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B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

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Western Blot: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis of CD80 in A) a partial recombinant protein and B) Daudi lysate using this antibody at 0.1 and 1 g/ml, respectively. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.
Western Blot: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis of CD80 in A) a partial recombinant protein and B) Daudi lysate using this antibody at 0.1 and 1 g/ml, respectively. Goat anti-mouse ...read more
Immunocytochemistry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Human thymus stained with CD80 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen. Note membrane and cytoplasmic ...read more
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis using the Alexa Fluor (R) 700 conjugate of NBP2-25255. Staining of CD80 in human PBMC (monocyte depleted) using anti-CD80 ...read more
Western Blot: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Analysis in human Raji lymphoma cells. Image from the standard format of this antibody.
Flow Cytometry: B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free [NBP2-80588] - Raji lymphoma cells. Image from verified customer review. Image from the standard format of this antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
62N3G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-25255AF350
Alexa Fluor 405
NBP2-25255AF405
Alexa Fluor 488
NBP2-25255AF488
Alexa Fluor 532
NBP2-25255AF532
Alexa Fluor 594
NBP2-25255AF594
Alexa Fluor 647
NBP2-25255AF647
Alexa Fluor 700
NBP2-25255AF700
Alexa Fluor 750
NBP2-25255AF750
Allophycocyanin
NBP2-25255APC
Biotin
NBP2-25255B
CoraFluor 1
NBP2-25255CL1
DyLight 350
NBP2-25255UV
DyLight 405
NBP2-25255V
DyLight 488
NBP2-25255G
DyLight 550
NBP2-25255R
DyLight 594
NBP2-25255DL594
DyLight 650
NBP2-25255C
DyLight 680
NBP2-25255FR
DyLight 755
NBP2-25255IR
FITC
NBP2-25255F
HRP
NBP2-25255H
Janelia Fluor 525
NBP2-25255JF525
Janelia Fluor 669
NBP2-25255JF669
PE
NBP2-25255PE
Unconjugated
NBP2-25255
mFluor Violet 450 SE
NBP2-25255MFV450
mFluor Violet 500 SE
NBP2-25255MFV500
mFluor Violet 610 SE
NBP2-25255MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-25255
BSA Free
NBP2-25255
BSA Free
NBP2-25255SS
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B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free Summary

Immunogen
Amino acids 102-223 of human CD80 were used as the immunogen for the antibody.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD80
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000. Use reported by customer review
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot reported by customer review
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27440778).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for B7-1/CD80 Antibody (62N3G8) - Azide and BSA Free

  • Activation B7-1 antigen
  • B7
  • B71
  • B7-1
  • BB1
  • CD28LG1
  • CD28LGB7-1 antigen)
  • CD80 antigen
  • CD80 molecule
  • CD80
  • costimulatory factor CD80
  • costimulatory molecule variant IgV-CD80
  • CTLA-4 counter-receptor B7.1
  • T-lymphocyte activation antigen CD80

Background

The CD80 antigen (B7-1, BB1) recognizes a 60-kD transmembrane glycoprotein that is a member of the immunoglobulin family. This molecule shares with CD86 the capability to be the ligand for two structurally similar molecules expressed on T lymphocytes, CD28 and CD152 (CTLA-4). CD80 antigen is expressed on in vitro activated B lymphocytes after 24h stimulation and at maximal level 48-72h post activation. It is not expressed on the majority of resting B cells from peripheral blood but identifies a subpopulation of B cells that has been previously activated. The antigen is also expressed by HTLV-1 transformed T cells activated monocytes, and constitutively on dendritic cells. CD80 binding provides costimulatory signals for T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD80: A co-stimulator of T cell activation
CD80 is a 60kD single chain type I transmembrane glycoprotein that is a member of the immunoglobulin family. CD80 is expressed on activated B- and T-lymphocytes, as well as a subpopulation of previously activated B-cells, but not on the majority of re...  Read full blog post.
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Bioinformatics

Gene Symbol CD80