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APPL Antibody (OTI4B11)

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Western Blot: APPL Antibody (OTI4B11) [NBP2-46536] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APPL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APPL1.
Western Blot: APPL Antibody (OTI4B11) [NBP2-46536] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APPL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Human lymphoma tissue using anti-APPL1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-APPL1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-APPL1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI4B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71782AF350
Alexa Fluor 405
NBP2-71782AF405
Alexa Fluor 488
NBP2-71782AF488
Alexa Fluor 532
NBP2-71782AF532
Alexa Fluor 594
NBP2-71782AF594
Alexa Fluor 647
NBP2-71782AF647
Alexa Fluor 700
NBP2-71782AF700
Alexa Fluor 750
NBP2-71782AF750
Allophycocyanin
NBP2-71782APC
Biotin
NBP2-71782B
CoraFluor 1
NBP2-71782CL1
DyLight 350
NBP2-71782UV
DyLight 405
NBP2-71782V
DyLight 488
NBP2-71782G
DyLight 550
NBP2-71782R
DyLight 594
NBP2-71782DL594
DyLight 650
NBP2-71782C
DyLight 680
NBP2-71782FR
DyLight 755
NBP2-71782IR
FITC
NBP2-71782F
HRP
NBP2-71782H
Janelia Fluor 525
NBP2-71782JF525
Janelia Fluor 549
NBP2-71782JF549
Janelia Fluor 646
NBP2-71782JF646
Janelia Fluor 669
NBP2-71782JF669
PE
NBP2-71782PE
PerCP
NBP2-71782PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71782
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APPL Antibody (OTI4B11) Summary

Immunogen
Full length human recombinant protein of human APPL1 (NP_036228) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
APPL1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
79.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for APPL Antibody (OTI4B11)

  • Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
  • adaptor protein containing pH domain, PTB domain and leucine zipper motif 1
  • adaptor protein, phosphotyrosine interaction, PH domain and leucine zippercontaining 1
  • AKT2 interactor
  • APPL
  • APPL1
  • APPLdip13-alpha
  • DCC-interacting protein 13-alpha
  • DIP 13 alpha
  • DIP13A
  • DIP13alpha
  • Dip13-alpha
  • KIAA1428
  • signaling adaptor protein DIP13alpha

Background

APPL is encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol APPL1