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Bad Antibody (612312) [Unconjugated]

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Western blot shows lysates of A431 human epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Bad Monoclonal Antibody (Catalog # MAB6405) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bad at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western blot shows lysates of A431 human epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and NRK rat normal kidney cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse ...read more
Bad was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human Bad Monoclonal Antibody (Catalog # MAB6405) at 25 µg/mL for 3 hours at room temperature. Cells were stained using ...read more
Bad was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat Bad Monoclonal Antibody (Catalog # MAB6405) at 15 µg/mL overnight at 4 °C. Before incubation with the ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bad knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bad ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF, KO
Clone
612312
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6405-SP
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Bad Antibody (612312) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Bad
Met1-Gln168
Accession # Q92934
Specificity
Detects human Bad in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) Bad (aa 1-130), rmBcl-2 alpha , recombinant human (rh) Bcl-2, or rhBCL2L12 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BAD
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Knockout Validated
  • Western Blot 0.5 ug/mL
Publications
Read Publication using
MAB6405 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bad Antibody (612312) [Unconjugated]

  • Bad
  • BBC2
  • BBC6
  • bcl2 antagonist of cell death
  • BCL2-antagonist of cell death protein
  • BCL2-associated agonist of cell death
  • Bcl-2-binding component 6
  • BCL2-binding component 6
  • BCL2-binding protein
  • BCL2L8
  • Bcl2-L-8
  • BCL2L8bcl2-L-8
  • Bcl-2-like protein 8
  • BCL-X/BCL-2 binding protein
  • Bcl-XL/Bcl-2-associated death promoter

Background

Bcl-2 antagonist of cell death (Bad) is a 23 kDa cytoplasmic protein in the Bcl-2 family. It functions as a pro-apoptotic molecule by dimerizing with and inhibiting the anti-apoptotic proteins Bcl-2 and Bcl-xL. Prosurvival signals trigger the phosphorylation of Bad on Ser115, disrupting its interaction with Bcl-2 and Bcl-xL and resulting in protection from apoptosis. Human Bad shares 75% aa sequence identity with mouse and rat Bad.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bad Antibody
(612312) [Unconjugate
MAB6405
Bad Antibody (612312) [Unconjugated]
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Publications for Bad Antibody (MAB6405)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol BAD
Uniprot