![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Lane 1: HEK cells. Lane 2: HEK cells with siRNA against beta 2-Microglobulin antibody. Lane 3: HEK cells stimulated for increased beta 2-Microglobulin expression. Image from verified customer review.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0015.jpg "Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Lane 1: HEK cells. Lane 2: HEK cells with siRNA against beta 2-Microglobulin antibody. Lane 3: HEK cells stimulated for increased beta 2-Microglobulin expression. Image from verified customer review.")
| Reactivity | Hu, Mu, Rt, Pm, Pm, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Full length human beta 2-Microglobulin Recombinant protein. |
| Localization | Secreted |
| Predicted Species | Rhesus Macaque (92%), Chimpanzee (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | B2M |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS, 1% BSA, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Images | Ratings | Applications | Species | Date | Details | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Lamberto Torralba |
WB | Human | 05/09/2022 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for beta 2-Microglobulin Antibody (NBP2-15566)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody [NBP2-15566] - beta 2-Microglobulin antibody detects beta 2 Microglobulin protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta 2-Microglobulin stained by beta 2-Microglobulin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15566-img0013.jpg "Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody [NBP2-15566] - beta 2-Microglobulin antibody detects beta 2 Microglobulin protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta 2-Microglobulin stained by beta 2-Microglobulin antibody diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse spleen. B2M stained by Beta 2 Microglobulin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15566-img0016.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse spleen. B2M stained by Beta 2 Microglobulin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0008.jpg "Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:500.")
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse plasma (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:1500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0012.jpg "Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse plasma (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:1500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2-Microglobulin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Western-Blot-NBP2-15566-img0014.jpg "Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2-Microglobulin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Cal27 xenograft. beta 2 Microglobulin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15566-img0005.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Cal27 xenograft. beta 2 Microglobulin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Human endometrial cancer. beta 2 Microglobulin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunohistochemistry-Paraffin-NBP2-15566-img0007.jpg "Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Human endometrial cancer. beta 2 Microglobulin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunoprecipitation: beta 2-Microglobulin Antibody [NBP2-15566] - HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of B2M protein by 4 ug beta 2 Microglobulin antibody 15 % SDS-PAGE The mmunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-Immunoprecipitation-NBP2-15566-img0010.jpg "Immunoprecipitation: beta 2-Microglobulin Antibody [NBP2-15566] - HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of B2M protein by 4 ug beta 2 Microglobulin antibody 15 % SDS-PAGE The mmunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15566_rabbit-polyclonal-beta-2-microglobulin-antibody-western-blot-16122024101912..png "Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![SDS-PAGE TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNF-alpha_210-TA_256.jpg)
![Bioactivity TNF-alpha [Unconjugated]](https://images.novusbio.com/images2/TNFalpha_210TA_1658.jpg)
![SEC-MALS TNF-alpha [Unconjugated]](https://images.novusbio.com/images/210-ta_recombinant-human-tnf-alpha-protein-sec-mals-35202312244..jpg)
![N/A C-Reactive Protein/CRP [Biotin]](https://images.novusbio.com/images2/DATA_CReactive_Protein_DY1707_ELISA_1701.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/DATA_IL6_M6000_ELISA_936.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images2/IL-6_M6000_ELISA_415.jpg)
![N/A IL-6 [HRP]](https://images.novusbio.com/images/m6000b_mouse-il-6-quantikine-elisa-kit-44202415433118.jpg)
![Bioactivity GM-CSF [Unconjugated]](https://images.novusbio.com/images/protein/7954-gmcf_recombinant-human-gm-csf-cho-expressed-protein-cf-bioactivity-272020121514.jpg)
![N/A Cystatin C [HRP]](https://images.novusbio.com/images2/Cystatin_C_MSCTC0_ELISA_321.jpg)
![N/A Cystatin C [HRP]](https://images.novusbio.com/images2/Cystatin_C_MSCTC0_ELISA_322.jpg)
![N/A Cystatin C [HRP]](https://images.novusbio.com/images2/DATA_Cystatin_C_MSCTC0_ELISA_1030.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
