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BubR1 Antibody - N-terminal

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Western Blot: BubR1 Antibody - N-terminal [NBP3-30806] - Immunoprecipitation/Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.
Western Blot: BubR1 Antibody - N-terminal [NBP3-30806] - Immunoprecipitation/Western blot analysis using Bub1b-NT antibody on NIH/3T3 cells synchronized to obtain mostly mitotic cells as determined by flowcytometry.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated

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BubR1 Antibody - N-terminal Summary

Immunogen
Recombinant protein corresponding to amino acids 10-190 of the mouse BubR1 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
BUB1B
Purity
Ammonium sulfate precipitation
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunoprecipitation : 1-10 ug/ml
  • Western Blot : 1-10 ug/ml
Application Notes
Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot analysis. Detects a 130 kDa band..

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Phosphate buffered saline
Preservative
0.08% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for BubR1 Antibody - N-terminal

  • Bub1A
  • BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • EC 2.7.11.1
  • hBUBR1
  • MAD3/BUB1-related protein kinase
  • MAD3LBUB1beta
  • Mitotic checkpoint kinase MAD3L
  • Protein SSK1
  • SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.

BubR1 antibodies are useful tools for cancer research and cell division studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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BubR1 Antibody -
N-terminal
NBP3-30806
BubR1 Antibody - N-terminal
Species: Mu
Applications: WB, IHC, IP

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Bioinformatics

Gene Symbol BUB1B