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beta 2-Microglobulin Antibody (B2M/1118) - Azide and BSA Free

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Western Blot: beta 2-Microglobulin Antibody (B2M/1118) - Azide and BSA Free [NBP2-47702] - Western Blot Analysis of THP-1 and Raji Cell lysate using beta 2-Microglobulin Antibody (B2M/1118).
Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody (B2M/1118) - Azide and BSA Free [NBP2-47702] - Immunofluorescent staining of HeLa cells. beta 2-Microglobulin Mouse Monoclonal Antibody (B2M/1118) ...read more
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) - Azide and BSA Free [NBP2-47702] - Human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/1118)
Flow Cytometry: beta 2-Microglobulin Antibody (B2M/1118) - Azide and BSA Free [NBP2-47702] - Flow Cytometric Analysis of HeLa cells using beta 2-Microglobulin Mouse Monoclonal Antibody (B2M/1118); followed by goat ...read more
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) - Azide and BSA Free [NBP2-47702] - Human Cervical Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/1118)
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (B2M/1118) - Azide and BSA Free [NBP2-47702] - Human Endometrial Carcinoma stained with Beta-2-Microglobulin Monoclonal Antibody (B2M/1118)

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
Clone
B2M/1118
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

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beta 2-Microglobulin Antibody (B2M/1118) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44467).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Full length recombinant human beta 2-Microglobulin protein (Uniprot: P61769)
Localization
Cell Surface and Extracellular (Secreted)
Marker
Renal Failure & Tumor Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
B2M
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Cell Surface) 1:10-1:1000
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Non-Human primates.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for beta 2-Microglobulin Antibody (B2M/1118) - Azide and BSA Free

  • B2M
  • beta 2Microglobulin
  • beta 2-Microglobulin
  • beta chain of MHC class I molecules
  • beta-2-microglobin
  • beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol B2M