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ATF2 [p Thr71] Antibody (SR2159)

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Western Blot: ATF2 [p Thr 71] Antibody (SR2159) [NBP3-21538] - Western blot analysis using NBP3-21538 in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Anisomycin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
SR2159
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ATF2 [p Thr71] Antibody (SR2159) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human phospho-ATF2 [Thr71] (Uniprot #: P15336)
Modification
p Thr71
Specificity
Detects endogenous levels of phospho-ATF2 [Thr71]
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ATF2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:30
  • Western Blot 1:500-1:2000
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ATF2 [p Thr71] Antibody (SR2159)

  • activating transcription factor 2formerly
  • ATF2
  • cAMP responsive element binding protein 2
  • cAMP-responsive element-binding protein 2
  • CREB2
  • CREBP1
  • CRE-BP1
  • cyclic AMP-dependent transcription factor ATF-2
  • HB16
  • MGC111558
  • mXBP
  • TREB7

Background

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ATF2