In the presence of Goat F(ab')2 Anti-mouse IgM, Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 8876-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, ...read more
Detects mouse BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human (rh) BAFF is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFSF13B
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
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Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for BAFF/BLyS/TNFSF13B Antibody [Unconjugated]
ApoL related ligand TALL-1
B lymphocyte stimulator
BAFF
BAFFB-cell activating factor
B-cell-activating factor
BLyS
BLYSB-lymphocyte stimulator
CD257 antigen
CD257
Dendritic cell-derived TNF-like molecule
DTL
TALL1
TALL-1delta BAFF
TALL1Delta4 BAFF
THANK
TNF- and APOL-related leukocyte expressed ligand 1
TNF and ApoL-related leukocyte expressed ligand 1
TNF homolog that activates apoptosis
TNFSF13B
TNFSF20
tumor necrosis factor (ligand) superfamily, member 13b
tumor necrosis factor (ligand) superfamily, member 20
tumor necrosis factor ligand superfamily member 13B
tumor necrosis factor superfamily, member 13b
tumor necrosis factor-like protein ZTNF4
ZTNF4
Background
BAFF (also known as TALL-1, BLyS, THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 aa protein consisting of a 248 amino acid (aa) extracellular domain, a 21 aa transmembrane region and a 45 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Mouse BAFF may be shed from the cell surface by proteolytic cleavage between R126 and Ala 127 to yield a soluble form of the protein detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6-8). All three receptors are type III transmembrane proteins that are expressed in B cells. BAFF and APRIL can form active heteromers that bind TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is upregulated by IFN-alpha , IFN-beta , LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).
Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145.
Liu, Y. et al. (2002) Cell 108:383.
Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313.
Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
Thompson, J.S. et al. (2001) Science 293:2108.
Ng, L. G. et al. (2004) J. Immunol. 173:807.
Roschke, V. et al. (2002) J. Immunol. 169:4314.
Batten, M. et al. (2000) J. Exp. Med. 192:1453.
Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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