| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6405-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF819-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Mouse Adsorbed | NB100-56078SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB850-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7764-SP | |||
![Western Blot: Bad Antibody [NB100-56080] - Analysis of Bad in Daoy whole cell lysate using anti-Bad antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Bad-Antibody-Western-Blot-NB100-56080-img0002.jpg "Western Blot: Bad Antibody [NB100-56080] - Analysis of Bad in Daoy whole cell lysate using anti-Bad antibody. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Bad Antibody [NB100-56080] - Immunofluorescence microscopy of BAD using NB100-56080 at 1:2000. Du145 human prostate carcinoma cells were cultured without (A) or with (B) and (C) 1 uM of the triphosphatase inhibitor thapsigargin (THG) for 12 hr. A and B, staining with BAD antibody, followed by a FITC-conjugated secondary antibody. C, staining with a mitochondrial marker (antibody to mitochondrial Hsp60), followed by a rhodamine-conjugated secondary antibody. THG induces Ca2+ release from internal stores which can promote apoptosis. BAD staining was located diffusely throughout the cytoplasm of untreated cells (A), and localized to the mitochondria in treated cells (B).](http://images.novusbio.com/fullsize/Bad-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56080-img0003.jpg)
![Immunohistochemistry-Paraffin: Bad Antibody [NB100-56080] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using 1:2000 conc. of Bad antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of Bad was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Bad-Antibody-Immunohistochemistry-Paraffin-NB100-56080-img0004.jpg)
![Immunohistochemistry-Paraffin: Bad Antibody [NB100-56078] - Analysis of a formalin fixed paraffin-embedded (FFPE) human colon cancer using 1:200 conc. of Bad antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIAR) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Strong apical cytoplasm staining was observed in the cancer cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Bad-Antibody-Immunohistochemistry-Paraffin-NB100-56078-img0001.jpg "Immunohistochemistry-Paraffin: Bad Antibody [NB100-56078] - Analysis of a formalin fixed paraffin-embedded (FFPE) human colon cancer using 1:200 conc. of Bad antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIAR) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Strong apical cytoplasm staining was observed in the cancer cells. Staining was performed by Histowiz.")
![Immunohistochemistry-Paraffin: Bad Antibody [NB100-56079] - Analysis of a formalin fixed paraffin-embedded (FFPE) mouse epididymus tissue using 1:50 dilution of Bad antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIAR) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Bad-Antibody-Immunohistochemistry-Paraffin-NB100-56079-img0001.jpg "Immunohistochemistry-Paraffin: Bad Antibody [NB100-56079] - Analysis of a formalin fixed paraffin-embedded (FFPE) mouse epididymus tissue using 1:50 dilution of Bad antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIAR) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz.")
![Western Blot: Bad [p Ser112] Antibody [NB100-81807] - Analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112)](http://images.novusbio.com/fullsize/Bad-[p-Ser112]-Antibody-Western-Blot-NB100-81807-img0001.jpg "Western Blot: Bad [p Ser112] Antibody [NB100-81807] - Analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112)")
![Immunohistochemistry-Paraffin: Bad [p Ser112] Antibody [NB100-81807] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NB100-81807 (left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/Bad-[p-Ser112]-Antibody-Immunohistochemistry-NB100-81807-img0002.jpg)
![Western Blot: Bad Antibody (1I10A2) [NBP3-15801] - Western blot analysis of extracts of various cell lines, using Bad antibody (NBP3-15801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Bad-Antibody-1I10A2-Western-Blot-NBP3-15801-img0002.jpg "Western Blot: Bad Antibody (1I10A2) [NBP3-15801] - Western blot analysis of extracts of various cell lines, using Bad antibody (NBP3-15801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry-Paraffin: Bad Antibody (1I10A2) [NBP3-15801] - Immunohistochemistry of paraffin-embedded human placenta using Bad antibody (NBP3-15801) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Bad-Antibody-1I10A2-Immunohistochemistry-Paraffin-NBP3-15801-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Bad Antibody [NBP1-88698] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize/Bad-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88698-img0006.jpg "Immunocytochemistry/Immunofluorescence: Bad Antibody [NBP1-88698] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.")
![Immunohistochemistry-Paraffin: Bad Antibody [NBP1-88698] - Staining of human kidney shows strong cytoplasmic positivity in cells of tubules.](http://images.novusbio.com/fullsize/Bad-Antibody-Immunohistochemistry-Paraffin-NBP1-88698-img0002.jpg)