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Apolipoprotein E/ApoE Antibody (SC0536)

Images

 
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Analysis of paraffin-embedded human kidney tissue using anti-Apolipoprotein E antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Analysis of paraffin-embedded human liver tissue using anti-Apolipoprotein E antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Analysis of paraffin-embedded human tonsil tissue using anti-Apolipoprotein E antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Mouse brain tissue with Rabbit anti-Apolipoprotein E antibody at 1/1,000 dilution.The section was pre-treated using heat mediated ...read more
Immunohistochemistry-Paraffin: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - AD mouse brain tissue with Rabbit anti-Apolipoprotein E antibody at 1/1,000 dilution.The section was pre-treated using heat mediated ...read more
Western Blot: Apolipoprotein E/ApoE Antibody (SC0536) [NBP2-67565] - Analysis of Apolipoprotein E on human plasma tissue lysates with Rabbit anti-Apolipoprotein E antibody at 1/1,000 dilution.Lysates/proteins at 20 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
SC0536
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Apolipoprotein E/ApoE Antibody (SC0536) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within C-terminal human Apolipoprotein E/ApoE. (SwissProt: P02649 Human)
Localization
Secreted, extracellular space, extracellular matrix.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
APOE
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation Use at an assay dependent concentration
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Apolipoprotein E/ApoE Antibody (SC0536)

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Background

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol APOE