Bcl-2 Antibody (OTI9D3) - Azide and BSA Free

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Western Blot: Bcl-2 Antibody (OTI9D3) - Azide and BSA Free [NBP2-70245] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL-2.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI9D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

Bcl-2 Antibody (OTI9D3) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human BCL2 (NP_000624) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
BCL2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
26.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Bcl-2 Antibody (OTI9D3) - Azide and BSA Free

  • apoptosis regulator Bcl-2
  • B-cell CLL/lymphoma 2
  • Bcl2
  • Bcl-2

Background

The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol BCL2