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beta 2-Microglobulin Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining in human lymph node and skeletal muscle tissues using anti-B2M antibody. Corresponding B2M RNA-seq data ...read more
Orthogonal Strategies: Western Blot: beta 2-Microglobulin Antibody [NBP1-87482] - Analysis in human cell lines HeLa and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: ...read more
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human cell line U-251 MG shows localization to plasma membrane, cytosol and the Golgi apparatus. Antibody staining is ...read more
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neurons as expected.
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP1-87482] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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beta 2-Microglobulin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
B2M
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
beta 2-Microglobulin Protein (NBP1-87482PEP)
Publications
Read Publications using
NBP1-87482 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for beta 2-Microglobulin Antibody

  • B2M
  • beta 2Microglobulin
  • beta 2-Microglobulin
  • beta chain of MHC class I molecules
  • beta-2-microglobin
  • beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta 2-Microglobulin Antibody (NBP1-87482)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Immunohistochemistry-Paraffin.


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Immunohistochemistry-Paraffin
(1)
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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol B2M