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Rat beta-Galactosidase-1/GLB1 ELISA Kit (Colorimetric)

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ELISA: Rat beta-Galactosidase-1/GLB1 ELISA Kit (Colorimetric) [NBP2-82182] - Samples were spiked with high concentrations of Rat beta-Galactosidase-1/GLB1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat beta-Galactosidase-1/GLB1 ELISA Kit (Colorimetric) [NBP2-82182] - Samples were spiked with high concentrations of Rat beta-Galactosidase-1/GLB1 and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Rat beta-Galactosidase-1/GLB1 ELISA Kit (Colorimetric) [NBP2-82182] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

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Rat beta-Galactosidase-1/GLB1 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat beta-Galactosidase-1/GLB1 in samples. No Significant cross-reactivity or interference between Rat beta-Galactosidase-1/GLB1 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 6%
Intra-Assay
CV% < 6%
Spike Recovery
average = 94% for serum; average = 91% for EDTA plasma; average = 93% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GLB1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Rat beta-Galactosidase-1/GLB1 ELISA Kit (Colorimetric)

  • Acid beta-galactosidase
  • beta-galactosidase
  • betaGalactosidase1
  • beta-Galactosidase-1
  • EBP
  • EC 3.2.1.23
  • elastin receptor 1 (67kD)
  • Elastin receptor 1
  • elastin receptor 1, 67kDa
  • ELNR1
  • galactosidase, beta 1
  • GLB1
  • Lactase
  • MPS4B

Background

The GLB1 gene encodes beta-galactosidase-1 (EC 3.2.1.23), a lysosomal hydrolase that cleaves the terminal beta-galactose from ganglioside substrates and other glycoconjugates (Yoshida et al., 1991 [PubMed 1907800]). Beta-galactosidase also occurs in a complex with neuraminidase (NEU1; MIM 608272) and protective protein/cathepsin A (PPCA; MIM 256540), which is a component of certain cell surface receptors (Hinek, 1996 [PubMed 8922281]). See also galactosylceramidase (GALC; MIM 606890) (EC 3.2.1.46), a genetically distinct beta-galactosidase that is involved in the catabolism of other lipid compounds.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Rat beta-Galactosidase-1/GLB1 ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol GLB1