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ATF4 Antibody (739441)

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Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATF4 Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB7218) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ATF4 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ATF4 Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB7218) followed by ...read more
ATF4 was detected in immersion fixed Jurkat human acute T-cell leukemia cell line using Mouse Anti-Human ATF4 Monoclonal Antibody (Catalog # MAB7218) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
739441
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7218-SP
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ATF4 Antibody (739441) Summary

Immunogen
E. coli-derived recombinant human ATF4
Met1-Pro351
Accession # P18848
Specificity
Detects human ATF4 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATF4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB7218 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ATF4 Antibody (739441)

  • activating transcription factor 4 (tax-responsive enhancer element B67)
  • Activating transcription factor 4
  • ATF4
  • cAMP-dependent transcription factor ATF-4
  • cAMP-responsive element-binding protein 2
  • CREB-2DNA-binding protein TAXREB67
  • cyclic AMP-dependent transcription factor ATF-4
  • Cyclic AMP-responsive element-binding protein 2
  • TAXREB67
  • TAXREB67CREB2cAMP response element-binding protein 2
  • Tax-responsive enhancer element-binding protein 67
  • TXREB

Background

ATF4 is an approximately 38 kDa member of the bzip family of transcription factors. It plays an important role autophagy, the unfolded protein response, and the response to oxygen and nutrient deprivation. These functions are regulated by the dimerization of ATF4 with the leucine zipper protein FIAT. ATF4 contains a basic DNA-binding domain (aa 280-300) and a leucine zipper domain (aa 306-334). Human ATF4 shares 87% aa sequence identity with mouse and rat ATF4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATF4 Antibody (MAB7218)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Bioinformatics

Gene Symbol ATF4
Uniprot