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B7-2/CD86 Antibody [Unconjugated]

 (11 Publications)
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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human B7-2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-141-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for B7-2/CD86 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human B7-2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-141-NA) followed ...read more
B7-2/CD86 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human B7-2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-141-NA) at 15 µg/mL overnight at 4 °C. ...read more
B7-2/CD86 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human B7-2/CD86 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-141-NA) at 3 µg/mL for 1 hour at room ...read more
Recombinant Human B7-2/CD86 Fc Chimera (Catalog # 141-B2) co-stimulates IL-2 secretion in the Jurkat human acute T cell leukemia cell line in the presence of PHA in a dose-dependent manner (orange line), as measured by ...read more
Genetic Strategies: Western blot shows lysates of Ramos human Burkitt's lymphoma parental cell line and B7-2/CD86 knockout Ramos cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

    

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF141
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF-141-SP
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B7-2/CD86 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7‑2/CD86
Ala23-His244
Accession # P42081
Specificity
Detects human B7‑2/CD86 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant mouse B7-2 and recombinant rat B7-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD86
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Neutralization 0.25-1.25 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
AF-141-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-2/CD86 Antibody [Unconjugated]

  • Activation B7-2 antigen
  • B70
  • B7-2 antigen
  • B72
  • B7-2
  • B-lymphocyte activation antigen B7-2
  • BU63
  • CD28 antigen ligand 2
  • CD28LG2B7-2 antigen)
  • CD86 antigen
  • CD86 molecule
  • CD86
  • CTLA-4 counter-receptor B7.2
  • FUN-1
  • LAB72
  • MGC34413
  • T-lymphocyte activation antigen CD86

Background

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20‑100 fold higher affinity than CD28 and is involved in the down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma . B7-1 and B7-2 are both members of the immunoglobulin superfamily. Human B7-2 is a 329 amino acid (aa) protein containing a putative 23 aa signal peptide, a 224 aa extracellular domain, a 21 aa transmembrane domain, and a 61 aa cytoplasmic domain. Human B7-2 and B7-1 share 26% amino acid identity. Human and mouse B7-2 share 50% amino acid identity. However, it has been observed that both human and mouse B7‑1 and
B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

  1. Azuma, M. et al. (1993) Nature 366:76.
  2. Freeman, G.J. et al. (1993) Science 262:909.
  3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
  5. Chen, C. et al. (1994) J. Immunol. 152:4929.
  6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for B7-2/CD86 Antibody (AF-141-NA)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA Development, Flow Cytometry, IHC, IHC-P.


Filter By Application
ELISA Development
(2)
Flow Cytometry
(1)
IHC
(1)
IHC-P
(3)
All Applications
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Human
(6)
Mouse
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF-141-NA Applications Species
Popugailo, A;Rotfogel, Z;Levy, M;Turgeman, O;Hillman, D;Levy, R;Arad, G;Shpilka, T;Kaempfer, R; The homodimer interfaces of costimulatory receptors B7 and CD28 control their engagement and pro-inflammatory signaling Journal of biomedical science 2023-06-28 [PMID: 37381064] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Satoshi Horiba, Ryota Kami, Taiki Tsutsui, Junichi Hosoi IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human Skin JID Innovations 5/1/2022 [PMID: 35521044]
T. Zrzavy, R. Höftberger, T. Berger, H. Rauschka, O. Butovsky, H. Weiner et al. Pro‐inflammatory activation of microglia in the brain of patients with sepsis Neuropathology and Applied Neurobiology 4/1/2019 [PMID: 29804289]
Tobias Zrzavy, Joana Machado-Santos, Sheren Christine, Christoph Baumgartner, Howard L. Weiner, Oleg Butovsky et al. Dominant role of microglial and macrophage innate immune responses in human ischemic infarcts Brain Pathology 11/1/2018 [PMID: 29222823]
P Ai, Z Li, Y Jiang, C Song, L Zhang, H Hu, T Wang Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma Oncol Lett, 2017-06-22;14(2):2458-2462. 2017-06-22 [PMID: 28781683] (IHC-P, Human) IHC-P Human
T Zrzavy, S Hametner, I Wimmer, O Butovsky, HL Weiner, H Lassmann Loss of &#039;homeostatic&#039; microglia and patterns of their activation in active multiple sclerosis Brain, 2017-07-01;0(0):. 2017-07-01 [PMID: 28541408] (IHC-P, Human) IHC-P Human
Assunta Dal-Bianco, Günther Grabner, Claudia Kronnerwetter, Michael Weber, Romana Höftberger, Thomas Berger et al. Slow expansion of multiple sclerosis iron rim lesions: pathology and 7 T magnetic resonance imaging Acta Neuropathologica 1/1/2017 [PMID: 27796537]
Mansfield AS, Holtan SG, Grotz TE Regional immunity in melanoma: immunosuppressive changes precede nodal metastasis. Mod. Pathol., 2010-12-10;24(4):487-94. 2010-12-10 [PMID: 21151098] (IHC-P, Human) IHC-P Human
Schatton T, Schutte U, Frank NY, Zhan Q, Hoerning A, Robles SC, Zhou J, Hodi FS, Spagnoli GC, Murphy GF, Frank MH Modulation of T-cell activation by malignant melanoma initiating cells. Cancer Res., 2010-01-12;70(2):697-708. 2010-01-12 [PMID: 20068175] (IHC, Human) IHC Human
Sakthivel P, Shively V, Kakoulidou M The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm. J. Intern. Med., 2007-04-01;261(4):399-407. 2007-04-01 [PMID: 17391115] (ELISA Development, Human) ELISA Development Human
Show All 11 Publications.

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Secondary Antibodies

 

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Additional B7-2/CD86 Products

Blogs on B7-2/CD86.

Check out the latest blog posts on B7-2/CD86.
CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated ...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol CD86
Uniprot