![Western Blot: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Western-Blot-NBP2-02472-img0002.jpg "Western Blot: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI3A2 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Full length human recombinant protein of human BTK (NP_000052) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | BTK |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 76.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunocytochemistry-Immunofluorescence-NBP2-02472-img0009.jpg "Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.")
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded prostate tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0008.jpg "Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded prostate tissue using anti-BTK mouse monoclonal antibody.")
![Flow Cytometry: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells transfected with either pCMV6-ENTRY BTK.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Flow-Cytometry-NBP2-02472-img0001.jpg "Flow Cytometry: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells transfected with either pCMV6-ENTRY BTK.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.")
![Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] - Immunofluorescent staining of HT29 cells using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunocytochemistry-Immunofluorescence-NBP2-02472-img0003.jpg "Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] - Immunofluorescent staining of HT29 cells using anti-BTK mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0004.jpg "Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BTK mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0005.jpg "Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BTK mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0006.jpg "Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-BTK mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Kidney tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0007.jpg "Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Kidney tissue using anti-BTK mouse monoclonal antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")