Home » BAFF/BLyS/TNFSF13B » BAFF/BLyS/TNFSF13B Antibodies » BAFF/BLyS/TNFSF13B Antibody [Unconjugated]
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BAFF/BLyS/TNFSF13B Antibody [Unconjugated]

 (15 Publications)
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In the presence of Goat F(ab')2 Anti-mouse IgM (1 µg/mL), Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124). The ND50 is typically3‑12 ng/mL.
In the presence of Goat F(ab')2 Anti-mouse IgM (1 µg/mL), Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Under these ...read more
BAFF/BLyS/TNFSF13B was detected in formalin fixed paraffin-embedded sections of human spleen using 15 µg/mL Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124) overnight ...read more
Role of p65 activation in BLyS up-regulation. (A) HIF-1 alpha and p65 protein levels in MDA-MB-435 in hypoxic conditions for different time points by Western Blotting. (B) CAPE(50 μM)and PX 12 (10 μM) were used to ...read more
Expressions of BLyS, TACI, BCMA and BAFF-R in human breast cancer cell lines. (A) BLyS and its three receptors in human breast cancer cell lines MDA-MB-435, MDA-MB-231, MDA-MB-468 and B cell line Ramos by ...read more
Expressions of BLyS, TACI, BCMA and BAFF-R in human breast cancer cell lines. (A) BLyS and its three receptors in human breast cancer cell lines MDA-MB-435, MDA-MB-231, MDA-MB-468 and B cell line Ramos by ...read more
Role of p65 activation in BLyS up-regulation. (A) HIF-1 alpha and p65 protein levels in MDA-MB-435 in hypoxic conditions for different time points by Western Blotting. (B) CAPE(50 μM)and PX 12 (10 μM) were used to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF124
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF124-SP
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BAFF/BLyS/TNFSF13B Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human BAFF/BLyS/TNFSF13B
Ala134-Leu285
Accession # Q9Y275
Specificity
Detects human BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant murine BAFF and less than 1% cross‑reactivity with recombinant human (rh) APRIL is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFSF13B
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.60 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 3-12 ng/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF124 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BAFF/BLyS/TNFSF13B Antibody [Unconjugated]

  • ApoL related ligand TALL-1
  • B lymphocyte stimulator
  • BAFF
  • BAFFB-cell activating factor
  • B-cell-activating factor
  • BLyS
  • BLYSB-lymphocyte stimulator
  • CD257 antigen
  • CD257
  • Dendritic cell-derived TNF-like molecule
  • DTL
  • TALL1
  • TALL-1delta BAFF
  • TALL1Delta4 BAFF
  • THANK
  • TNF- and APOL-related leukocyte expressed ligand 1
  • TNF and ApoL-related leukocyte expressed ligand 1
  • TNF homolog that activates apoptosis
  • TNFSF13B
  • TNFSF20
  • tumor necrosis factor (ligand) superfamily, member 13b
  • tumor necrosis factor (ligand) superfamily, member 20
  • tumor necrosis factor ligand superfamily member 13B
  • tumor necrosis factor superfamily, member 13b
  • tumor necrosis factor-like protein ZTNF4
  • ZTNF4

Background

BAFF (also known as TALL-1, BLyS, and THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Human BAFF is a 285 amino acid (aa) protein consisting of a 218 aa extracellular domain, a 21 aa transmembrane region and a 46 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Human BAFF may be shed from the cell surface by proteolytic cleavage between R133 and Ala134 to yield a soluble form of the protein that is detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6‑8). All three receptors are type III transmembrane proteins that are expressd in B cells. BAFF and APRIL can form active heteromers that bind to TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha , IFN-beta , LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

  1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
  3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145.
  4. Liu, Y. et al. (2002) Cell 108:383.
  5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313.
  6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  7. Thompson, J.S. et al. (2001) Science 293:2108.
  8. Ng, L.G. et al. (2004) J. Immunol. 173:807.
  9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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BAFF/BLyS/TNFSF13B
Antibody [Unconjugate ...
AF124
BAFF/BLyS/TNFSF13B Antibody [Unconjugated]
Species: Hu
Applications: WB, IHC, B/N

