ATPase Antibody [DyLight 650] Summary
Immunogen |
Produced in rabbits immunized with E. coli-derived Human ATPase fragment. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
DNAH8 |
Purity |
Antigen and protein A Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry
- Immunohistochemistry-Paraffin
|
Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. |
Buffer |
50mM Sodium Borate |
Preservative |
0.05% Sodium Azide |
Purity |
Antigen and protein A Affinity-purified |
Notes
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alternate Names for ATPase Antibody [DyLight 650]
Background
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for ATPase Antibody (NBP2-98188C) (0)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for ATPase Antibody (NBP2-98188C). (Showing 1 - 4 of 4 FAQ).
-
I have phosphate in my enzyme. What can I do?
- You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
-
I have 5% DMSO in my assay. Can I use PiColorLock Gold?
- Yes, the reagent is designed for drug screening work and other situations that require DMSO.
-
I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
- This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
-
I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
- Yes, only the phosphate will give a signal; pyrophosphate will not.
Secondary Antibodies
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Isotype Controls
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