![Western Blot: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467] - Western Blot Analysis of THP-1 and Raji Cell lysate using beta 2-Microglobulin Antibody (B2M/1118).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-1118-Western-Blot-NBP2-44467-img0009.jpg "Western Blot: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467] - Western Blot Analysis of THP-1 and Raji Cell lysate using beta 2-Microglobulin Antibody (B2M/1118).")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, ICC/IF |
| Clone | B2M/1118 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47702) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Full length recombinant human beta 2-Microglobulin protein (Uniprot: P61769) |
| Localization | Cell Surface and Extracellular (Secreted) |
| Marker | Renal Failure & Tumor Marker |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | B2M |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 12 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467] - Immunofluorescent staining of HeLa cells. beta 2-Microglobulin Mouse Monoclonal Antibody (B2M/1118) followed by goat anti-Mouse IgG-CF488 (Green).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-B2M-1118-Immunocytochemistry-Immunofluorescence-NBP2-44467-img0007.jpg "Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M/1118) [NBP2-44467] - Immunofluorescent staining of HeLa cells. beta 2-Microglobulin Mouse Monoclonal Antibody (B2M/1118) followed by goat anti-Mouse IgG-CF488 (Green).")
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