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ARC/NOL3 Antibody [Unconjugated]

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Western blot shows lysates of rat and mouse skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat ARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human ARC Western Blot Standard (Catalog # WBC009) (1 ng) was included. A specific band for ARC was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western blot shows lysates of rat and mouse skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat ARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF636) followed by ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF636-SP
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ARC/NOL3 Antibody [Unconjugated] Summary

Immunogen
KLH-coupled human ARC synthetic peptide
CEPEAEAEPEPELE PEPDPEP
Specificity
Detects human, mouse, and rat ARC in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOL3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using AF636.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ARC/NOL3 Antibody [Unconjugated]

  • Apoptosis repressor with CARD
  • ARC
  • ARCFLJ35304
  • CARD2
  • Muscle-enriched cytoplasmic protein
  • MYC
  • MYP
  • NOL3
  • NOP30
  • NOPnucleolar protein 3
  • nucleolar protein 3 (apoptosis repressor with CARD domain)
  • Nucleolar protein of 30 kDa

Background

ARC [Apoptosis Repressor with CARD (Caspase-recruitment Domain)] is a 29 kDa cytosolic protein containing an N-terminal CARD joined to a C-terminal region rich in proline and glutamic acid. The expression of human ARC is primarily restricted to skeletal muscle and cardiac tissue. ARC inhibits apoptosis induced by Caspase-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARC/NOL3 Antibody (AF636)(1)


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Bioinformatics

Gene Symbol NOL3