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![Western Blot: BHMT Antibody [NBP1-00161] - Rat Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-00161-img0003.jpg "Western Blot: BHMT Antibody [NBP1-00161] - Rat Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Kidney tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-00161-img0005.jpg)
![Western Blot: BHMT Antibody [NBP1-00161] - HEK293 overexpressing BHMT and probed with this antibody (mock transfection in first lane).](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-00161-img0001.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Liver tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-00161-img0004.jpg)
![Western Blot: BHMT Antibody (3E11) [NBP2-02349] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.](http://images.novusbio.com/fullsize/BHMT-Antibody-3E11-Western-Blot-NBP2-02349-img0002.jpg "Western Blot: BHMT Antibody (3E11) [NBP2-02349] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.")
![Immunocytochemistry/Immunofluorescence: BHMT Antibody (3E11) [NBP2-02349] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.](http://images.novusbio.com/fullsize/BHMT-Antibody-3E11-Immunocytochemistry-Immunofluorescence-NBP2-02349-img0007.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody (3E11) [NBP2-02349] - Staining of paraffin-embedded liver tissue using anti-BHMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BHMT-Antibody-3E11-Immunohistochemistry-Paraffin-NBP2-02349-img0006.jpg)
![Flow Cytometry: BHMT Antibody (3E11) [NBP2-02349] - HEK293T cells transfected with either pCMV6-ENTRY BHMT.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BHMT-Antibody-3E11-Flow-Cytometry-NBP2-02349-img0001.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody (3E11) [NBP2-02349] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BHMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BHMT-Antibody-3E11-Immunohistochemistry-Paraffin-NBP2-02349-img0003.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody (3E11) [NBP2-02349] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-BHMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BHMT-Antibody-3E11-Immunohistochemistry-Paraffin-NBP2-02349-img0004.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody (3E11) [NBP2-02349] - Staining of paraffin-embedded Kidney tissue using anti-BHMT mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BHMT-Antibody-3E11-Immunohistochemistry-Paraffin-NBP2-02349-img0005.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-88611] - Staining in human kidney and lymph node tissues using anti-BHMT antibody. Corresponding BHMT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-88611-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-88611] - Staining in human kidney and lymph node tissues using anti-BHMT antibody. Corresponding BHMT RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-88611] - Staining of human cerebral cortex, colon, kidney and testis using Anti-BHMT antibody NBP1-88611 (A) shows similar protein distribution across tissues to independent antibody NBP2-38850 (B).](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-88611-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: BHMT Antibody [NBP1-88611] - Analysis using Anti-BHMT antibody NBP1-88611 (A) shows similar pattern to independent antibody NBP2-38850 (B).](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-88611-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: BHMT Antibody [NBP1-88611] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88611-img0006.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-88611] - Staining of human kidney using Anti-BHMT antibody NBP1-88611.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-88611-img0016.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-88611] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-88611-img0007.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-88611] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-88611-img0008.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-88611] - Staining of human colon using Anti-BHMT antibody.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-88611-img0013.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-88611] - Staining of human cerebral cortex using Anti-BHMT antibody.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-88611-img0014.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-88611] - Staining of human testis using Anti-BHMT antibody.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-88611-img0015.jpg)
![Western Blot: BHMT Antibody [NBP2-38850] - Analysis using Anti-BHMT antibody NBP2-38850 (A) shows similar pattern to independent antibody NBP1-88611 (B).](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP2-38850-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: BHMT Antibody [NBP2-38850] - Analysis using Anti-BHMT antibody NBP2-38850 (A) shows similar pattern to independent antibody NBP1-88611 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP2-38850-img0010.jpg)
![Western Blot: BHMT Antibody [NBP2-38850] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP2-38850-img0002.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP2-38850-img0003.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP2-38850-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining in human kidney and lymph node tissues using anti-BHMT antibody. Corresponding BHMT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP2-38850-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human cerebral cortex, colon, kidney and testis using Anti-BHMT antibody NBP2-38850 (A) shows similar protein distribution across tissues to independent antibody NBP1-88611 (B).](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP2-38850-img0006.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human colon.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP2-38850-img0008.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human testis.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP2-38850-img0009.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP2-38850] - Staining of human kidney using Anti-BHMT antibody NBP2-38850.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP2-38850-img0011.jpg)
