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ATF2 [p Thr71] Antibody (4F2)

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Western Blot: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] - Positive Western Blot detected in 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or EGF). All lanes: ATF2 [p ...read more
Immunocytochemistry/ Immunofluorescence: ATF2 [p Thr71] Antibody (4F2) [NBP3-26431] - Staining of A549 cells(treated with 100mM EGF for 20min) with ATF2 [p Thr71] Antibody (4F2) at 1:63, counter-stained with DAPI. The ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4F2
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ATF2 [p Thr71] Antibody (4F2) Summary

Additional Information
Recombinant monoclonal antibody expressed in HEK293F cells
Immunogen
A synthesized peptide derived from Human ATF2 [p Thr71] [UniProt P15336]
Modification
p Thr71
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ATF2
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ATF2 [p Thr71] Antibody (4F2)

  • activating transcription factor 2formerly
  • ATF2
  • cAMP responsive element binding protein 2
  • cAMP-responsive element-binding protein 2
  • CREB2
  • CREBP1
  • CRE-BP1
  • cyclic AMP-dependent transcription factor ATF-2
  • HB16
  • MGC111558
  • mXBP
  • TREB7

Background

ATF-2 (activating transcription factor-2, CRE-BP1) is a transcription factor that binds the cAMP response element (CRE) and AP-1 response element and is abundantly expressed in the brain. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2 (2). SAPK and p38 MAPK can activate, in response to cellular stress, ATF-2 by phosphorylating this protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ATF2