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BLNK Antibody (OTI4C9) - Azide and BSA Free

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Western Blot: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BLNK.
Western Blot: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BLNK.
Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Carcinoma of Human kidney tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: BLNK Antibody (OTI4C9) - Azide and BSA Free [NBP2-71855] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI4C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71855AF350
Alexa Fluor 405
NBP2-71855AF405
Alexa Fluor 488
NBP2-71855AF488
Alexa Fluor 532
NBP2-71855AF532
Alexa Fluor 594
NBP2-71855AF594
Alexa Fluor 647
NBP2-71855AF647
Alexa Fluor 700
NBP2-71855AF700
Alexa Fluor 750
NBP2-71855AF750
Allophycocyanin
NBP2-71855APC
Biotin
NBP2-71855B
CoraFluor 1
NBP2-71855CL1
DyLight 350
NBP2-71855UV
DyLight 405
NBP2-71855V
DyLight 488
NBP2-71855G
DyLight 550
NBP2-71855R
DyLight 594
NBP2-71855DL594
DyLight 650
NBP2-71855C
DyLight 680
NBP2-71855FR
DyLight 755
NBP2-71855IR
FITC
NBP2-71855F
HRP
NBP2-71855H
Janelia Fluor 525
NBP2-71855JF525
Janelia Fluor 549
NBP2-71855JF549
Janelia Fluor 585
NBP2-71855JF585
Janelia Fluor 635
NBP2-71855JF635
Janelia Fluor 646
NBP2-71855JF646
Janelia Fluor 669
NBP2-71855JF669
PE
NBP2-71855PE
PerCP
NBP2-71855PCP
Unconjugated
NBP2-46577
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46577
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BLNK Antibody (OTI4C9) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 1-292 of human BLNK(NP_037446) produced in E.coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
BLNK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
50.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for BLNK Antibody (OTI4C9) - Azide and BSA Free

  • AGM4
  • B cell linker protein
  • BASH
  • B-cell adapter containing a SH2 domain protein
  • B-cell adapter containing a Src homology 2 domain protein
  • B-cell linker protein
  • B-cell linker
  • BLNK
  • BLNK-s
  • Cytoplasmic adapter protein
  • Ly57
  • MGC111051
  • SLP65
  • SLP-65BLNK-S
  • SLP65Ly57
  • Src homology 2 domain-containing leukocyte protein of 65 kDa

Background

B-cell linker protein (BLNK, also known as SLP65, BASH, and BCA) is a B cell adaptor that plays an important role in B cell development and in BCR-signal transduction. Since BLNK does not encode any intrinsic enzymatic activity, its function is to serve as a scaffold for assembling macromolecular complexes which includes enzymes (PLC gamma, Vav and Btk) and additional linker proteins (Grb2 and Nck) (1-3). In addition, BLNK binds Btk and is required for activation of the transcription factor NF-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BLNK