Publications for BAFF/BLyS/TNFSF13B Antibody (AF124)(15)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 5 applications: ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
ICC
(3)
IHC
(2)
IHC-P
(2)
Neutralization
(5)
Western Blot
(1)
All Applications
Filter By Species
Human
(11)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF124 Applications Species
Liu, Y;Ye, SY;He, S;Chi, DM;Wang, XZ;Wen, YF;Ma, D;Nie, RC;Xiang, P;Zhou, Y;Ruan, ZH;Peng, RJ;Luo, CL;Wei, PP;Lin, GW;Zheng, J;Cui, Q;Cai, MY;Yun, JP;Dong, J;Mai, HQ;Xia, X;Bei, JX; Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma Nature communications 2024-09-04 [PMID: 39231979] (Neutralization, Human) Neutralization Human
Asgar Ansari, Shilpa Sachan, Bimal Prasad Jit, Ashok Sharma, Poonam Coshic, Alessandro Sette et al. An efficient immunoassay for the B cell help function of SARS-CoV-2-specific memory CD4+ T cells Cell Reports Methods 2022-06-20 [PMID: 35571764]
H Talbot, S Saada, E Barthout, PF Gallet, N Gachard, J Abraham, A Jaccard, D Troutaud, F Lalloué, T Naves, AL Fauchais, MO Jauberteau BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells Sci Rep, 2020-07-28;10(1):12572. 2020-07-28 [PMID: 32724091] (Neutralization, Human) Neutralization Human
Mario Rothbauer, Gregor Höll, Christoph Eilenberger, Sebastian R. A. Kratz, Bilal Farooq, Patrick Schuller et al. Monitoring tissue-level remodelling during inflammatory arthritis using a three-dimensional synovium-on-a-chip with non-invasive light scattering biosensing Lab on a Chip 2020-03-29 [PMID: 32219235]
Y Cao, G Lu, X Chen, X Chen, N Guo, W Li BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF?&amp;kappaB signaling pathway in glomerular mesangial cells Mol Med Rep, 2019-12-06;21(2):795-805. 2019-12-06 [PMID: 31974601] (IHC, Human) IHC Human
M Imbrechts, K De Samblan, K Fierens, E Brisse, J Vandenhaut, T Mitera, C Libert, I Smets, A Goris, C Wouters, P Matthys IFN-? stimulates CpG-induced IL-10 production in B cells via p38 and JNK signalling pathways Eur. J. Immunol., 2018-08-01;0(0):. 2018-08-01 [PMID: 30004580] (Neutralization, Human) Neutralization Human
O Berggren, N Hagberg, A Alexsson, G Weber, L Rönnblom, ML Eloranta Plasmacytoid dendritic cells and RNA-containing immune complexes drive expansion of peripheral B cell subsets with an SLE-like phenotype PLoS ONE, 2017-08-28;12(8):e0183946. 2017-08-28 [PMID: 28846748] (Neutralization, Human) Neutralization Human
N Zheng, J Fan, B Wang, D Wang, P Feng, Q Yang, X Yu Expression profile of BAFF in peripheral blood from patients of IgA nephropathy: Correlation with clinical features and Streptococcus�pyogenes infection Mol Med Rep, 2017-02-10;0(0):. 2/10/2017 [PMID: 28260100]
Hamada T, Samukawa T, Kumamoto T, Hatanaka K, Tsukuya G, Yamamoto M, Machida K, Watanabe M, Mizuno K, Higashimoto I, Inoue Y, Inoue H Serum B cell-activating factor (BAFF) level in connective tissue disease associated interstitial lung disease. BMC Pulm Med, 2015-09-30;15(0):110. 2015-09-30 [PMID: 26424433] (IHC-P, Human) IHC-P Human
Abe T, AlSarhan M, Benakanakere M, Maekawa T, Kinane D, Cancro M, Korostoff J, Hajishengallis G The B Cell-Stimulatory Cytokines BLyS and APRIL Are Elevated in Human Periodontitis and Are Required for B Cell-Dependent Bone Loss in Experimental Murine Periodontitis. J Immunol, 2015-07-06;195(4):1427-35. 2015-07-06 [PMID: 26150532] (IHC, Human) IHC Human
Show All 15 Publications.

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Bioinformatics

Gene Symbol TNFSF13B
Uniprot