![Western Blot: BRD3 Antibody (BLR069G) [NBP2-77359] - Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] (NBP2-77359). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/BRD3-Antibody-BLR069G-Western-Blot-NBP2-77359-img0003.jpg "Western Blot: BRD3 Antibody (BLR069G) [NBP2-77359] - Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] (NBP2-77359). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | BLR069G |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Immunogen was a peptide representing a region between residue 676 and 726 of human Bromodomain-containing protein 3 using the numbering given in entry NP_031397.1 (Gene ID 8019). |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | BRD3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
| Storage | Store at 2 - 8 C / 1 year from date of receipt |
| Buffer | Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for BRD3 Antibody (NBP2-77359)Find related products by research area.
|
|
Understanding the relationship between NUT and BET proteins in NMC NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati... Read full blog post. |
|
NUT - A Protein Coding Gene The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | BRD3 |
![Immunocytochemistry: BRD3 Antibody (BLR069G) [NBP2-77359] - Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] (NBP2-77359). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/BRD3-Antibody-BLR069G-Immunocytochemistry-NBP2-77359-img0002.jpg "Immunocytochemistry: BRD3 Antibody (BLR069G) [NBP2-77359] - Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] (NBP2-77359). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.")
![Immunohistochemistry: BRD3 Antibody (BLR069G) [NBP2-77359] - Detection of human BRD3 FFPE colon carcinoma by IHC. Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] NBP2-77359. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/BRD3-Antibody-BLR069G-Immunohistochemistry-NBP2-77359-img0004.jpg "Immunohistochemistry: BRD3 Antibody (BLR069G) [NBP2-77359] - Detection of human BRD3 FFPE colon carcinoma by IHC. Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] NBP2-77359. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI (blue).")
![Immunohistochemistry: BRD3 Antibody (BLR069G) [NBP2-77359] - Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] (NBP2-77359). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/BRD3-Antibody-BLR069G-Immunohistochemistry-NBP2-77359-img0001.jpg "Immunohistochemistry: BRD3 Antibody (BLR069G) [NBP2-77359] - Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] (NBP2-77359). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.")
![Immunohistochemistry DNER Antibody [Unconjugated]](https://images.novusbio.com/images2/DNER_AF2254_Immunohistochemistry_18506.jpg)
![Immunocytochemistry/ Immunofluorescence DNER Antibody [Unconjugated]](https://images.novusbio.com/images/af2254_mouse-dner-affinity-purified-polyclonal-ab-41202412394943.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")