popratiloff, Colin N. Young A forebrain-hypothalamic ER stress driven circuit mediates hepatic steatosis during obesity Molecular Metabolism 2023-12-21 [PMID: 38141847]
Dongxiao QU, Yiqin GE, Limin Z et al. Efficacy and mechanisms of Dingxian pill combined with valproic acid on pentylenetetrazol-induced chronic epilepsy in rats Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 2023-04-01 [PMID: 36994516] (IHC-Fr, Rat)	IHC-Fr	Rat
Cuddy SR, Schinlever AR, Dochnal S, et al. Neuronal hyperexcitability is a DLK-dependent trigger of herpes simplex virus reactivation that can be induced by IL-1 eLife 2020-12-22 [PMID: 33350386]
Zhu Y, Zhang S, Shen M et al. Anticonvulsant Effects of Dingxian Pill in Pentylenetetrazol-Kindled Rats Evid Based Complement Alternat Med. 2019-03-18 [PMID: 31011358] (IF/IHC, Rat)	IF/IHC	Rat
Tang Y, Liu L, Xu D et al. Interaction between astrocytic colony stimulating factor and its receptor on microglia mediates central sensitization and behavioral hypersensitivity in chronic post ischemic pain model. Brain Behav Immun 2018-02-01 [PMID: 29080683] (Rat)		Rat
Blackmore K Forebrain-Hypothalamic Circuits, Endoplasmic Reticulum Stress, and Sexual Dimorphism in Hepatic Steatosis During Obesity Thesis Jul 13 1905
Blackmore K Forebrain-Hypothalamic Circuits, Endoplasmic Reticulum Stress, and Sexual Dimorphism in Hepatic Steatosis During Obesity Thesis 1905-07-13

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Hayk Simonyan	ICC	Mouse	05/23/2018	View Summary Application Immunocytochemistry Sample Tested Mouse brain Species Mouse Lot rb2500-140415-ws

Array NB110-75039

• c-Fos Antibodies
• c-Fos ELISA Kits
• c-Fos Lysate
• c-Fos Proteins