![Western Blot: BHMT Antibody [NBP1-32443] - Sample(30 ug whole cell lysate)A:Hep G2 10% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-32443-img0004.jpg "Western Blot: BHMT Antibody [NBP1-32443] - Sample(30 ug whole cell lysate)A:Hep G2 10% SDS PAGE diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: BHMT Antibody [NBP1-32443] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32443-img0002.jpg)
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-32443] - Paraffin-embedded Colon Carcinoma; antibody dilution 1:500.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-32443-img0003.jpg)
![Western Blot: BHMT Antibody [NBP1-32443] - Sample (30 ug of whole cell lysate) A: whole zebrafish 10% SDS PAGE NBP1-32443 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-32443_rabbit-polyclonal-bhmt-antibody-91020242091453.jpg)
![Immunohistochemistry: BHMT Antibody [NBP1-32443] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using BHMT antibody (NBP1-32443) at 1:300 dilution.](http://images.novusbio.com/fullsize/nbp1-32443_rabbit-polyclonal-bhmt-antibody-910202420131236.jpg)
![Western Blot: BHMT Antibody [NBP1-32443] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BHMT antibody (NBP1-32443) diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp1-32443_rabbit-polyclonal-bhmt-antibody-91020242041264.jpg)
![Western Blot: BHMT Antibody [NBP1-55288] - Lanes: Lane1: 20 ug rat liver lysate Primary Antibody Dilution: 1:2000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:15000 Gene Name: BHMT Submitted by: Anonymous](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-55288-img0007.jpg "Western Blot: BHMT Antibody [NBP1-55288] - Lanes: Lane1: 20 ug rat liver lysate Primary Antibody Dilution: 1:2000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:15000 Gene Name: BHMT Submitted by: Anonymous")
![Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-55288] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/BHMT-Antibody-Immunohistochemistry-Paraffin-NBP1-55288-img0003.jpg)
![Western Blot: BHMT Antibody [NBP1-55288] - Analysis of 293T cell lysate. Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-55288-img0004.jpg)
![Western Blot: BHMT Antibody [NBP1-55288] - Lanes: Lane1 : 20 ug rat liver lysate Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1 : 15000 Gene name: BHMT Submitted by: Anonymous.](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-55288-img0005.jpg)
![Western Blot: BHMT Antibody [NBP1-55288] - Reccomended Titration: 2.5 ug/ml Positive Control: Human Fetal Liver cell lysate](http://images.novusbio.com/fullsize/BHMT-Antibody-Western-Blot-NBP1-55288-img0006.jpg)
![Western Blot: BHMT Antibody (9E1-R) [NBP3-32082] - Western blot analysis of BHMT on different lysates with Mouse anti-BHMT antibody (NBP3-32082) at 1/1,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32082) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32082_mouse-bhmt-mab-9e1-r-278202415295557.jpg "Western Blot: BHMT Antibody (9E1-R) [NBP3-32082] - Western blot analysis of BHMT on different lysates with Mouse anti-BHMT antibody (NBP3-32082) at 1/1,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32082) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: BHMT Antibody (9E1-R) [NBP3-32082] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-BHMT antibody (NBP3-32082) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32082) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32082_mouse-bhmt-mab-9e1-r-298202414262613.jpg)
![Western Blot: BHMT Antibody (3D6) [NBP1-04264] - The extracts of mouse liver (each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/BHMT-Antibody-3D6-Western-Blot-NBP1-04264-img0001.jpg "Western Blot: BHMT Antibody (3D6) [NBP1-04264] - The extracts of mouse liver (each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Western Blot: BHMT Antibody (7O7D7) [NBP3-16570] - Western blot analysis of extracts of various cell lines, using BHMT Rabbit mAb (NBP3-16570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/BHMT-Antibody-7O7D7-Western-Blot-NBP3-16570-img0001.jpg "Western Blot: BHMT Antibody (7O7D7) [NBP3-16570] - Western blot analysis of extracts of various cell lines, using BHMT Rabbit mAb (NBP3-16570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: BHMT Antibody (SR1760) [NBP3-22177] - Western blot analysis using NBP3-22177 in Human fetal kidney lysate.](http://images.novusbio.com/fullsize/nbp3-22177_rabbit-bhmt-mab-sr1760-5720231649422.jpg "Western Blot: BHMT Antibody (SR1760) [NBP3-22177] - Western blot analysis using NBP3-22177 in Human fetal kidney lysate.")
![Western Blot: BHMT Overexpression Lysate (Adult Normal) [NBL1-07976] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BHMT.](http://images.novusbio.com/fullsize/BHMT-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07976-img0002.jpg "Western Blot: BHMT Overexpression Lysate (Adult Normal) [NBL1-07976] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BHMT.")
![Recombinant Human BHMT Protein [NBC1-18523]](http://images.novusbio.com/fullsize/Recombinant-Human-BHMT-Protein-NBC1-18523-img0001.jpg "Recombinant Human BHMT Protein [NBC1-18523]")