Home » Bone marrow stromal cell antigen 1/CD157 » Bone marrow stromal cell antigen 1/CD157 Lysates » Bone marrow stromal cell antigen 1/CD157 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

Bone marrow stromal cell antigen 1/CD157 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: Bone marrow stromal cell antigen 1/CD157 Overexpression Lysate [NBL1-08039] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Bone marrow stromal cell antigen 1.
Western Blot: Bone marrow stromal cell antigen 1/CD157 Overexpression Lysate [NBL1-08039] - Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Bone marrow stromal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Bone marrow stromal cell antigen 1/CD157 Overexpression Lysate Summary

Description

Bone marrow stromal cell antigen 1/CD157 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC204151

Accession#: NM_004334

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
BST1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
35.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for Bone marrow stromal cell antigen 1/CD157 Overexpression Lysate

  • ADP-ribosyl cyclase 2
  • Bone marrow stromal antigen 1
  • bone marrow stromal cell antigen 1
  • BP-3
  • BST1
  • BST-1
  • cADPr hydrolase 2
  • CD157 antigen
  • CD157
  • Cyclic ADP-ribose hydrolase 2
  • EC 3.2.2.5
  • NAD(+) nucleosidase

Background

Bone marrow stromal cell antigen-1 is a stromal cell line-derived glycosylphosphatidylinositol-anchored molecule that facilitates pre-B-cell growth. The deduced amino acid sequence exhibits 33% similarity with CD38. BST1 expression is enhanced in bone marrow stromal cell lines derived from patients with rheumatoid arthritis. The polyclonal B-cell abnormalities in rheumatoid arthritis may be, at least in part, attributed to BST1 overexpression in the stromal cell population. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NOD2 Antibody
(2D9) - BSA Free
NB100-524
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Flow Cytometry NOD2 Antibody (2D9) - BSA Free
Immunohistochemistry-Frozen NOD2 Antibody (2D9) - BSA Free
(1 Review)
(26 Publications)
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
HLA DQ/DR/DP
Antibody (HLA-Pan/296 ...
NBP2-79843
Protein Array HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Western Blot HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
CTLA-4
[Unconjugated]
7268-CT
Bioactivity CTLA-4 [Unconjugated]
(3 Publications)
Species: Hu
Applications: BA
CD38 Antibody
(1G7F4) - Azide Free
NBP2-25265
Immunohistochemistry-Paraffin CD38 Antibody (1G7F4) - Azide Free
Western Blot CD38 Antibody (1G7F4) - Azide Free
Simple Western CD38 Antibody (1G7F4) - Azide Free
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
alpha-Synuclein
Antibody
NBP2-15365
Immunohistochemistry-Frozen alpha-Synuclein Antibody
Immunohistochemistry-Frozen alpha-Synuclein Antibody
Immunocytochemistry/ Immunofluorescence alpha-Synuclein Antibody
(1 Review)
(12 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, S-ELISA, WB
LRRK2 Antibody -
BSA Free
NB300-268
Flow Cytometry LRRK2 Antibody - BSA Free
Immunohistochemistry-Paraffin LRRK2 Antibody - BSA Free
Flow Cytometry LRRK2 Antibody - BSA Free
(1 Review)
(46 Publications)
Species: Bv, Ce, Hu, I, Mu, Pl
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO, WB
GNPDA1 Antibody
NBP2-24653
Western Blot GNPDA1 Antibody
Immunohistochemistry-Paraffin GNPDA1 Antibody
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Glucose 6
phosphate isomerase A ...
NBP2-02541
Western Blot Glucose 6 phosphate isomerase Antibody (OTI2D2)
Immunohistochemistry-Paraffin Glucose 6 phosphate isomerase Antibody (OTI2D2)
Immunohistochemistry-Paraffin Glucose 6 phosphate isomerase Antibody (OTI2D2)
(1 Publication)
Species: Hu, Pm, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Exosome component
10 Antibody
NBP1-32870
Western Blot Exosome component 10 Antibody
Immunohistochemistry-Paraffin Exosome component 10 Antibody
Western Blot Exosome component 10 Antibody
(2 Reviews)
(3 Publications)
Species: Hu
Applications: IHC, IHC-P, IP, KD, WB
CD117/c-kit
Antibody [Unconjugate
AF1356
Simple Western CD117/c-kit Antibody [Unconjugated]
Western Blot CD117/c-kit Antibody [Unconjugated]
Immunohistochemistry CD117/c-kit Antibody [Unconjugated]
(4 Reviews)
(116 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, IHC, Simple Western, WB
GAK Antibody
(706306)
MAB6918
Western Blot GAK Antibody (706306)
(2 Publications)
Species: Hu
Applications: WB
Tau Antibody (2E9)
NBP2-25162
Immunocytochemistry/ Immunofluorescence Tau Antibody (2E9)
Western Blot Tau Antibody (2E9)
Immunocytochemistry/ Immunofluorescence Tau Antibody (2E9)
(5 Publications)
Species: Bv, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, PLA, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(811 Publications)
Species: Hu
Applications: BA
ICAM-1/CD54
Antibody [Unconjugate
AF796
Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
Western Blot ICAM-1/CD54 Antibody [Unconjugated]
(6 Reviews)
(88 Publications)
Species: Mu
Applications: AdBlk, IHC, WB
Integrin beta
1/CD29 Antibody (P5D2 ...
MAB17781
Flow Cytometry Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]
Immunocytochemistry Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]
Flow Cytometry Integrin beta 1/CD29 Antibody (P5D2) [Unconjugated]
(33 Publications)
Species: Hu
Applications: Block, CyTOF-ready, Flow, ICC, IP, KO

