![Western Blot: beta Tubulin Antibody (S11B) [NBP2-81064] - Western Blot using anti-Beta-Tubulin antibody (S11B) [NBP2-81064]. HeLa (A), A431 (B), HEK293 (C) and MCF-7 (D) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of S11B [NBP2-81064] at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-Tubulin is ~54kDa. NBP2-81064 successfully detected human Beta-Tubulin in HeLa, A431, HEK293 and MCF-7 cell lysate samples.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-S11B-Western-Blot-NBP2-81064-img0004.jpg "Western Blot: beta Tubulin Antibody (S11B) [NBP2-81064] - Western Blot using anti-Beta-Tubulin antibody (S11B) [NBP2-81064]. HeLa (A), A431 (B), HEK293 (C) and MCF-7 (D) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of S11B [NBP2-81064] at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-Tubulin is ~54kDa. NBP2-81064 successfully detected human Beta-Tubulin in HeLa, A431, HEK293 and MCF-7 cell lysate samples.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | S11B |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Human MBP (microtubule-binding protein). |
| Specificity | Binds specifically to human beta Tubulin (no crossreactivity with alpha-tubulin). |
| Isotype | IgG Lambda |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TUBB |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to beta-tubulin. Tubulin is the major constituent of microtubules. The beta-chain has an exchangeable GTP-binding site. |
| Storage | Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C. |
| Buffer | PBS |
| Preservative | 0.02% Proclin 300 |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for beta Tubulin Antibody (NBP2-81064)Find related products by research area.
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| Gene Symbol | TUBB |
![Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (S11B) [NBP2-81064] - Immunofluoresence staining of fixed HeLa cells with anti-Beta-tubulin antibody (S11B) [NBP2-81064]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of S11B [NBP2-81064] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81064, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-S11B-Immunocytochemistry-Immunofluorescence-NBP2-81064-img0003.jpg "Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (S11B) [NBP2-81064] - Immunofluoresence staining of fixed HeLa cells with anti-Beta-tubulin antibody (S11B) [NBP2-81064]. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of S11B [NBP2-81064] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81064, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.")
![Immunohistochemistry-Paraffin: beta Tubulin Antibody (S11B) [NBP2-81064] - Immunohistochemical staining of human cerebral cortex tissue using [NBP2-81064]. Anti-Beta Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of S11B [NBP2-81064]. Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-81064 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of neuronal cell bodies and their processes may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-S11B-Immunohistochemistry-Paraffin-NBP2-81064-img0002.jpg "Immunohistochemistry-Paraffin: beta Tubulin Antibody (S11B) [NBP2-81064] - Immunohistochemical staining of human cerebral cortex tissue using [NBP2-81064]. Anti-Beta Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of S11B [NBP2-81064]. Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-81064 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of neuronal cell bodies and their processes may be observed. Recommended concentration, 2-4 ug/ml.")
![Flow Cytometry: beta Tubulin Antibody (S11B) [NBP2-81064] - Flow-cytometry using the anti-Beta Tubulin (S11B) [NBP2-81064]. MCF-7 (A) and HeLa (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of S11B (NBP2-81064, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/beta-Tubulin-Antibody-S11B-Flow-Cytometry-NBP2-81064-img0001.jpg "Flow Cytometry: beta Tubulin Antibody (S11B) [NBP2-81064] - Flow-cytometry using the anti-Beta Tubulin (S11B) [NBP2-81064]. MCF-7 (A) and HeLa (B) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of S11B (NBP2-81064, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
