I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Name: Bovine FGF basic/FGF2/bFGF Protein
Brand: Novus Biologicals
SKU: 133-FB
Price: 453 USD

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Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our  NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	BvSpecies Glossary
Applications	Bioactivity

Catalog# & Formulation	Size	Price
Carrier Free 133-FB-025/CF

Details of Functionality	Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120. The ED₅₀ for this effect is 0.125-0.625 ng/mL.
Source	Bovine brain tissue-derived FGF basic/FGF2/bFGF protein
Protein/Peptide Type	Natural Proteins
Gene	FGF2
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note	<1.0 EU per 1 μg of the protein by the LAL method.

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl with BSA as a carrier protein.
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Bovine FGF basic/FGF2/bFGF Protein

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Publications using 133-FB	Applications	Species
Tsissios, G;Sallese, A;Raul Perez-Estrada, J;Tangeman, JA;Chen, W;Smucker, B;Ratvasky, SC;Grajales-Esquivel, E;Martinez, A;Visser, KJ;Araus, AJ;Wang, H;Simon, A;Yun, MH;Del Rio-Tsonis, K; Macrophages modulate fibrosis during newt lens regeneration bioRxiv : the preprint server for biology 2023-06-05 [PMID: 37333184] (In Vivo, Newt)	In Vivo	Newt
Tangeman J, Perez-Estrada J, Van Zeeland E, Liu L, Danciutiu A, Grajales-Esquivel E, Smucker B, Liang C, Del Rio-Tsonis K A Stage-Specific OTX2 Regulatory Network and Maturation-Associated Gene Programs Are Inherent Barriers to RPE Neural Competency. Front Cell Dev Biol, 2022-04-19;10(0):875155. 2022-04-19 [PMID: 35517508] (In Vivo, Chicken)	In Vivo	Chicken
RW Scott, M Arostegui, R Schweitzer, FMV Rossi, TM Underhill Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle Regeneration Cell Stem Cell, 2019-12-05;25(6):797-813.e9. 2019-12-05 [PMID: 31809738] (Cell Culture, Mouse)	Cell Culture	Mouse
X Gong, W Liu, L Wu, Z Ma, Y Wang, S Yu, J Zhang, H Xie, G Wei, F Ma, L Lu, L Chen Transcriptional repressor GATA binding 1-mediated repression of SRY-box 2 expression suppresses cancer stem cell functions and tumor initiation J. Biol. Chem., 2018-10-12;0(0):. 2018-10-12 [PMID: 30315105] (Bioassay, Human)	Bioassay	Human
BA Boswell, A Korol, JA West-Mays, LS Musil Dual function of TGF? in lens epithelial cell fate: implications for secondary cataract Mol. Biol. Cell, 2017-02-16;0(0):. 2017-02-16 [PMID: 28209733] (Bioassay, Chicken)	Bioassay	Chicken
Tsai SY, Bouwman BA, Ang YS, Kim SJ, Lee DF, Lemischka IR, Rendl M Single transcription factor reprogramming of hair follicle dermal papilla cells to induced pluripotent stem cells. Stem Cells, 2011-06-01;29(6):964-71. 2011-06-01 [PMID: 21563278] (Bioassay, Mouse)	Bioassay	Mouse
Boswell BA, Overbeek PA, Musil LS Essential role of BMPs in FGF-induced secondary lens fiber differentiation. Dev. Biol., 2008-09-18;324(2):202-12. 2008-09-18 [PMID: 18848538] (Bioassay, Chicken)	Bioassay	Chicken
Zheng J, Wen Y, Song Y, Wang K, Chen DB, Magness RR Activation of multiple signaling pathways is critical for fibroblast growth factor 2- and vascular endothelial growth factor-stimulated ovine fetoplacental endothelial cell proliferation. Biol. Reprod., 2007-09-26;78(1):143-50. 2007-09-26 [PMID: 17901071] (Bioassay, Ovine)	Bioassay	Ovine
Horst AK, Ito WD, Dabelstein J, Schumacher U, Sander H, Turbide C, Brummer J, Meinertz T, Beauchemin N, Wagener C Carcinoembryonic antigen-related cell adhesion molecule 1 modulates vascular remodeling in vitro and in vivo. J. Clin. Invest., 2006-05-04;116(6):1596-605. 2006-05-04 [PMID: 16680193] (In Vivo, Mouse)	In Vivo	Mouse
Collombet JM, Four E, Burckhart MF, Masqueliez C, Bernabe D, Baubichon D, Herodin F, Lallement G Effect of cytokine treatment on the neurogenesis process in the brain of soman-poisoned mice. Toxicology, 2005-05-15;210(1):9-23. 2005-05-15 [PMID: 15804454] (In Vivo, Mouse)	In Vivo	Mouse