![Western Blot: beta-Actin Antibody (CL3868) [NBP2-76481] - Lane 1: Molecular weight marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line HeLa. Lane 3: Human cell line HEK 293. Lane 4: Human cell line A-431. Lane 5: Human cell line HepG2. Lane 6: Mouse cell line NIH-3T3. Lane 7: Rat cell line NBT-II.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-CL3868-Western-Blot-NBP2-76481-img0001.jpg "Western Blot: beta-Actin Antibody (CL3868) [NBP2-76481] - Lane 1: Molecular weight marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line HeLa. Lane 3: Human cell line HEK 293. Lane 4: Human cell line A-431. Lane 5: Human cell line HepG2. Lane 6: Mouse cell line NIH-3T3. Lane 7: Rat cell line NBT-II.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | CL3868 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | This beta-Actin Antibody (CL3868) was developed against a recombinant protein corresponding to amino acids: PKANREKMTQIMFETFNTPAMYVAIQ |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ACTB |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. |
| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.2, containing 40% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ACTB |
![Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481] - Staining of human tonsil shows strong positivity in lymphoid cells.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-CL3868-Immunohistochemistry-Paraffin-NBP2-76481-img0004.jpg "Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481] - Staining of human tonsil shows strong positivity in lymphoid cells.")
![Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481] - Staining of human prostate shows strong cytoplasmic immunoreactivity in smooth muscle and weak positivity in glandular cells.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-CL3868-Immunohistochemistry-Paraffin-NBP2-76481-img0002.jpg "Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481] - Staining of human prostate shows strong cytoplasmic immunoreactivity in smooth muscle and weak positivity in glandular cells.")
![Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481] - Staining of human fallopian tube shows immunoreactivity in smooth muscle cells of blood vessels and connective tissue.](http://images.novusbio.com/fullsize/beta-Actin-Antibody-CL3868-Immunohistochemistry-Paraffin-NBP2-76481-img0003.jpg "Immunohistochemistry-Paraffin: beta-Actin Antibody (CL3868) [NBP2-76481] - Staining of human fallopian tube shows immunoreactivity in smooth muscle cells of blood vessels and connective tissue.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")