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BHMT Antibody (3D6) - BSA Free

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Western Blot: BHMT Antibody (3D6) [NBP1-04264] - The extracts of mouse liver (each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western Blot: BHMT Antibody (3D6) [NBP1-04264] - The extracts of mouse liver (each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
3D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-04264AF350
Alexa Fluor 405
NBP1-04264AF405
Alexa Fluor 488
NBP1-04264AF488
Alexa Fluor 532
NBP1-04264AF532
Alexa Fluor 594
NBP1-04264AF594
Alexa Fluor 647
NBP1-04264AF647
Alexa Fluor 700
NBP1-04264AF700
Alexa Fluor 750
NBP1-04264AF750
Biotin
NBP1-04264B
CoraFluor 1
NBP1-04264CL1
DyLight 350
NBP1-04264UV
DyLight 405
NBP1-04264V
DyLight 488
NBP1-04264G
DyLight 550
NBP1-04264R
DyLight 594
NBP1-04264DL594
DyLight 650
NBP1-04264C
DyLight 680
NBP1-04264FR
DyLight 755
NBP1-04264IR
FITC
NBP1-04264F
HRP
NBP1-04264H
Janelia Fluor 525
NBP1-04264JF525
Janelia Fluor 549
NBP1-04264JF549
Janelia Fluor 585
NBP1-04264JF585
Janelia Fluor 635
NBP1-04264JF635
Janelia Fluor 646
NBP1-04264JF646
Janelia Fluor 669
NBP1-04264JF669
mFluor Violet 450 SE
NBP1-04264MFV450
mFluor Violet 500 SE
NBP1-04264MFV500
mFluor Violet 610 SE
NBP1-04264MFV610
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BHMT Antibody (3D6) - BSA Free Summary

Immunogen
Recombinant human BHMT (1-406aa) purified from E. coli
Specificity
NP_001704
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BHMT
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for BHMT Antibody (3D6) - BSA Free

  • betaine homocysteine methyltransferase
  • betaine-homocysteine methyltransferase
  • betaine--homocysteine S-methyltransferase 1
  • betaine--homocysteine S-methyltransferase
  • BHMT1
  • EC 2.1.1
  • EC 2.1.1.5

Background

Betaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BHMT