TRANCE/TNFSF11/RANK L
462-TEC
SDS-PAGE TRANCE/TNFSF11/RANK L [Unconjugated]
Bioactivity TRANCE/TNFSF11/RANK L [Unconjugated]
(90 Publications)
Species: Mu
Applications: BA
Oct-3/4 Antibody
[Unconjugated]
AF1759
Chromatin Immunoprecipitation (ChIP) Oct-3/4 Antibody [Unconjugated]
Immunocytochemistry Oct-3/4 Antibody [Unconjugated]
Simple Western Oct-3/4 Antibody [Unconjugated]
(77 Publications)
Species: Hu, Mu
Applications: ChIP, ICC, IP, Simple Western, WB
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(656 Publications)
Species: Mu
Applications: ELISA
MCAM/CD146
Antibody [Unconjugate
AF932
Immunohistochemistry MCAM/CD146 Antibody [Unconjugated]
Western Blot MCAM/CD146 Antibody [Unconjugated]
Immunohistochemistry MCAM/CD146 Antibody [Unconjugated]
(8 Publications)
Species: Hu
Applications: ICC, IHC, Simple Western, WB
Bone marrow
stromal cell antigen ...
NBL1-08039
Bone marrow stromal cell antigen 1/CD157 Overexpression Lysate
Species: Hu
Applications: WB

Publications for Bone marrow stromal cell antigen 1/CD157 Lysate (NBL1-08039) (0)

There are no publications for Bone marrow stromal cell antigen 1/CD157 Lysate (NBL1-08039).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Bone marrow stromal cell antigen 1/CD157 Lysate (NBL1-08039) (0)

There are no reviews for Bone marrow stromal cell antigen 1/CD157 Lysate (NBL1-08039). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Bone marrow stromal cell antigen 1/CD157 Lysate (NBL1-08039) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Bone marrow stromal cell antigen 1/CD157 Products

Research Areas for Bone marrow stromal cell antigen 1/CD157 Lysate (NBL1-08039)

Find related products by research area.

Blogs on Bone marrow stromal cell antigen 1/CD157

There are no specific blogs for Bone marrow stromal cell antigen 1/CD157, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Bone marrow stromal cell antigen 1/CD157 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol BST1